This site is fully related to medical laboratory information for public and health care professionals.
Introduction of Microbiology Reporting Methods Microbiology Reporting Methods are useful to provide similar report formats from different Micrbiology Laboratory platforms that should sound Micrbiologically clear and concise. It should also be compatible with International Guidelines of Clinical Microbiology Practice like Clinical & Laboratory Standards Institute …
Introduction of XLD Agar XLD agar is a selective, differential, and indicator medium for the isolation of enteric pathogens. XLD stands for xylose lysine deoxycholate. It also supports the growth of more fastidious enteric organisms. XLD agar was developed by Taylor. It was specially designed to allow the …
Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify S. aureus from clinical and non-clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective is due to very high salt (7.5%) compared with other media. Gram-Positive Staphylococcus: …
Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and Thiosulfate Citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar …
Introduction of Czapek Dox Agar Czapek medium also called Czapek’s agar (CZA) from the surname of inventor Czech botanist Friedrich Johann Franz Czapek (1902-1903). Later CZA was modified by another American inventor, chemist Arthur Wayland Dox (1910) and thus its name became’ Czapek-Dox medium’ and formula prepared …
Introduction of Dermatophyte Test Medium (DTM) Dermatophyte Test Medium (DTM) is a selective and differential medium recommended for the cultivation and isolation of pathogenic dermatophytic fungi. The Dermatophytes are a distinct group of fungi that infect the hair, skin, and nails in humans producing a variety …
Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA) is a fungal medium and its ingredients are clear from its name it contains potato ( vegetable), dextrose ( sugar), and agar ( solidifying agent). PDA is recommended for the isolation and enumeration of fungi …
AST Pattern of Bacteria AST Pattern of Bacteria covers common bacterial pathogens groups like Enterobacteriaceae, Staphylococcus, Enterococcus, Pseudomonas aeruginosa, Streptococcus pneumoniae, beta-haemolytic streptococci (Streptococcus pyogenes, Streptococcus agalactiae), Haemophilus species, Acinetobacter, Viridans group of streptococci, Shigella, and Neisseria species. Enterobacteriaceae Antimicrobial Agents- Zone Diameter (Intermediate) Extra …
Introduction of Microbiology Reporting Templates is collected to reduce the time and similarity for beneficiaries. This is a collection of templates regarding branches of microbiology like bacteriology, mycology, parasitology, virology, and immunology but most commonly bacteriology. List of Microbiology Reporting Templates Culture Negative Reports Positive …
Introduction of Microbial Diagnostic Disks Microbial Diagnostic Disks make it easy to presumptive identification of organisms. They are simple and easy to handle for the identification of bacteria in the Clinical Microbiology Laboratory. Some common examples of such disks are optochin, oxidase, and bacitracin. List …
Common Gram Negative Bacteria Biochemical Test Table Introduction of Gram-Negative Bacteria Biochemical Test ‘Common Gram Negative Bacteria Biochemical Test’ is a genuine puny collection of clinically relevant bacteria isolated in the clinical bacteriology laboratory by the creator and those etiological agents captured in his camera …
Introduction of Butyrate Disk Test Butyrate disk test is useful to differentiate Moraxella catarrhalis from Neisseria species like Neisseria gonorrhoeae, Neisseria lactamica. Principle of Butyrate Disk Test The butyrate disk test is a rapid test for the detection of the enzyme butyrate esterase. Bromochloro-indolyl butyrate (IB) is the substrate. Hydrolysis …
Introduction of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) test uses to identify the capability of organisms utilizing substrate, PYR by L-pyrroglutamyl amino-peptidase. Application of this test are as follows: Principle of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) acts as a substrate for the detection of the enzyme …
Introduction of API Test for Bacteria API test for bacteria as shown above picture is a 20-jumbo tests kit for a biochemical panel for the identification and differentiation of members of the family Enterobacteriaceae. API stands for Analytical Profile Index. It is an API 20E Test kit …
Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …
Introduction of Malonate Test Malonate test uses to check the ability of the organism to utilize malonate as the sole source of carbon for energy. To differentiate bacteria on the basis of a malonate utilization test. It is a colorful test to check the ability of …
Optochin Susceptibility Test Optochin Susceptibility Test uses for presumptive identification of Streptococcus pneumoniae from viridans streptococci. Principle of Optochin Susceptibility Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low concentration (5ug/ml). Optochin may also inhibit other viridians streptococci, but only …
Introduction of CAMP Test The CAMP test uses to identify preemptively Streptococcus agalactiae. It was first described in 1944 by Christie, Atkins, and Munch-Petersen, and the CAMP test is an acronym for their names. Principle of CAMP Test The beta lysin produced by Staphylococcus aureus is sphingomyelinase in nature, …
Introduction of Acetate Utilization Test Acetate Utilization Test ueses to determine the ability of an organism to use acetate as the sole source of carbon same as in the citrate utilization test for Enterobacteriaceae. It is the recommended assay for the differentiation of Shigella species from Escherichia coli. Composition of …
Introduction of Corn Meal Agar (CMA) Corn Meal Agar (CMA) is a well-established fungal medium that is a suitable substrate for chlamydospore production by Candida albicans and the maintenance of fungal stock cultures. This is a very simple formulation containing only cornmeal infusion and agar. The addition of glucose …
Oxidation-Fermentation (OF) Test Introduction Hugh and Leifson developed an Oxidation-Fermentation (OF) basal medium in 1953. It is a recommended medium for the detection of oxidation or fermentation of carbohydrates by bacteria. This basal medium is useful to aid in the identification of gram-negative bacteria on …
Introduction of Nitrate or Nitrite Reduction Test The nitrate or nitrite reduction test uses to determine the ability of an organism to reduce nitrate to nitrite. Anaerobic metabolism requires an electron acceptor other than atmospheric oxygen. Many gram-negative bacteria use nitrate as the final electron acceptor. The …
Introduction of DNase Test for Bacteria DNase tests positive and negative bacteria as shown below image. This test is useful for the presumptive identification of Staphylococcus aureus which produces the enzyme deoxyribonuclease (DNase) from other Staphylococci which do not produce DNase. This test is also positive in the following …
Introduction of ONPG Test ONPG test is useful in differentiating members of the family Enterobacteriaceae and genera Neisseria based on b-D-galactosidase activity. ONPG stands for o-Nitrophenyl-b-D-galactopyranoside. Here we discuss on ONPG Disk Test which is simple and easy to perform. Principle The ability of an organism to ferment lactose …
Introduction of MIU Test MIU test stands for motility indole urease test and it is used for three tests among them one for motility of bacteria, second for indole formation, and third for urea hydrolyzation test of bacteria. This assay is useful for the identification …
Introduction of Laboratory Instruments and Equipment Laboratory Instruments and Equipment make laboratory work easy and fast. Here we discuss the instruments and equipment of the Department of Pathology and Laboratory Medicine that includes Clinical Haematology, Clinical Biochemistry, Histopathology and Cytology, Clinical Microbiology and Immunology, Blood …
Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …
Introduction of Aeromonas hydrophila Aeromonas hydrophila is a gram-negative, facultative anaerobe, rod-shaped motile(due to single polar flagellum) bacterium without capsule and spore having a size from 0.3-1.0 µm wide by 1.0-3.5 µm long. It is generally found in the aquatic environment. Its presence can not …
Introduction of Enterobacter cloacae Enterobacter cloacae is a facultative anaerobic Gram-negative bacterium of size 0.3-0.6 x 0.8-2.0 μm. It is lacking capsule and spore and it is motile due to flagella which is a member of the Enterobacteriaceae family. E. cloacae is the normal gut …
Introduction of Shigella flexneri Shigella are Gram-negative bacilli measuring about 1-3 µm X 0.5 µm. They are non-motile, non-encapsulated, non-sporing, and non-acid-fast bacteria belonging to the family Enterobacteriaceae. They cause an infectious disease called Shigellosis. There are four species or serogroups based on biochemical and …
Introduction of Citrobacter koseri Citrobacter koseri is previously known as Citrobacter diversus. It is a facultative anaerobe of 1.0 × 2.0–6.0 μm in size, motile, non-capsulated, and gram-negative bacillus of the family Enterobacteriaceae. Citrobacter koseri acts as etiological agents of various pathological conditions like urinary tract infections (UTIs), …
Introduction Salmonella Paratyphi (serovar) belongs to Enterobacteriaceae and thus is a Gram-negative bacterium causing mild gastroenteritis to a life-threatening bloodstream infection. It causes paratyphoid fever and it is restricted to humans. Proper sanitation and hygiene may decrease its infection chance. Travelers must be cautious during …
The genus Proteus was discovered in 1885 by Hauser and it is also named after a Greek god. Proteus is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, motile, non-sporing rod-shaped bacterium without capsule and having a size of 0.4–0.8 μm in diameter and …
Introduction of Complete Thyroid Package Complete Thyroid Package is a group of clinical laboratory tests. It uses to say the effects of thyroid disorder (hypothyroidism or hyperthyroidism) on our body and their monitoring. Normal thyroid stimulating hormone (TSH) controls the basal metabolic rate of the …
Introduction of Mycology Mycology is the science that deals with the study of fungi whereas medical mycology is the science that deals with the study of fungi that causes disease is called medical mycology. General Features- Yeast Unicellular formSize-Width: 1-5 µmLength: 5-30 µmShape-Commonly oval shape …
Introduction of Aspergillus The genus Aspergillus has more than 180 species, among them, 38 are responsible to cause disease (able to grow at 37◦C. They are common in the environment. Aspergillus species are emerging pathogens which are ubiquitous molds that infect immunocompetent ( rarely) and immunocompromised …
Introduction of Microbiology and More Gallery Microbiology and More Gallery is a random collection hub of microbes and laboratory medicine-related footage. This is the genuine work of the author. Most of the pictures are commonly those encountered by the laboratory personnel during their working period. …
Introduction of SARS-CoV-2 SARS-CoV-2 is a positive-sense single-stranded RNA virus that causes coronavirus disease 2019 ( COVID -19). SARS-CoV-2 stands for severe acute respiratory syndrome coronavirus 2. On March 11, 2020, the World Health Organization (WHO) characterized COVID-19 as a pandemic. On March 11, 2020, …
Introduction of Obesity Package Obesity is the condition of being grossly fat or overweight. Whether people are suffering fromit or not is confirmed via body mass index (BMI). The common clinical laboratory tests that are beneficialto assay in this scenario are mentioned in the laboratory …
Introduction of Dengue Serotyping Real-Time PCR Test Dengue is a mosquito (Aedes aegypti)-borne viral disease. It is a major health threat in the tropical and sub-tropical territories of the world, particularly in urban and semi-urban areas. Approximately 50-100 million individuals have been infected with dengue …
Visiting Visa via Sponsorship Introduction If someone’s family member in Europe (particularly the Czech Republic), he/she wants to bring his family member through sponsorship various documents are mandatory. Some documents may vary according to the situation and relation. A visit visa is also called a …
Introduction Dengue is a mosquito (Aedes aegypti)-borne viral disease. It is a major health threat in the tropical and sub-tropical territories of the world, particularly in urban and semi-urban areas. Approximately 50-100 million individuals have been infected with this fever annually (WHO data) and more …
Introduction of Diabetes Package (Female) Diabetes Package (Female) is a combination of laboratory tests, radiological assays, and clinicians consultations regarding the worsening of health status in a female diabetic patient and this package will provide a clear concept for diabetic patients to control their diabetic …
Introduction of Hematoxylin and Eosin Stain Hematoxylin and eosin stain is abbreviated as H&E stain. It is one of the principal tissue stains used in histopathology to find out cancer from a suspected biopsy. The stain was first introduced by A. Wissowzky in 1876. The cell nuclei …
Introduction of Clinical Hematology Test Clinical Hematology is the branch of laboratory medicine pertaining to the study of blood, blood-forming organs, and blood diseases. Hematology tests include laboratory estimation of blood formation and blood disorders. e.g. Complete blood count (CBC)- A count of the total …
Introduction of Pap Test Pap test is also known as Pap smear and Pap stain. Pap stain was named after the three letters of the surname of developer George Papanicolaou in 1942 and was routinely used for cytological stain. It is the most common staining …
Introduction of Child Complete Health Checkup Package ‘Child Complete Health Checkup Package’ is a combination of both laboratories as well as radiological tests and clinicians (doctors’) consultations. This package will help to indicate the health status of a child. Related Assays of Child Complete Health …
Introduction of Exotoxins and Endotoxins Numerous bacteria produce toxins, enzymes, and pigments. Toxins and enzymes play significant roles in pathogenicity. Toxins are of two types- Differences Between Exotoxins and Endotoxins The differences between exotoxins and endotoxins are as follows- S. No Exotoxins Endotoxins 1. Exotoxins …
Introduction of Bacterial Endotoxin Detection by Chromogenic Method Bacterial Endotoxin Detection by Chromogenic Method uses for screening and diagnosis of Gram-negative bacterial infection with a low limit of detection is 1 pg/ml (0.005EU/ml). It also applies to the quantitative detection of endotoxin in pharmaceutical and …
Introduction of Fungus (1-3)-β-D-Glucan Test by Chromogenic Method Fungus (1-3)-β-D-Glucan Test by Chromogenic Method is used for screening and diagnosis of invasive fungal infection. It is a major cell wall component of most pathogenic fungi, used as a biomarker for the diagnosis of the invasive …
Introduction of Candida Mannan ELISA Detection Kit Candida Mannan ELISA Detection is used for the specific detection of Invasive Candidiasis and Candidemia. It works on the principle of competitive Enzyme-linked Immunosorbent Assay (ELISA). It is applied for the quantitative detection of Candida mannan antigen in …
Introduction of Aspergillus Galactomannan ELISA Detection Kit Galactomannan (GM) is a polysaccharide that is present in the cell wall component of Aspergillus species. It is released into the blood during fungal growth in the tissue that can be detected by Enzyme-Linked Immunosorbent Assay (ELISA). Principle …
Introduction of Immunochromatography Analyzer In Mycology Laboratory Immunochromatography Analyzer In Mycology Laboratory is an immunochromatography strip test system based on the photoelectric detection principle which should support the use of reagents based on the Gold Immunochromatography principle. With the suitable paper strip based on the …
Introduction of Automation System for Invasive Fungal Diagnostics/FACIS Full-Automatic Chemiluminescence Immunoassay System (FACIS) is an open system to detect the content of endotoxin, (1-3)-β-D-glucan and galactomannan in bronchoalveolar lavage (BAL) fluid or serum clinical samples. With both a photometry system and chemiluminescence system built in, …
Introduction of Nosocomial Infections Nosocomial infections are also called hospital-acquired infections (HAI) that occur during hospital stay and microbes are the causative agent of such infections. Common Nosocomial Infections The most commonly occurring nosocomial infections are as follows- Microbial Etiological Agents of Common Nosocomial Infections …
Introduction of SARS‑CoV‑2 RNA Extraction using Biocomma Kit Biocomma nucleic acid (DNA/RNA) extraction kit is a Chinese product that is useful for RNA extraction systems for COVID-19 detection. It is applied for manual extraction and it is completed in four steps- A kit (for 50 …
Introduction of Dengue IgG and IgM Rapid Test Dengue rapid test (RDT) uses for the detection of immunoglobulin G(IgG) and immunoglobulin M (IgM) where IgM is responsible for the past while IgG is for present infection as shown above picture. Dengue is a mosquito-borne viral …
Indole Production test This indole test is useful to identify the bacteria on the basis of indole production. The list of indole-positive organisms is- Principle of Indole test The ability of an organism to split Indole from the amino acid tryptophan is due to the presence of …
Introduction of Whole Body Checkup The ‘Whole Body Checkup’ is beneficial from the following points of view- Related Assays Package Contents- A whole Body Checkup includes the following tests:- Keynotes on Whole Body Checkup
Introduction Alkaptonuria is an enzyme deficiency, homogentisic dioxygenase (HGD) rare inherited disorder. It is used to break down a toxic substance called homogentisic acid. Excess of homogentisic acid causes bones and cartilage to become discolored and brittle which causes later osteoarthritis, especially in the spine …
Introduction of Robertson’s Cooked Meat (RCM) Medium Robertson’s Cooked Meat (RCM) Medium is the recommended medium for the cultivation of anaerobic as well as aerobic bacteria. It is especially for pathogenic Clostridium species from clinical, food, and water samples. It can also be used as a maintenance medium …
Introductions of Common Urinary Casts Urine casts in urine routine examinations are completed in the following steps- First request the patient to bring a urine specimen. After the arrival of the specimen in the clinical laboratory, take nearly 5 ml of urine and centrifuge it. …
Introductions of Common Urinary Crystals Urinary crystals in urine during the routine examination can appear in the following steps- First request the patient to bring a urine sample. After the arrival of the sample/specimen in the clinical laboratory, take nearly 5 ml of urine. Centrifuge it …
Introduction of Hot air oven A hot air oven is the most common method of sterilization in the laboratory working on dry heat. Sterilization is the process of removing or destroying all microorganisms including viruses, bacteria, and their spores from the article or surface without …
Introduction of Autoclave An autoclave is the most common method of sterilization in the laboratory working on moist heat. Sterilization is the process of removing or destroying all microorganisms including viruses, bacteria, and their spores from the article or surface without destroying its quality and …
Introduction of Incubator The incubator is an electrical device in which the incubation process is performed which conditions an enclosure to a predetermined temperature since it provides and maintains all artificial optimal conditions for the growth of microbial culture as well as cell and tissue …
Introduction of BHI Broth Brain Heart Infusion (BHI) broth is a highly nutritive medium and is thus useful for cultivating a wide variety of microorganisms like aerobic and anaerobic bacteria, yeasts, and molds. BHI broth is commonly used in blood culture work and allied pathological …
Introduction of RF Test RF test uses to detect autoantibody of rheumatoid factor which stands for rheumatoid factor. Rheumatoid arthritis ( RA) is a chronic systemic inflammatory disorder that may affect many tissues and organs like skin, blood vessels, heart, lungs, and muscles but particularly …
Introduction of ASO Test ASO stands for Antistreptococcal O and it is an antibody targeted against streptolysin O, a toxic enzyme produced by group A Streptococcus bacterium. Antistreptococcal O and anti -DNase are the most common of several antibodies that are produced by the body’s immune system in response to a strep infection …
Introduction of ELISA ELISA stands for Enzyme-Linked Immunosorbent Assay. It comes under antigen and antibody reaction tests and is useful for the identification of antigens or antibodies of the following specimens serum, urine, CSF, sputum, semen, supernatant of culture, stool, etc. It is also applicable …
Introduction of C-Reactive Protein (CRP) Test C-Reactive Protein (CRP) is a carbohydrate-reactive protein. The C-Reactive Protein (CRP) Test was so named because it was first identified as a substance in the serum of patients with acute inflammation that reacted with the somatic ‘C’ carbohydrate antigen …
Introduction of Food Intolerance Package The food Intolerance package includes a panel of tests to know if patients have sensitivity to a specific food. A food allergy test is also a part of the Food Intolerance package to know the reason for intolerance in which …
Introduction of Rapid Plasma Reagin (RPR) Test RPR is a screening non-specific test for syphilis caused by a bacterium, Treponema pallidum. It has replaced the VDRL test due to having properties like no need of preparing fresh reagents daily, no need for a microscope, and …
Introduction of TPHA Test TPHA test stands for Treponema pallidum hemagglutination. This is a confirmatory test for syphilis that is caused by a bacterium, T. pallidum. TPHA test is a sensitive indirect hemagglutination test, that detects specific Treponema pallidum antibodies in serum within one hour. It uses …
Introduction of Master List of Records in Clinical Laboratory ‘Master List of Records in Clinical Laboratory’ is an important document to reflect the quality of laboratory that is required during ISO accreditation as well as national accreditation. It is also required during laboratory renewable. It …
Introduction of VDRL Test VDRL stands for Veneral disease research laboratory. The laboratory was renamed the Treponemal Pathogenesis and Immunology Branch of the United States Public Health Service. The Venereal Disease Research Laboratory (VDRL) test is one of two variations of micro flocculation procedures used for serological testing …
Introduction of Troponin Test The high-sensitivity cardiac troponin test (hs-cTnT) is the new generation of heart/cardiac enzyme assaying that allows for the detection of very low levels of troponin T, assisting to diagnose heart attacks more quickly. It works on the principle of immunochromatography. It …
Introduction of Category A and B laboratory List in Nepal There are two types of medical/clinical laboratories in Nepal. One is a government laboratory while another is a private laboratory. The private laboratory can not proceed with the tests without the accreditation of government bodies …
Introduction of Staphylococcus aureus Scientific classification of Staphylococcus aureus is given as: Domain Bacteria Phylum Firmicutes Class Bacilli Order Bacillales Family Staphylococceae Genus Staphylococcus Species aureus Table: Biological Classification of Staphylococcus aureus Staphyle means bunch and Kokko mean berry. Staphylococcus aureus was isolated by Pasteur (1880) from a pus sample. …
Introduction of CLED Agar CLED stands for cysteine, lactose, and electrolyte-deficient. CLED agar or medium is a differential culture medium used for the isolation and enumeration of bacteria in urine specimens from suspected cases of urinary tract infection (UTI). It favors the growth of all …
Introduction of Preventive Health Packages ‘Preventive Health Packages’ are group tests and a combination of doctor check-ups for people. To know the health status and to save their lives and follow the quotation, ‘prevention is better than cure, these packages are really beneficial. A family …
Introduction Radiology Department or section is an integral part of any hospital for disease diagnosis or other health problems diagnosis. The most common is while X-ray advanced tool, Magnetic resonance imaging (MRI) helps clinicians to treat patients in various ways. Computerized tomography (CT)-scan is also …
Introduction of Well Women Check-up Package ‘Well Women Check-up Package‘ is commonly advised the women who are aged 30 plus. This package is a combination of clinician/doctor and diagnostic tests to find out the health status of an individual. A family physician consultation will help …
Introduction ‘Advanced Senior Health Package’ is a group test for senior citizens. This group of people is more prone to diseases/infections. To save their lives, do not ignore follow the quotation, ‘prevention is better than cure. This package is really beneficial. Advanced Senior Health Package …
Introduction of USG USG stands for ultrasonography. It is also called an ultrasound scan and it is a common clinical test thatuses high-frequency sound waves to take live images from the inside of the body. It is also safe fromother imaging techniques due to lacking …
Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …
Phenylalanine Deaminase (PDA) Test PDA stands for phenylalanine deaminase and PDA tests positive and negative are as shown below footage. It is used in the differentiation of gram-negative enteric bacilli based on the ability of the microorganisms to produce phenyl pyruvic acid by oxidative deamination. The deamination of …
Introduction Double A Cafe (DAC) is situated in the heart of Kathmandu surrounded by Labim mall (Pulchowk), Krishna Mandir/Patan Durbar Square, Shri Bangalamukhi Temple, and Patan Doka touching Pimpahal Pokhari from the south and west directions. It is one of the perfect cafes for those …
Introduction of Hospitals in Kathmandu Kathmandu is made up of three districts and they are Kathmandu, Lalitpur, and Bhaktapur, together with expanding an area of 899 square kilometers. It is the largest and busiest city in Nepal and thus has numerous reputed health care and …
Introduction of CT KUB A CT KUB stands for computerized tomography of the kidneys, ureters, and bladder. The use of the CT KUB scan is to obtain images from various angles of the urinary system and its surrounding structures, that enclose the area from the …
Introduction of CT IVU The full form of CT IVU is computed tomography-intravenous urography and it is also called CT urography. It is a technique that uses CT Scanners to take images of the genitourinary tract (organs like kidneys, ureters, and bladder). This examination or …
Introduction of Vitamin-D Vitamin D is not a vitamin but a hormone or prohormone that is essential for maintaining healthy bones and teeth. It also helps in regulating inflammation, immune function, intestinal calcium absorption, and maintaining adequate blood levels of calcium and phosphorus, which are …
Introduction Cholera is an infectious disease that is caused by the Gram-negative bacterium, Vibrio cholerae. It is responsible for severe watery diarrhea, which can lead to dehydration and even death if untreated. Cholera occurs due to eating food or drinking water contaminated V. cholerae. 13 …
Introduction of Health Benefits of Eating Fish Fish is a part of a healthy diet since it has a low-fat high-quality protein with omega-3 fatty acids and vitamins (like vitamin D, and riboflavin i.e. B2). It is also rich in calcium and phosphorus and has …
Introduction of Thyroid Scan A thyroid scan or thyroid scintigraphy is a diagnostic nuclear medicine assay that provides information regarding the structure and function of the thyroid gland and this technique makes it able to see the size, shape, function, and position of the gland. …
Introduction of HLA-B27 Test HLA-B27 stands for Human leukocyte antigen B27. It is a protein found on the surface of our white blood cells (WBCs) and it is a blood test that identifies HLA-B27 proteins. Although most human leukocyte antigens (HLAs) protect your body from …
Introduction of Glucose Tolerance Test The glucose tolerance test (GTT), also known as the oral glucose tolerance test (OGTT) is useful to measure the body’s response to sugar (glucose)and it can be used to screen for type 2 diabetes. More commonly, a modified version of …
Introduction of Glucose Challenge Test The glucose challenge test (GCT) measures the body’s response to glucose (sugar) and it is tested during pregnancy to screen for gestational diabetes (diabetes that develops during pregnancy). The GCT is done in two steps. Initially drink a glass of …
Introduction of PSA Test PSA stands for prostate-specific antigen and it is a proteinous substance produced by prostate cells.It is a marker of prostate cancer. Its normal range in our blood is less than 4 nanograms. per milliliter. Range of PSA Test Normal Range: 4.0 …
Thyroid Scan Report Introduction Thyroid CT Scan/Thyroid Computed Tomography Imaging is a technique used to scan the thyroid gland that controls metabolism. It is a small, butterfly-shaped gland located in the middle of the lower neck having normal size of about 2 cm thick, 3 …
Introduction of Health Laboratory Establishment and Operation As we know, ‘Laboratory Diagnosis’ is the third eye of doctors/clinicians that confirms the cause of pathology/ disease. It highlights the importance of Health Laboratory Establishment and Operation for better service. Here, we discuss in brief the national …
Introduction HSG stands for hysterosalpingography and it is an X-ray test to lay out the internal shape of the uterus and show whether the fallopian tubes are blocked. Uses of HSG Test Procedure of HSG A tiny tube is threaded through the vaginal and cervical …
Introduction of MRI Scan MRI stands for magnetic resonance imaging and it uses magnetism, radio waves, and a computer to produce images of body structures. It provides essential information on various internal organs inside the body. It can be applied as a diagnostic tool to …
Introduction DTPA stands for diethylenetriaminepentaacetic acid and it is an amino polycarboxylic acid consisting of a diethylenetriamine backbone with five carboxymethyl groups. DTPA Renal Scan is used to know how the left and right kidneys work comparatively. This test also recognizes any sites of blockages …
Introduction of CT -Scan CT stands for computed tomography and it is an imaging technique that combines a series of X-ray images taken at different angles and uses a computer to create images from these x-rays. It is widely available, and fast, and provides a …
Introduction of Gene Sequencing Deoxyribonucleic acid (DNA) sequencing is the process of finding out the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology (Sanger/Next-generation sequencing) that is used to find out the order of the four bases …
Introduction of Monkeypox Virus Monkeypox is a rare disease and its causative agent is the monkeypox virus (MPXV). This virus is a double-stranded DNA and it belongs to the Orthopoxvirus genus which includes the variola (smallpox) virus as well as the vaccinia virus, which is …
Introduction of Microbiology and Serology Laboratory Here we are discussing not general microbiology but medical/clinical microbiology it is a discipline that encloses a wide range of testing methodologies, and it is complex in terms of organisms (bacteria, viruses, fungi, parasites) and methods used to isolate …
Introduction of Immunology Laboratory The Immunology Laboratory is useful for laboratory testing and clinical consultation in several broad domains including the evaluation of autoimmune disease, immunodeficiencies, immunoproliferative disorders, and allergies, as well as having responsibility for some senses of infectious disease serology. To provide absolute …
Introduction of Histopathology and Cytopathology Laboratory Histopathology/histology is the branch of medical science that involves the examination of sampled whole tissues under a microscope. The size of the histopathological specimens is of three types, small, medium, and large. An autopsy is also called a post-mortem …
Introduction of MacConkey Agar MacConkey agar (MAC) uses for the culture of gram-negative bacteria and therefore Enterobacteriaceae belonging bacteria grow well on this medium and coliforms also enjoy this medium. MAC is a modification of Neutral Red Bile Salt Agar developed by MacConkey. It was one of the …
Introduction of Medical Laboratories in Nepal There are two types of medical/clinical laboratories in Nepal. One is a private laboratory while another is a government laboratory but both are monitored by National Public Health Laboratory (NPHL). NPHL is the government national reference laboratory under the …
Introduction of Molecular Diagnostics Laboratory As we know, simple diseases are due to either metabolic disorders or infectious agents (bacteria, viruses, fungi, parasites, prions). Molecular Diagnostics is a powerful tool for the identification of both conditions at genetic levels (e.g. metabolic disorders like cancer, and …
Introduction of Cytogenetic Test The cytogenetic assay is useful for chromosome analysis, syndromes like Williams, Prader-Willi, Angelman, Miller-Dieke, Smith-Magenis, and DiGeorge, and hematological malignancies detection. List of Common Tests Test Method Application Karyotyping chromosome analysis e.g. neonatal Karyotyping, Product of Conception (POC) karyotyping Fluorescent InSitu …
Importance of Clinical Lab Tests As we know, lab diagnosis is the third eye of clinicians/doctors. Medical/ clinical laboratory tests are important for us as a part of a routine checkup to look for changes in our health. These assays also assist to diagnose medical …
Introduction of Glucose Challenge Test(GCT) The glucose challenge test (GCT) determines our body’s response to glucose (sugar). The GCT is done during pregnancy to screen for gestational diabetes that develops during pregnancy. GCT Testing Time It is generally tested between 24 and 28 weeks of …
Introduction of Serological Tests Serological tests also known as serology tests or antigen or antibody tests are any of several laboratory procedures carried out on blood specimens (the serum-an amber-colored, protein-rich fluid that separates out when blood coagulates) for the purpose of detecting antibodies or …