Penicillium cheresanum: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Penicillium cheresanum: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Penicillium cheresanum is a species of mold belonging to the genus Penicillium, commonly found in soil and decaying organic matter. Moreover, it thrives in a variety of environments, demonstrating its adaptability to different ecological conditions. In addition, this mold is known for producing secondary …

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Creatinine Test- Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Creatinine Test- Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Creatinine Test measures the levels of creatinine in blood or urine, reflecting kidney function and overall health. Moreover, creatinine is a waste product formed from muscle metabolism and excreted by the kidneys. In addition, this test helps evaluate kidney efficiency, determining how well …

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Cholesterol Test- Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Cholesterol is a waxy substance found in your blood, playing a vital role in building cells. Moreover, it helps produce hormones, vitamin D, and digestive substances like bile acids. In addition, your liver produces cholesterol, but it also comes from dietary sources like meat …

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D-Zone Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

D-Zone Test Positive strain of Staphylococcus aureus

Introduction The D-Zone test identifies inducible clindamycin resistance in bacteria. Typically, it involves using erythromycin and clindamycin discs on an agar plate. When placed near each other, the interaction is observed. If a D-shaped zone appears around clindamycin, resistance is confirmed. Consequently, this test helps …

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Rifampicin Resistant Indeterminate- Introduction, Result, Cause, Implication, and Summary

Rifampicin Resistant Indeterminate result of Mycobacterium tuberculosis

Introduction “Rifampicin resistant indeterminate” refers to an unclear or inconclusive result in testing for resistance to the antibiotic rifampicin, often used in the treatment of tuberculosis (TB). Here’s a breakdown of the term: Rifampicin Resistant Result and Cause Indeterminate Result: This means the test did …

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D-Dimer Test-Introduction- Test Result, Unit, Reference Range, Method, and Keynotes

D-Dimer Test-Result, Unit, Reference Range, and Method

Introduction The D-Dimer test measures a substance released when a blood clot breaks up. It helps diagnose clotting disorders. Doctors often order this test to rule out conditions like deep vein thrombosis. It is also useful for diagnosing pulmonary embolism. A high D-Dimer level indicates …

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Extra-Pulmonary Tuberculosis-Introduction, Types, and keynotes

Acid fast bacilli of Mycobacterium tuberculosis in urine sediment

Introduction Extra-pulmonary tuberculosis (EPTB) affects areas outside the lungs. It impacts the lymph nodes, pleura, and bones. Patients often experience varied symptoms, depending on the affected site. Lymph node TB causes swelling, while bone TB results in pain. Pleural TB leads to chest pain and …

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Comparative Analysis of Capsule Expression in Cryptococcus neoformans: India Ink Visualization from Clinical CSF Samples versus SDA Agar Cultures

Cryptococcus neoformans capsules in India Ink Preparation of Culture of organisms

Introduction of India Ink preparation Cryptococcus neoformans is an encapsulated yeast that causes cryptococcal meningitis, particularly in immunocompromised individuals. The capsule is a critical virulence factor, and its visualization is essential for diagnosis. This preparation is a common method used to visualize the capsule. Here, …

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Presence of Numerous Pus Cells Without Detectable Microbes in Gram-Stained Sputum: Introduction, Diagnostic Implications, and Clinical Considerations

Presence of Numerous Pus Cells Without Detectable Microbes in Gram-Stained Sputum: Introduction, Diagnostic Implications, and Clinical Considerations

Introduction Gram staining is a routine diagnostic tool for respiratory infections. It reveals bacteria in sputum samples. Occasionally, Gram staining shows numerous pus cells but no microbes. This finding raises several clinical questions and concerns. Pus cells indicate an inflammatory response. They suggest a possible …

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Enterococcus gallinarum – Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

E. gallunarum in Gram stain of culture showing Gram positive cocci in pairs

Introduction Enterococcus gallinarum is a Gram-positive, facultatively anaerobic bacterium. It naturally inhabits the intestines of humans and animals. This bacterium exhibits intrinsic resistance to vancomycin. It can cause infections in immunocompromised patients. It is associated with urinary tract infections and bacteremia. Proper laboratory techniques are …

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Cedecea davisae- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Cedecea in wet mount of culture microscopy

Introduction Cedecea davisae is a rare, opportunistic pathogen in the Enterobacteriaceae family. It was first isolated from a human wound. This bacterium can cause infections in immunocompromised individuals. It exhibits resistance to several antibiotics. It is often misidentified due to its rarity. However, proper laboratory …

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Staphylococcus epidermidis- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Staphylococcus epidermis growth on blood agar

Introduction Staphylococcus epidermidis is a common, coagulase-negative bacterium found on human skin and mucous membranes. It is part of the normal human flora. However, it can cause infections, particularly in immunocompromised individuals. Notably, it is a leading cause of hospital-acquired infections. These infections often occur …

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Gemella morbillorum-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Gemella morbillorum in Gram staining of culture microscopy

Introduction Gemella morbillorum is a gram-positive bacterium, typically found in the human mouth, throat, and respiratory tract. It occasionally causes infections, particularly in immunocompromised individuals or those with underlying health conditions. Common infections include endocarditis, bacteremia, and abscesses. Notably, it can lead to serious complications …

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Fungal Typing, Sequencing, and AFLP in Clinical Mycology Lab-Introduction, Purpose, Method, Application, and Conclusion

Various Candida species growth on Chromagar-Candida albicans, Candida krusie, Candida tropicalis, and Candida glabrata

Introduction to Advanced Molecular Techniques in Clinical Mycology Clinical mycology laboratories play a crucial role in diagnosing and managing fungal infections. Traditional methods like culture and microscopy are often complemented by advanced molecular techniques to improve the accuracy and speed of fungal identification. Among these …

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Visa Process Documents Required to Attend a Conference in the USA from Nepal- Introduction, Visa Application Process, and Conclusion

Introduction If you are a citizen of Nepal planning to attend a conference in the USA, you will typically apply for a B-1 Business Visitor visa. Visa Application Process Here is a detailed list of the documents you will need for the visa application process: …

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Kaplan-Meier Survival Curves- Introduction, Applications, and Keynotes

Kaplan-Meier Survival Curves- Introduction, Applications, and Keynotes

Introduction Kaplan-Meier survival curves estimate survival over time from lifetime data. They plot survival probabilities against time. Each event in a study recalculates these probabilities. The curves step down with each event, staying flat during event-free times. Vertical marks on the curve indicate censored data, …

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Skill for Searching Proper Journal Article: Introduction, Method, and Keynotes

Skill for Searching Proper Journal Article: Introduction, Method, and Keynotes

Introduction of Skill for Searching Proper Journal Article When looking for the right journal article, start by defining your research question clearly. Use academic databases like PubMed or JSTOR to find relevant journals. Apply specific keywords related to your topic to narrow down the search …

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Journal Search Engine: Introduction, Common List, and Keynotes

Journal Search Engine: Introduction, Common List, and Keynotes

Introduction A journal search engine allows users to find academic articles and scholarly literature. It typically accesses multiple databases. Researchers use these platforms to locate relevant studies, papers, or data quickly. Most engines offer advanced search options to refine results by date, subject, or type. …

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PhD Semester Progress Report- Introduction, Contents, and Keynotes

PhD Semester Progress Report- Introduction, Contents, and Keynotes

Introduction Writing a PhD semester progress report involves summarizing your academic and research activities over the semester. This report is important for tracking your progress, identifying challenges, and planning future work. Contents for PhD Semester Progress Report Here’s a guide on how to structure and …

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Candidiasis-Introduction, Types, Signs and Symptoms, Causative Agent, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Candida albicans growth on SDA after 2 days of incubation

Introduction Candidiasis is an infection caused by Candida fungi, commonly Candida albicans. These fungi naturally live on the skin and inside the body, like in the mouth, throat, gut, and vagina, without causing problems. However, if the environment inside the body changes or the immune …

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Iron Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction An iron blood test measures how much iron is in your blood. It helps diagnose conditions like anemia or iron overload. This test can show if you’re absorbing iron properly. Doctors often order it when symptoms like fatigue or weakness appear. The test involves …

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Staphylococcus hominis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Staphylococcus hominis colony morphology on blood agar

Introduction Staphylococcus hominis is a type of bacteria commonly found on human skin. It is part of the natural skin flora, living harmlessly in most cases. However, it can cause infections, especially in people with weakened immune systems or with implanted medical devices. These infections …

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Aeromonas salmonicida- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Aeromonas salmocida growth on MacConkey agar of sputum culture

Introduction Aeromonas salmonicida is a notorious bacterium primarily targeting fish, causing the deadly disease known as furunculosis. Typically found in freshwater and marine environments, it poses a significant threat to aquaculture industries worldwide. This bacterium spreads quickly, particularly in crowded conditions like those found in …

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Cancer and Oncology Patient: Introduction, Differences, and Summary

Introduction The terms “oncology patient” and “cancer patient” are closely related but have slightly different implications based on context and usage. Differences Cancer Patient: Oncology Patient: Oncology is the branch of medicine that deals with the prevention, diagnosis, and treatment of cancer.Thus, an “oncology patient” …

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Raoultella ornithinolytica: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Routella ornithinolytica lactose fermenting colony on MacConkey agar

Introduction Raoultella ornithinolytica is a Gram-negative, encapsulated, non-motile, rod-shaped bacterium belonging to the family Enterobacteriaceae. Originally classified under the genus Klebsiella, it has been reclassified due to its distinct biochemical properties, including the ability to metabolize ornithine. This bacterium is commonly found in water, soil, …

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Leuconostoc mesenteroides-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Leuconostoc mesenteroides in wet mount of culture microscopy at a magnification of 1600X

Introduction Leuconostoc mesenteroides is a species of lactic acid bacteria that plays a significant role in food fermentation and preservation. It is characterized by its ability to convert sugars into lactic acid, a process that enhances the shelf life and safety of many foods. This …

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Journal Articles Summarizing Tips-Introduction, Major Steps Used, and Keynotes

Journal Articles summarizing Tips-Introduction, Major Steps Used, and Keynotes

Introduction of Journal Articles Summarizing Tips Summarizing long journal articles efficiently requires a strategic approach to reading and understanding the material. It will minimizes the time and provides great output. Major Steps Used for Journal Articles Summarizing Tips Here’s a comprehensive guide to help you …

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Streptococcus sanguinis-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Streptococcus sanguinis colony morphology on blood agar of urine culture

Introduction Streptococcus sanguinis is a Gram-positive bacterium belonging to the Streptococcaceae family. It is a commensal bacterium commonly found in the human oral cavity and is part of the normal oral microbiota. While it generally coexists peacefully with its host, S. sanguinis can also play …

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Declaration of Helsinki- Introduction, Purpose, Key Points, and Summary

Declaration of Helsinki- Introduction, Purpose, Key Points, and Summary

Introduction of Declaration of Helsinki The Declaration of Helsinki is a set of ethical guidelines and principles for conducting human clinical trials and biomedical research involving human subjects. It was first adopted in 1964 by the World Medical Association (WMA) and has undergone several revisions …

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Quardruple Test: Introduction, Normal range, Clinical Significance, and Keynotes

Quardruple Test: Introduction, Normal range, Clinical Significance, and Keynotes

Introduction The Quadruple Test, also known as the Quad Test or Quadruple Screen, is a prenatal screening test conducted during the second trimester of pregnancy to assess the risk of certain chromosomal abnormalities and neural tube defects in the developing fetus. The test typically involves …

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Open System versus Closed System Software-Introduction, Applications, and Keynotes

Open System versus Closed System Software-Introduction, Applications, and Keynotes

Introduction Open systems and closed systems refer to different approaches in the development and distribution of software. These terms are often used in the context of operating systems, but they can also apply to software applications and other computing environments. Here’s a brief overview of …

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Operating Systems-Introduction, Types, Uses, and Keynotes

Operating Systems-Introduction, Types, Uses, and Keynotes

Introduction An operating system (OS) is software that acts as an intermediary between computer hardware and the user. It provides a platform for running applications and managing computer resources. The primary functions of an operating system include: Common examples of operating systems include Microsoft Windows, …

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R (programming language)-Introduction, Application, and Keynotes

R (programming language)-Introduction, Application, and Keynotes

Introduction of R (programming language) R is a programming language and environment specifically designed for statistical computing and data analysis. It was created by Ross Ihaka and Robert Gentleman at the University of Auckland, New Zealand, and is currently maintained by the R Development Core …

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Microsoft Excel-Introduction, Uses, and Keynotes

Microsoft Excel-Introduction, Uses, and Keynotes

Introduction Microsoft Excel is a powerful spreadsheet software developed by Microsoft that allows users to create, organize, analyze, and visualize data in tabular form. It is widely used in various industries, businesses, and educational institutions for tasks ranging from simple data entry to complex financial …

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Manual RBCs count using Neubauer Chamber

Manual RBCs count using Neubauer Chamber

Introduction Counting red blood cells (RBCs) using a Neubauer chamber, also known as a hemocytometer, is a manual technique commonly used in hematology. Here’s a step-by-step guide on how to perform a manual RBC count using a Neubauer chamber: Equipment and Materials Procedure Keynotes on …

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Reference Manager Software: Introduction, Types, Handling Procedure, Keynotes

Introduction Reference manager software, also known as bibliographic software or citation management software, is a type of tool used for managing and organizing references, citations, and bibliographies in academic writing and research. The primary purpose of these software tools is to simplify the process of …

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Copper Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The blood Copper Test is a clinical diagnostic procedure used to measure the concentration of copper in the blood. This test is important in the assessment of various health conditions related to copper metabolism. Test Result, Unit, Reference Range, and Test Methods The blood …

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Acid Phosphatase Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Acid Phosphatase Test is a blood test primarily used to measure the level of acid phosphatase enzyme in the blood. Acid phosphatase is an enzyme found in several tissues, including the prostate gland, liver, spleen, blood cells, and bone marrow. Test Result, Unit, …

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Gemella-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Gemella morbillorum colony morphology on blood agar of blood culture

Introduction Gemella is a genus of bacteria that is part of the normal flora of the human body, particularly found in the oral cavity, gastrointestinal tract, and upper respiratory tract. These bacteria are typically harmless but can be opportunistic pathogens, especially in individuals with compromised …

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Serum Osmolality/Osmolarity-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Serum osmolality, often referred to as osmolarity in clinical practice, is a measure of the solute concentration in the blood. It is an important laboratory test that reflects the body’s hydration status and the balance of solutes and water. Here’s a brief introduction: Definition: …

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JCR Impact Factor-Introduction, Keynotes, and Applications

Introduction The Journal Citation Reports (JCR) Impact Factor is a widely recognized metric used to measure the influence of academic journals. Keynotes Here are key points about the JCR Impact Factor: Applications The Journal Citation Reports (JCR) Impact Factor is a prominent metric in the …

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SCImago Journal Rank-Introduction, Keynotes, and Applications

Introduction The SCImago Journal Rank (SJR) is a measure reflecting the scientific influence of scholarly journals. It accounts for both the number of citations received by a journal and the importance or prestige of the journals where such citations come from. Keynotes Here are the …

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Medical Fungi Name Changes-Introduction, and Name Lists

Medical Fungi Name Changes List-1

Introduction The dynamic field of fungal phylogenetics is continually evolving, leading to numerous revisions in the classification and nomenclature of fungi. The list provided here captures several of the latest name changes among fungi of medical significance. However, it is important to note that this …

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Turbidimetry-Introduction, Principle, Test Requirements, Result-Interpretation, Application, and Keynotes

Introduction Turbidimetry is an analytical technique used to measure the turbidity (cloudiness or haziness) of a fluid due to the presence of suspended particles. The basic principle of turbidimetry is to measure the loss of intensity of light due to the scattering effect of the …

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Immunofixation electrophoresis (IFE) Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Immunofixation electrophoresis (IFE) is a laboratory technique used primarily in the diagnosis and monitoring of certain blood disorders, particularly those involving abnormal or excessive production of proteins by the immune system. Here’s a brief introduction: This test is a sophisticated tool in the field …

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Anti-alpha-1 Antitrypsin Antibodies-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Alpha-1 antitrypsin (AAT) is a blood protein that functions as a protease inhibitor, particularly protecting the lungs from damage by inhibiting enzymes released during inflammatory reactions. Anti-alpha-1 antitrypsin antibodies are specialized proteins that target and bind to AAT, and they have a variety of …

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Serum Free Light Chains Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Serum Free Light Chains (FLC) test measures the levels of free light chains in the blood, which are immunoglobulin light chains not bound to heavy chains. This test has become an essential clinical tool in recent decades for diagnosing and monitoring diseases like …

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The cost of whole-genome sequencing (WGS) for a fungus-Introduction, Keynotes, and Further Readings

The cost of whole-genome sequencing (WGS) for a fungus-Introduction, Keynotes, and Further Readings

Introduction of the cost of whole-genome sequencing (WGS) for a fungus Whole-genome sequencing (WGS) is a powerful technology that has revolutionized the fields of genetics, biology, and medicine. It involves determining the complete DNA sequence of an organism’s genome at a single time. This comprehensive …

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Retrospective versus Prospective Study

Introduction In the realm of research and scientific studies, the terms “retrospective” and “prospective” refer to two different study designs, each with its own methodology and purpose. Differences Here’s a breakdown of the two: Retrospective Study: Prospective Study: Keywords to Understand the Differences

Differences Between Primary, Secondary, Tertiary, and Quaternary Care Centre or Hospital

Introduction In the healthcare system, care is typically structured in a hierarchical model, comprising primary, secondary, tertiary, and quaternary levels. Each level is defined by the complexity of the medical cases they handle, the specialization of medical personnel, and the facilities and equipment available. Differences …

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Ammonia Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction n ammonia blood test is used to measure the level of ammonia in the blood. It is typically performed to detect hyperammonemia, which is a higher than expected level of ammonia​​. Ammonia is produced when proteins are broken down by bacteria in the intestines …

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PPLO Agar- Introduction, Composition, Principle, Preparation Requirements, Test Procedure, Result -Interpretation, Uses, and Keynotes

PPLO Agar- Introduction, Composition, Principle, Preparation Requirements, Test Procedure, Result -Interpretation, Uses, and Keynotes

Introduction PPLO (Pleuropneumonia-Like Organisms) Agar is a specialized growth medium used primarily for the cultivation of Mycoplasma. Mycoplasma species are unique prokaryotes that lack a cell wall and, therefore, have specific nutritional requirements that are provided by the PPLO agar. Components of PPLO Agar: Characteristics …

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ESBL (Extended Spectrum Beta-Lactamase)- Introduction, List of Bacteria, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

ESBL (Extended Spectrum Beta-Lactamase)- Introduction, List of Bacteria, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Extended Spectrum Beta-Lactamase (ESBL) is a term that encompasses a group of enzymes produced by certain bacteria. These enzymes impart resistance to a wide range of beta-lactam antibiotics, which are frequently used in clinical medicine. Here’s an introduction to ESBL: Origin and Mechanism: Clinical …

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Arcanobacterium haemolyticum- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction of Arcanobacterium haemolyticum Arcanobacterium haemolyticum (previously known as Corynebacterium haemolyticum) is a Gram-positive, rod-shaped bacterium. This bacterium is known to be a human pathogen and can cause infections primarily in the pharynx and skin. Here are some key points about this organism: Morphology Arcanobacterium …

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Metallo-beta-lactamases (MBLs)- Introduction, List of Bacteria, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction of Metallo-beta-lactamases (MBLs) Metallo-beta-lactamases (MBLs) are a group of enzymes produced by certain bacteria that impart resistance to a wide range of beta-lactam antibiotics. These enzymes play a crucial role in the global health challenge posed by antibiotic-resistant bacterial infections. Origin and Mechanism: Clinical …

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C-peptide Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction C-peptide, or “connecting peptide,” is a protein fragment that’s a byproduct of insulin production in the pancreas. When proinsulin, a precursor of insulin, is split into its two active components—insulin and C-peptide—both are released into the bloodstream in roughly equal amounts. As a result, …

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Cystatin C Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Cystatin C test is a valuable tool in nephrology and has been growing in importance as an alternative or supplement to traditional kidney function markers. Here’s an introduction to the Cystatin C test: Cystatin C Test Cystatin C is a low molecular weight …

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Cholinesterase Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The cholinesterase test is a significant diagnostic tool in both clinical and occupational medicine. Here’s an introduction to it: Cholinesterase Test: An Introduction Cholinesterase is an enzyme that plays a pivotal role in nerve function in both humans and insects. It’s responsible for breaking …

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Citrobacter farmeri-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Citrobacter farmeri colony morphology on CLED agar

Introduction Citrobacter farmeri is a Gram-negative, facultative anaerobic, rod-shaped bacterium. It belongs to the Enterobacteriaceae family, which also includes other well-known genera like Escherichia, Salmonella, and Klebsiella. The genus “Citrobacter” was named for its ability to utilize citrate as its sole carbon source. Here’s a …

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Prevotella-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Prevotella is a genus of Gram-negative, anaerobic bacteria commonly found in the human oral and gut microbiota. These bacteria have been the focus of numerous studies due to their association with both health and disease in humans. Here’s a brief introduction: Morphology The morphology …

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Troponin I Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Troponin I Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Troponin I Test is a crucial diagnostic tool in cardiology. Here’s an introduction: Troponin I Test: An Overview 1. What is Troponin I? 2. Purpose of the Test: 3. Clinical Utility: 4. Procedure: 5. Interpretation: 6. Advantages Over Other Markers: 7. Considerations: Test …

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Aldolase Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Aldolase Test measures the level of the enzyme aldolase in the blood, which can indicate muscle damage. Here’s a basic outline of the test result, unit, reference range, and test methods: Aldolase Test Test Result, Unit, Reference Range, and Test Methods The Aldolase …

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Homocysteine Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The homocysteine test is an important medical diagnostic tool, especially in the context of cardiovascular health. Here’s an introduction to the topic: What is Homocysteine? Homocysteine is a sulfur-containing amino acid derived from the metabolism of methionine, an essential amino acid obtained from the …

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Next-Generation Sequencing (NGS) Platforms-Introduction, Principle, Types, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction of Next-Generation Sequencing (NGS) Platforms Next-Generation Sequencing (NGS) has transformed the field of genomics and our understanding of the intricacies of the genome. Here’s a brief introduction to the topic: What is Next-Generation Sequencing (NGS)? Next-Generation Sequencing (NGS), also known as high-throughput sequencing, is …

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Haptoglobin Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Haptoglobin is an acute-phase protein synthesized primarily in the liver. Its main physiological function is to bind free hemoglobin (Hb) released from erythrocytes, forming a stable haptoglobin-hemoglobin complex. This prevents the loss of iron and potential kidney damage that could result from hemoglobin passing …

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Restriction fragment length polymorphisms (RFLPs)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction of Restriction fragment length polymorphisms (RFLPs) Restriction Fragment Length Polymorphisms (RFLPs) are a type of genetic variation found within the DNA of organisms, including humans. They were one of the earliest molecular techniques used for genetic analysis and have played a significant role in …

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Arterial Blood Gas (ABG) Analysis: Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Arterial Blood Gas (ABG) Analysis: Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Arterial Blood Gas (ABG) analysis is a medical diagnostic test that measures the acidity (pH) and the levels of oxygen and carbon dioxide in arterial blood. This test provides crucial information about a patient’s respiratory and metabolic status, helping healthcare providers assess the efficiency …

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K. pneumoniae-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

K. pneumoniae-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Klebsiella pneumoniae, often abbreviated as K. pneumoniae, is a species of Gram-negative, rod-shaped bacteria commonly found in the human digestive tract, as well as in soil and water. While it is a part of the normal flora in the human gut, K. pneumoniae can …

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Fructosamine Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The fructosamine test is a laboratory blood test used to monitor and evaluate blood glucose control over a relatively short period, typically spanning two to three weeks. It provides an alternative or complementary measure to the more common hemoglobin A1c (HbA1c) test, which reflects …

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Transferrin saturation (Iron Saturation) Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The transferrin saturation (iron saturation) test is a medical laboratory test used to assess the amount of iron bound to transferrin, a protein that transports iron in the blood. This test provides valuable information about the body’s iron status and is often used in …

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Multilocus sequence typing (MLST)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction Multilocus Sequence Typing (MLST) is a molecular typing technique used in microbiology and genetics to classify and differentiate bacterial strains based on their genetic diversity. MLST is particularly valuable for studying the epidemiology and evolution of bacterial pathogens. Here is an introduction to the …

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Cadmium Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Cadmium Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction A cadmium blood test is a medical diagnostic procedure that measures the concentration of cadmium in a person’s bloodstream. Cadmium is a toxic heavy metal that can be harmful to human health when present in excessive amounts. This test is typically ordered by healthcare …

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Spectrophotometer-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Spectrophotometer-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction A spectrophotometer is a scientific instrument used to measure how different substances absorb or transmit light at various wavelengths. It is a valuable tool in chemistry, biology, physics, and various other scientific disciplines. Spectrophotometry allows researchers to quantitatively analyze the concentration of a solute …

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Zinc Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Zinc Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction A zinc blood test is a medical diagnostic procedure used to measure the concentration of zinc in a person’s bloodstream. Zinc is an essential trace element in the human body and plays a crucial role in various physiological processes. This test helps healthcare providers …

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Pulsed-field gel electrophoresis (PFGE)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Pulsed-field gel electrophoresis (PFGE)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction Pulsed-field gel electrophoresis (PFGE) is a powerful laboratory technique used in molecular biology and genetics to separate and analyze large DNA molecules, such as genomic DNA, by their size. PFGE is particularly valuable when studying organisms with complex genomes, such as bacteria, yeast, and …

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Lead Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Lead Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction A lead blood test, also known as a blood lead level test or BLL test, is a medical diagnostic procedure used to measure the concentration of lead in a person’s bloodstream. Lead is a toxic heavy metal that can have harmful effects on various …

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Tapeworm-Introduction, Morphology, Life Cycle, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Tapeworm-Introduction, Morphology, Life Cycle, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Tapeworms, scientifically known as cestodes, are a type of parasitic flatworm that belong to the class Cestoda. These parasites are characterized by their long, ribbon-like bodies, which are made up of multiple segments called proglottids. Tapeworms are well adapted to a parasitic lifestyle, often …

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Mercury Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Mercury Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction A Mercury Blood Test, also known as a blood mercury level test or serum mercury test, is a medical diagnostic procedure used to measure the concentration of mercury in a person’s bloodstream. Mercury is a heavy metal that can have toxic effects on the …

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Selenium Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction A selenium blood test is a diagnostic test that measures the concentration of selenium in a person’s bloodstream. Selenium is an essential trace element and a micronutrient that plays a crucial role in various physiological processes in the human body. It is obtained through …

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Ascaris lumbricoides-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Adult Ascaris lumbricoides (roundworm)

Introduction Ascaris lumbricoides, commonly known as the “giant roundworm,” is a parasitic nematode (roundworm) that infects the intestines of humans. It is one of the most prevalent and widespread parasitic infections in the world, particularly in areas with poor sanitation and hygiene practices. Here is …

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Lactate Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction A lactate test, also known as a lactic acid test, is a medical diagnostic test that measures the concentration of lactate in the blood. Lactate is a molecule produced when the body breaks down glucose for energy, particularly during periods of intense physical activity …

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Trichuris trichiura-Introduction, Morphology, Life Cycle, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Egg of Trichuris trichura or Whipworm

Introduction Trichuris trichiura, commonly known as the whipworm, is a parasitic nematode (roundworm) that infects the human gastrointestinal tract, primarily the cecum and colon. This parasite is one of the major soil-transmitted helminths (STH) that affect humans worldwide. Here is an introduction to Trichuris trichiura: …

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Radioimmunoassay (RIA)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction of Radioimmunoassay (RIA) Radioimmunoassay (RIA) is a highly sensitive and specific laboratory technique used to measure the concentration of various substances, typically hormones, drugs, or specific proteins, in biological samples such as blood, urine, or tissue extracts. RIA combines principles from both radiochemistry and …

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Brain Natriuretic Peptide (BNP) Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Brain Natriuretic Peptide (BNP) test is a medical diagnostic tool used to assess the functioning of the heart, specifically in the evaluation of heart failure. BNP is a hormone produced by the heart in response to increased pressure and stretching of heart muscle …

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Osteocalcin Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The osteocalcin test, also known as the bone Gla-protein or BGP test, is a medical diagnostic tool used to measure the levels of osteocalcin in the blood. Osteocalcin is a protein primarily produced by osteoblasts, which are cells responsible for bone formation. This test …

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Nephelometry-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Nephelometry-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction Nephelometry is a scientific technique used to measure the scattering of light by particles suspended in a liquid or gas medium. It is an essential tool in various fields, including chemistry, environmental science, and medical diagnostics. The term “nephelometry” is derived from the Greek …

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Micrococcus luteus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Micrococcus luteus colony morphology on Nutrient agar

Introduction Micrococcus luteus, often simply referred to as M. luteus, is a Gram-positive, spherical bacterium belonging to the genus Micrococcus. This bacterium is commonly found in various environments, including soil, dust, water, and on the human skin. Micrococcus species are known for their distinctive yellow …

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Bacterial Vaginosis (BV)-Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Suspected Bacterial Vaginosis Vaginal Swab in Gram Staining Microscopy at a Magnification of 4000X

Introduction Bacterial Vaginosis (BV) is a common vaginal infection characterized by an imbalance in the vaginal microbiota, leading to the overgrowth of certain types of bacteria. It is a condition that primarily affects individuals with female reproductive anatomy and can lead to a range of …

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K. oxytoca-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

K. oxytoca growth on CLED agar

Introduction Klebsiella oxytoca, often abbreviated as K. oxytoca, is a species of gram-negative, rod-shaped bacterium belonging to the Enterobacteriaceae family. This bacterium is closely related to Klebsiella pneumoniae, another well-known member of the same genus. It is a versatile microorganism that can be found in …

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Morganella morganii- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Morganella morganii on CLED agar of Urine Culture

Introduction Morganella morganii is a species of gram-negative bacteria belonging to the family Enterobacteriaceae. This bacterium is named after the American bacteriologist, Thomas J. Morgan. It is commonly found in various environments, including soil, water, and the gastrointestinal tracts of humans and animals. While it …

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Micrococcus roseus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Micrococcus roseus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Micrococcus roseus is a species of bacteria belonging to the genus Micrococcus. This microorganism is known for its distinctive pink to red pigmentation, which is a result of carotenoid pigments present in its cells and it is a Gram-positive, non-motile, and non-spore-forming bacterium. It …

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Respiratory Tract Infections-Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Respiratory Tract Infections-Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Respiratory tract infections (RTIs) are a group of illnesses that affect the respiratory system, which includes the organs and structures involved in breathing. These infections can range from mild to severe and may involve various parts of the respiratory tract, including the upper and …

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Lactobacillus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Lactobacillus and Candida growth on blood agar of curd culture

Introduction Lactobacillus is a genus of bacteria that plays a crucial role in various biological processes, particularly in the fermentation of foods and beverages, as well as in maintaining the balance of the microbiota in the human body. This genus belongs to the lactic acid …

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Dental Caries-Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Dental Caries-Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Dental caries, commonly known as tooth decay or cavities, is a prevalent and multifactorial oral health condition that affects people of all ages worldwide. It is one of the most common chronic diseases, with significant implications for oral and overall health. Dental caries results …

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H9 HbA1C Analyzer (HPLC)-Introduction, Principle, Handling Procedure, Application, and Keynotes

H9 HbA1C Analyzer (HPLC)-Introduction, Principle, Handling Procedure, Application, and Keynotes

Introduction of H9 HbA1C Analyzer The “H9 HbA1c Analyzer (HPLC)” appears to be a medical device used for the measurement of Hemoglobin A1c (HbA1c) levels in blood samples. Let me break down what this means: Here’s how the process typically works: Measuring HbA1c levels is …

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Bacteriophages-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Bacteriophages, often called phages for short, are viruses that specifically infect and replicate within bacteria. They are among the most abundant and diverse biological entities on Earth and play a crucial role in regulating bacterial populations in various ecosystems. Here’s an introduction to bacteriophages: …

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Archaea-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Archaea, pronounced “ahr-kee-uh,” are a fascinating and diverse group of microorganisms that belong to the domain of life known as Archaea. They represent one of the three major domains of life, with the other two being Bacteria and Eukarya. They are distinct from both …

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Candida parapsilosis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Candida parapsilosis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Candida parapsilosis is a species of yeast or fungus that is commonly found in the environment and can also be a commensal organism in the human body. It is a member of the Candida genus, which includes several species of yeast that can cause …

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BCR-ABL1 Genetic Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

BCR-ABL1 Genetic Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Introduction The BCR-ABL1 genetic test is a diagnostic laboratory test that plays a crucial role in the diagnosis, prognosis, and management of certain types of leukemia, most notably chronic myeloid leukemia (CML) and a subset of acute lymphoblastic leukemia (ALL). This test helps identify the …

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Mcfarland Densitometer-Introduction, Principle, Handling Procedure, Application, and Keynotes

Optimal bacterial inoculums (McFarland 0.5 turbidity) for antimicrobial susceptibility testing

Introduction A McFarland densitometer is a simple yet essential laboratory instrument used to measure the optical density of a liquid bacterial suspension. It is a valuable tool in microbiology and clinical laboratories, particularly in tasks like adjusting bacterial cell concentrations for experiments, quality control of …

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E-Test-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Placing Vancomycin E-Test Strip for Staphylococcus aureus

Introduction The E-Test, short for “Epsilometer Test,” is a diagnostic tool used in clinical microbiology and antimicrobial susceptibility testing. It is a method for determining the minimum inhibitory concentration (MIC) of antibiotics or antimicrobial agents against a particular bacterial or fungal pathogen. The E-Test is …

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High-Performance Liquid Chromatography (HPLC)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

High-Performance Liquid Chromatography (HPLC) based Fully Automatic Lifotronic H9 Hemoglobin Analyzer or H9 HBA1C ANALYZER

Introduction of High-Performance Liquid Chromatography (HPLC) High-Performance Liquid Chromatography, commonly known as HPLC, is a powerful analytical technique used in chemistry, biochemistry, and various scientific disciplines for the separation, identification, and quantification of chemical compounds in complex mixtures. It is an essential tool in pharmaceuticals, …

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Micrococcus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Micrococcus luteus growth on Muller-Hinton agar (MHA)

Introduction Micrococcus is a genus of bacteria that belongs to the family Micrococcaceae. These bacteria are Gram-positive, spherical or cocci-shaped, and typically occur in pairs, tetrads, or irregular clusters. Micrococcus species are commonly found in various environments, including soil, water, air, and on the skin …

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Spectrophotometric Assays-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction Spectrophotometric assays are analytical techniques widely used in chemistry, biochemistry, and molecular biology to measure the concentration or absorbance of a substance in a solution. These assays rely on the principle that different molecules absorb light at specific wavelengths, and by measuring the amount …

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Normal Flora-Introduction, Types, Distribution on Human Body, Beneficial Role, Harmful Effects, and Keynotes

Vaginal Cell and Bacterial Interaction in Gram staining of HVS

Introduction Normal flora, also known as the microbiota or microbiome, refers to the diverse and abundant community of microorganisms that reside in and on the human body, as well as in various other organisms and ecological niches. These microorganisms include bacteria, viruses, fungi, and even …

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Staphylococcus haemolyticus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Staphylococcus haemolyticus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Staphylococcus haemolyticus, commonly known as S. haemolyticus, is a species of bacteria belonging to the genus Staphylococcus. It is a Gram-positive, facultative anaerobic coccus (spherical bacterium) and a member of the coagulase-negative staphylococci (CoNS) group. S. haemolyticus is one of several species within the …

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Staphylococcus epidermidis- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Staphylococcus epidermidis Colony Characteristics on Blood agar

 Introduction Staphylococcus epidermidis, often referred to as S. epidermidis, is a type of bacteria that belongs to the Staphylococcus genus. It is commonly found on the skin and mucous membranes of humans and is considered a part of the normal microbiota, which means it is …

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Arsenic Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Arsenic Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction An arsenic blood test is a medical diagnostic procedure used to measure the concentration of arsenic in a person’s bloodstream. Arsenic is a naturally occurring element that can be found in soil, water, and air. While it has some industrial applications, arsenic exposure, particularly …

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Serratia liquefaciens: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Serratia liquefaciens and Klebsiella pneumoniae growth on CLED agar of Urine Culture

Introduction Serratia liquefaciens is a species of Gram-negative, rod-shaped bacteria belonging to the genus Serratia. This bacterium is known for its diverse distribution in various environmental niches, including water, soil, plants, and human-related habitats. It has also been associated with clinical infections in humans, making …

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Ceruloplasmin Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Ceruloplasmin Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Ceruloplasmin Test is a medical diagnostic test used to measure the levels of ceruloplasmin in a person’s blood. Ceruloplasmin is a copper-binding protein that plays a vital role in copper metabolism and transport in the body. This test helps in assessing copper-related disorders …

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Urine Protein electrophoresis Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Urine Protein electrophoresis Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Urine Protein Electrophoresis (UPEP) is a medical diagnostic test used to analyze and separate the proteins found in a urine sample. This test is primarily performed to detect and identify abnormal proteins, particularly those associated with certain medical conditions, including kidney disorders, multiple myeloma, …

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Vitamin D 1,25 Dihydroxy (Calcitriol) Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Vitamin D 1,25-dihydroxy (calcitriol) blood test, also known as the 1,25-dihydroxycholecalciferol test or simply the calcitriol test, is a laboratory examination that measures the concentration of active vitamin D metabolite, calcitriol, in the bloodstream. Calcitriol is the biologically active form of vitamin D …

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Alcohol Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction An alcohol blood test, also known as a blood alcohol test or ethanol blood test, is a medical laboratory test that measures the concentration of alcohol (ethanol) in a person’s bloodstream. This test is commonly used to determine whether an individual has consumed alcohol …

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Lipoprotein (a) Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Lipoprotein(a) blood test, often abbreviated as Lp(a) test, is a diagnostic blood test that measures the concentration of lipoprotein(a) in a person’s bloodstream. Lipoprotein(a) is a unique type of lipoprotein that carries cholesterol through the blood. This test is conducted to assess an …

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Angiotensin-Converting Enzyme (ACE) Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Angiotensin-Converting Enzyme (ACE) blood test is a diagnostic test used to measure the level of ACE enzyme activity in a person’s blood. This test provides valuable information about the activity of ACE in the body, which plays a crucial role in the regulation …

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Adenosine Deaminase (ADA)-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction Adenosine Deaminase (ADA) is an enzyme that plays a critical role in purine metabolism, specifically in the conversion of adenosine to inosine. This enzyme is essential for maintaining the balance of nucleotides in cells and is particularly important for the normal functioning of the …

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Neuron-Specific Enolase (NSE) Blood Test-Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Neuron-Specific Enolase (NSE) Blood Test is a medical diagnostic test used to measure the level of neuron-specific enolase, an enzyme found in neurons, in a patient’s blood. This test is valuable in various clinical settings, particularly in neurology and oncology, and it provides …

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Triglyceride Test- Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction A Triglyceride Test, also known as a triglyceride blood test or lipid panel, is a diagnostic blood test that measures the levels of triglycerides in the bloodstream. Triglycerides are a type of fat (lipid) found in the blood, and they are a key component …

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Blood urea nitrogen (BUN) test- Introduction, Test Result, Unit, Reference Range, Test Methods, Clinical Significance, and Keynotes

Introduction The Blood Urea Nitrogen (BUN) test is a common blood test that measures the level of nitrogen in the form of urea in the blood. Urea is a waste product produced in the liver as a result of the breakdown of proteins and amino …

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Sigmoidoscopy-Introduction, Principle, Handling Procedure, Application, and Keynotes

Introduction Sigmoidoscopy is a medical procedure used to examine the lower part of the large intestine, specifically the sigmoid colon and rectum. It is a type of endoscopy and is performed by a healthcare provider known as a gastroenterologist or colorectal surgeon. Sigmoidoscopy is a …

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Lupus Anticoagulant Profile: Introduction, Components, Clinical Significance, Antiphospholipid Syndrome (APS), Monitoring and Management, Interpretation, and Keynotes

Lupus Anticoagulant Profile Introduction, Components, Clinical Significance, Antiphospholipid Syndrome (APS), Monitoring and Management, Interpretation, and Keynotes

Introduction of Lupus Anticoagulant Profile The Lupus Anticoagulant Panel Test, often referred to as the Lupus Anticoagulant Profile or LA Panel, is a series of laboratory tests used to diagnose and monitor lupus anticoagulant (LA) and other antiphospholipid antibodies (aPL). These tests are essential for …

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Flow Cytometry-Introduction, Principle, Using Procedure, Uses, and Keynotes

Flow Cytometry-Introduction, Principle, Using Procedure, Uses, and Keynotes

Introduction Flow cytometry is a powerful laboratory technique used in the fields of biology, medicine, and immunology to analyze and quantify various physical and chemical characteristics of cells and particles. It allows for the rapid and simultaneous measurement of multiple properties of individual cells as …

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Actinomyces-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Actinomyces-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Actinomyces is a genus of bacteria that includes various species of filamentous, gram-positive, anaerobic or microaerophilic bacteria. These bacteria are known for their ability to form branching, filamentous structures resembling fungi in infected tissues. Actinomyces species are typically found in the normal flora of …

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X-ray-Introduction, Principle, Using Procedure, Uses, and Keynotes

Introduction X-rays, also known as radiographs, are a form of electromagnetic radiation that has been used for over a century in various fields, particularly in medicine and industry, to create images of the inside of objects or the human body. Here’s an introduction to X-rays: …

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Colonoscopy-Introduction, Principle, Using Procedure, Uses, and Keynotes

Introduction Colonoscopy is a medical procedure that involves the examination of the colon (large intestine) and the rectum using a long, flexible tube-like instrument called a colonoscope. This procedure is commonly performed by gastroenterologists and colorectal surgeons and is an important tool in diagnosing, monitoring, …

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Immunofluorescence assay (IFA)-Introduction, Principle, Types, Procedure, Result-Interpretation, Advantage, Disadvantage, and Interfering Factors of ELISA Test Results, and Keynotes

Introduction Immunofluorescence assay (IFA) is a laboratory technique used for detecting and visualizing specific antigens or antibodies in biological samples. It combines the principles of both immunology and fluorescence microscopy to provide highly specific and sensitive results. IFA is widely employed in various fields, including …

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Adenovirus-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Adenoviruses are a group of viruses belonging to the Adenoviridae family. They are named after the tissue (adenoids) where they were first discovered. Adenoviruses are widespread and can infect humans and a variety of animals. In humans, adenoviruses are known to cause a wide …

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PAP Smear-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

PAP Smear-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction A Papanicolaou smear, commonly known as a Pap smear or Pap test, is a routine gynecological screening procedure used to detect abnormal changes in the cells of the cervix, which is the lower part of the uterus (womb) that connects to the vagina. The …

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Cytomegalovirus (CMV)- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Cytomegalovirus (CMV) is a member of the herpesvirus family, specifically Human Herpesvirus 5 (HHV-5), and is a common viral pathogen that infects people of all ages. CMV is a widespread virus, and a majority of adults have been infected with it at some point …

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Stool Occult Blood Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Stool Occult Blood Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Stool Occult Blood Test, also known as the Fecal Occult Blood Test (FOBT) or Hemoccult Test, is a diagnostic test used to detect the presence of hidden (occult) blood in stool samples. This test is valuable for screening and early detection of gastrointestinal …

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Epstein-Barr virus (EBV): Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Epstein-Barr virus (EBV), also known as human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV is known for its ability to establish lifelong infections in humans, primarily in B lymphocytes (a type …

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HBsAg ELISA-Introduction, Test Results, Test Methods, Clinical Significance, and Keynotes

HBsAg ELISA-Introduction, Test Results, Test Methods, Clinical Significance, and Keynotes

Introduction Hepatitis B is a viral infection that can lead to serious liver-related diseases, including cirrhosis and hepatocellular carcinoma. Early detection and diagnosis of Hepatitis B are essential for effective management and prevention of transmission. HBsAg ELISA, an acronym for Hepatitis B Surface Antigen Enzyme-Linked …

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HCV ELISA- Introduction, Test Results, Test Methods, Clinical Significance, and Keynotes

HCV ELISA- Introduction, Test Results, Test Methods, Clinical Significance, and Keynotes

Introduction HCV ELISA stands for Hepatitis C Virus Enzyme-Linked Immunosorbent Assay. It is a laboratory test used to detect antibodies to the Hepatitis C virus (HCV) in a person’s blood. HCV is a bloodborne virus that can cause chronic liver disease, including cirrhosis and liver …

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HIV 1& 2 -Ag & Ab Screening Test- Introduction, Test Results, Test methods, Clinical Significance, and Keynotes

HIV 1& 2 -Ag & Ab Screening Test- Introduction, Test Results, Test methods, Clinical Significance, and Keynotes

Introduction The HIV-1 & 2 -Ag & Ab Screening Test is a crucial diagnostic tool used to detect the presence of antibodies (Ab) and antigens (Ag) associated with the human immunodeficiency virus (HIV). HIV is a virus that attacks the immune system, specifically targeting CD4 …

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HIV, HBsAg and HCV ELISA: Introduction, Test Results, Test methods, Clinical Significance, and Keynotes

HIV, HBsAg and HCV ELISA: Introduction, Test Results, Test methods, Clinical Significance, and Keynotes

Introduction HIV, HBsAg, and HCV ELISA are three distinct diagnostic tests used in the field of medicine and clinical diagnostics to detect specific viral infections. Here’s an introduction to each of these tests: These ELISA tests are essential tools in the diagnosis and monitoring of …

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Soft Tissue Infections- Introduction, Types, Sign and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

 Introduction Soft tissue infections (STIs) refer to a broad category of infections that affect the body’s soft tissues, including muscles, skin, subcutaneous tissue, and connective tissues. These infections can be caused by various pathogens, including bacteria, viruses, fungi, and even parasites. They can range in …

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Scrub Typhus IgM Test: Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Testing Scrub Typhus Antibodies (IgM and IgG) at home

Introduction The Scrub Typhus IgM test is a diagnostic assay used to detect IgM antibodies against the bacteria Orientia tsutsugamushi, which causes scrub typhus, a vector-borne infectious disease. It is prevalent in certain parts of Asia, the Pacific Islands, and other regions with heavy vegetation …

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HIV ELISA: Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

HIV ELISA: Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Introduction HIV ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used laboratory test for the detection of antibodies to the Human Immunodeficiency Virus (HIV) in blood samples. It plays a crucial role in the diagnosis and screening of HIV infection. ELISA is an immunological assay that …

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Gas Gangrene-Introduction, Types, Sign and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Gas gangrene, also known as clostridial myonecrosis, is a severe and potentially life-threatening bacterial infection that primarily affects muscle tissues. It is caused by certain species of bacteria from the Clostridium genus, especially Clostridium perfringens. These bacteria are anaerobic, which means they thrive in …

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SARS-CoV-2 PCR Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

SARS-CoV-2 PCR Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Polymerase Chain Reaction (PCR) test is a molecular diagnostic technique used to detect the genetic material of the SARS-CoV-2 virus in individuals suspected of having COVID-19. This test plays a crucial role in identifying active infections …

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Wound Infections- Introduction, Types, Sign and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Wound infections refer to the invasion and multiplication of pathogenic microorganisms, such as bacteria, in a wound or injury site. These infections can occur after various types of wounds, including surgical incisions, traumatic injuries, burns, and chronic ulcers. Wound infections are a common complication …

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Treponema pallidum haemagglutination (TPHA) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Treponema pallidum haemagglutination (TPHA) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Treponema pallidum haemagglutination (TPHA) test is a diagnostic blood test used to detect antibodies against the bacterium, Treponema pallidum, which is responsible for causing syphilis, a sexually transmitted infection (STI). The TPHA test is a specific and sensitive serological assay that aids in …

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CK-NAC Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The CK-NAC (Creatine Kinase with N-Acetyl Cysteine) test, also known as CK-MB-NAC or CK-NAC enzyme assay, is a diagnostic laboratory test used to measure the level of creatine kinase with N-acetyl cysteine in the blood. Creatine kinase (CK) is an enzyme found primarily in …

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Hepatitis C Antibody (anti-HCV) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Hepatitis C Antibody (anti-HCV) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Hepatitis C Antibody (anti-HCV) test is a crucial diagnostic tool used in the field of medicine to detect the presence of antibodies against the Hepatitis C virus (HCV) in a patient’s blood. HCV is a significant viral infection that can cause chronic liver …

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Salmonella Typhi IgM and IgG Tests-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Salmonella Typhi IgM and IgG Tests-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Introduction Salmonella Typhi IgM and IgG tests are diagnostic tools used to detect antibodies in the blood that are specific to the bacterium Salmonella Typhi. It is the causative agent of typhoid fever, a potentially serious and life-threatening illness. These tests play a crucial role …

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Parathyroid Hormone (PTH) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Parathyroid Hormone (PTH) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Parathyroid Hormone (PTH) test is a diagnostic blood test that measures the level of parathyroid hormone in a person’s bloodstream. This hormone is primarily produced and regulated by the parathyroid glands, which are four small, pea-sized glands located in the neck behind the …

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Uric Acid (UA) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Uric Acid (UA) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Uric Acid (UA) test is a medical diagnostic procedure used to measure the levels of uric acid in a person’s blood or urine. Uric acid is a waste product that is produced when the body breaks down purines, which are chemical compounds found …

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Mantoux Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Mantoux Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Mantoux test, also known as the Tuberculin Skin Test (TST), is a medical diagnostic tool used to determine whether an individual has been exposed to Mycobacterium tuberculosis, the bacterium that causes tuberculosis (TB). This test is named after its creator, Charles Mantoux, a …

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Hepatitis B surface antibody (anti-HBs) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Hepatitis B surface antibody (anti-HBs) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Hepatitis B surface antibody test, also known as the anti-HBs test, is a crucial medical examination used to assess a person’s immunity to the Hepatitis B virus (HBV) or to determine the effectiveness of a Hepatitis B vaccination. This test plays a vital …

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BTCT (Bleeding Time & Clotting Time) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

BTCT (Bleeding Time & Clotting Time) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Bleeding Time and Clotting Time (BTCT) test is a pair of medical diagnostic tests that assess the functioning of blood vessels, platelets, and the blood clotting process. These tests are valuable in evaluating a person’s bleeding and clotting tendencies. Here’s an introduction to …

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Hepatitis B e antigen (HBeAg) Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Hepatitis B e antigen (HBeAg) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Hepatitis B e antigen (HBeAg) test is a medical diagnostic tool used to assess and monitor Hepatitis B virus (HBV) infection. HBV is a contagious virus that attacks the liver and can lead to both acute and chronic liver diseases. The HBeAg test …

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Alpha-Fetoprotein (AFP) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Alpha-Fetoprotein (AFP) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Alpha-Fetoprotein (AFP) test is a blood test commonly used in the field of medicine to measure the levels of alpha-fetoprotein in a person’s bloodstream. Alpha-fetoprotein is a specific protein that is produced by the developing fetus during pregnancy. It plays a vital role …

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Mobiluncus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Mobiluncus is a type of bacteria that can be found in the human vaginal microbiota. It is known for its association with bacterial vaginosis (BV), a common vaginal infection characterized by an imbalance in the vaginal microbiota. BV is not caused by a single …

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Malarial Antigen Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Malarial Antigen Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Malarial Antigen Test, often referred to as the Malaria Rapid Diagnostic Test (RDT) or Malaria Antigen Rapid Test, is a diagnostic tool used to detect the presence of malaria parasites in a patient’s blood. Malaria is a serious and sometimes life-threatening infectious disease …

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Cancer antigen 15-3 (CA15-3) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Cancer antigen 15-3 (CA15-3) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction of Cancer antigen 15-3 (CA15-3) Test The Cancer Antigen 15-3 (CA 15-3) Test is a blood test used in the field of oncology and cancer management to measure the levels of a specific biomarker called CA 15-3 in a patient’s bloodstream. This test is …

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Free Testosterone Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Free Testosterone Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction A Free Testosterone Test, often referred to as a Free T Test, is a medical diagnostic test used to measure the levels of free testosterone in a person’s bloodstream. Testosterone is a hormone primarily produced in the testicles for men and in smaller amounts …

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Hepatitis B surface antigen (HBsAg) Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Hepatitis B surface antigen (HBsAg) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Hepatitis B Surface Antigen (HBsAg) test is a crucial diagnostic tool used in the field of clinical laboratory medicine to detect the presence of the Hepatitis B virus (HBV) in an individual’s bloodstream. Hepatitis B is a viral infection that primarily affects the …

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Testosterone Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Testosterone Test--Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction A testosterone test is a medical procedure used to measure the levels of testosterone, the primary male sex hormone, in a person’s blood. Testosterone plays a crucial role in the development of male reproductive tissues, such as the testes and prostate, and it also …

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Cladosporium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Cladosporium is a genus of fungi that belongs to the phylum Ascomycota. It is a diverse and widespread group of molds that can be found in various environments, including soil, plants, decaying organic matter, and indoor spaces. Cladosporium species (they) are known for their …

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Cancer antigen 125 (CA125) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Cancer antigen 125 (CA125) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Cancer antigen 125 (CA125) test is a blood test that measures the level of a protein called CA125 in the bloodstream. CA125 is a tumor marker, which means that it is a substance produced by certain types of cells, including ovarian cancer cells, …

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Thyroglobulin (Tg) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Thyroglobulin (Tg) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Thyroglobulin (Tg) test is a medical diagnostic tool used to measure the levels of thyroglobulin in a person’s blood. Thyroglobulin is a protein produced by the thyroid gland, a small butterfly-shaped gland located in the front of the neck. The thyroid gland plays …

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Follicle-stimulating Hormone (FSH) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Follicle-stimulating Hormone (FSH) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Follicle-Stimulating Hormone (FSH) test is a medical diagnostic tool used to measure the levels of follicle-stimulating hormone in the blood. FSH is a hormone produced by the pituitary gland, a small gland located at the base of the brain. It plays a crucial …

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Carbamazepine Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Carbamazepine Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Carbamazepine Test is a medical diagnostic procedure used to measure the level of carbamazepine in a person’s blood. Carbamazepine is an anticonvulsant medication primarily prescribed to treat epilepsy, certain types of seizures, and bipolar disorder. It works by stabilizing the electrical activity in …

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Allergy Screening Assay-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Allergy Screening Assay-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction An Allergy Screening Assay is a diagnostic test designed to identify specific allergens that trigger allergic reactions in individuals. Allergies are abnormal immune responses to substances that are usually harmless to most people. These substances, known as allergens, can include pollen, dust mites, pet …

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Procalcitonin (PCT) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Procalcitonin (PCT) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Procalcitonin (PCT) test is a medical diagnostic test used to measure the levels of procalcitonin in the blood. Procalcitonin is a precursor protein to the hormone calcitonin, which is primarily involved in regulating calcium metabolism. However, procalcitonin itself is not a hormone; rather, …

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Rheumatoid Factor (RF) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Rheumatoid Factor (RF) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Rheumatoid Factor (RF) test is a medical diagnostic test used to detect the presence of an antibody called rheumatoid factor in the blood. This antibody is commonly found in the blood of individuals who have rheumatoid arthritis (RA), an autoimmune disorder that primarily …

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Carcinoembryonic antigen (CEA) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Carcinoembryonic antigen (CEA) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Carcinoembryonic Antigen (CEA) test is a medical laboratory test used to measure the levels of a protein called carcinoembryonic antigen in the blood. CEA is a glycoprotein that is normally produced during fetal development, but its production decreases significantly after birth. It is …

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Tacrolimus Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Tacrolimus Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Tacrolimus test, also known as a Tacrolimus blood test or Tacrolimus level test, is a medical diagnostic procedure used to measure the concentration of tacrolimus in a person’s blood. Tacrolimus is a potent immunosuppressive medication commonly prescribed to organ transplant recipients to prevent …

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Valproic Acid Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Valproic Acid Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Valproic Acid Test is a medical diagnostic procedure used to measure the level of valproic acid in a person’s blood. Valproic acid is a medication primarily prescribed to treat certain seizure disorders, such as epilepsy, as well as bipolar disorder and migraines. It …

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Stenotrophomonas maltophilia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Mucoid lactose fermenting (MLF) colony of Klebsiella pneumoniae and non-lactose fermenting (NLF) colony of Stenotrophomonas maltophilia on MacConkey medium of clinical specimen-sputum culture

Introduction Stenotrophomonas maltophilia is a Gram-negative bacterium that belongs to the class Gammaproteobacteria. It is an opportunistic pathogen known for its ability to cause infections, particularly in individuals with compromised immune systems or underlying health conditions. The bacterium was initially considered to be of low …

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Estradiol (E2) Test: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Estradiol (E2) Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Estradiol (E2) test is a medical diagnostic procedure that measures the levels of estradiol in the blood. Estradiol is the primary and most potent form of estrogen, a hormone that plays a crucial role in various physiological processes, particularly those related to the …

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Bacteroides: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Bacteroides is a genus of bacteria that belong to the Bacteroidaceae family. These bacteria are anaerobic, which means they thrive in environments with little to no oxygen. Bacteroides species (they) are commonly found in the gastrointestinal tract of humans and animals, where they play …

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Anti-CCP Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Anti-CCP Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Anti-CCP (Anti-Cyclic Citrullinated Peptide) test is a diagnostic tool used in the field of rheumatology and autoimmune diseases. It is primarily used to assist in the diagnosis of rheumatoid arthritis (RA), an autoimmune disorder that primarily affects the joints but can also have …

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Serum Cortisol Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Serum Cortisol Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Serum Cortisol Test is a medical diagnostic tool used to measure the level of cortisol in the bloodstream. Cortisol is a steroid hormone produced by the adrenal glands, which are located on top of the kidneys. It plays a crucial role in various …

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Lithium Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Lithium Test- Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction In the medical field, a lithium blood test is used to measure the concentration of lithium in a person’s blood. Lithium is commonly prescribed as a mood stabilizer for individuals with bipolar disorder. Monitoring the levels of lithium in the blood is essential to …

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Prolactin (PRL) Test: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Prolactin (PRL) Test Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Prolactin (PRL) test is a medical diagnostic test used to measure the levels of prolactin, a hormone produced by the pituitary gland in the brain. Prolactin plays a vital role in various physiological processes, primarily associated with reproductive health and lactation (milk production). …

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Fonsecaea: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Fonsecaea: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Fonsecaea is a genus of fungi commonly associated with human and animal infections. It belongs to the class Eurotiomycetes and the order Chaetothyriales. The most clinically relevant species within this genus is Fonsecaea pedrosoi, which is known to cause chromoblastomycosis, a chronic and localized …

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Luteinizing Hormone (LH) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Luteinizing Hormone (LH) Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Luteinizing Hormone (LH) test is a medical diagnostic tool used to measure the levels of luteinizing hormone in a person’s blood or urine. LH is a hormone produced by the anterior pituitary gland in the brain. It plays a crucial role in regulating …

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Peritoneal Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Peritoneal fluid culture and sensitivity is a medical diagnostic procedure used to identify and determine the susceptibility of microorganisms present in the peritoneal fluid. Peritoneal fluid is a sterile fluid that fills the peritoneal cavity, which is the space within the abdomen that surrounds …

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DHEA Sulfate Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

DHEA Sulfate Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The DHEA sulfate test, also known as the dehydroepiandrosterone sulfate test, is a medical diagnostic procedure that measures the levels of DHEA sulfate (DHEA-S) in the blood. DHEA-S is a steroid hormone produced primarily by the adrenal glands, which are located on top of …

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Ascitic Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Ascitic fluid culture and sensitivity is a medical diagnostic procedure used to identify and analyze bacterial or fungal infections within the peritoneal cavity. The peritoneal cavity is the space within the abdomen that contains various organs such as the liver, intestines, and spleen. Ascitic …

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Clinical Laboratory Gallery: Introduction, Contents, and Brief Description of Photos

Wet Mount Microscopy-Ready slide for observation

Introduction ‘Clinical Laboratory Gallery’ is a collection of genuine photos regarding stream of Clinical Laboratory like Stool and Urine Section (SUS), Phlebotomy, Clinical Haematology, Clinical Biochemistry, Blood Banking and Transfusion medicine, Microbiology and Immunology, Cytology and Histopathology, and Molecular Biology. Contents Collection of images are …

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Insulin Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Insulin-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction An insulin test is a medical procedure used to measure the level of insulin in a person’s blood. Insulin is a hormone produced by the pancreas, a gland located behind the stomach. It plays a crucial role in regulating blood sugar (glucose) levels and …

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Skin and Soft Tissue Infections: Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Skin and soft tissue infections (SSTIs) encompass a broad range of infections that affect the skin, underlying connective tissues, and sometimes even the muscles. These infections can be caused by various types of bacteria, viruses, fungi, and parasites, and they can occur in individuals …

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Otitis Media: Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Otitis media is a medical term used to describe inflammation and infection of the middle ear. The middle ear is the space behind the eardrum that contains the small bones responsible for transmitting sound vibrations to the inner ear. It is a common condition, …

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Darkfield Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Introduction A darkfield microscope is a specialized optical instrument used in microscopy to visualize transparent specimens that are otherwise difficult to see using traditional brightfield illumination. It operates on the principle of illuminating the sample with oblique or angled light, causing the specimen to appear …

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Influenza Viruses: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Influenza viruses, commonly known as the flu viruses, are a group of RNA viruses belonging to the Orthomyxoviridae family. These viruses are responsible for causing influenza, a highly contagious respiratory illness that affects humans, birds, and some other animals. Influenza viruses are known for …

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Food Poisoning:  Introduction, Types, Signs and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Food poisoning, also known as foodborne illness, is a widespread and potentially serious public health concern that occurs when individuals consume contaminated or spoiled food. It is caused by the ingestion of harmful microorganisms such as bacteria, viruses, parasites, or their toxins, which can …

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Stool Routine Examination: Introduction, Common Test, Test Report, Clinical Significance, and Keynotes

A step of Stool Routine Examination procedure

Introduction Stool routine examination, also known as a fecal routine or stool analysis, is a common medical test used to assess the health and functioning of the gastrointestinal tract. It involves the collection and analysis of a stool sample to provide valuable insights into various …

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Electron Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Electron Microscope Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Introduction An electron microscope is a powerful scientific instrument used to observe extremely small objects, structures, and details at a level of magnification that is not achievable with traditional light microscopes. It utilizes a beam of accelerated electrons instead of visible light to create high-resolution …

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Tuberculosis: Introduction, Types, Sign and Symptoms, Causative Agent, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Tuberculosis (TB) is a contagious bacterial infection that primarily affects the lungs, although it can also impact other parts of the body. It is caused by Mycobacterium tuberculosis, a type of bacteria that spreads through the air when an infected person coughs, sneezes, or …

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Bloodstream Infections(BSI): Introduction, Types, Sign and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Bloodstream infections (BSI), also known as bacteremia or septicemia, are serious medical conditions characterized by the presence of bacteria or other pathogens in the bloodstream. This condition can lead to severe health complications and even be life-threatening if not promptly diagnosed and treated. Bloodstream …

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Pneumonia: Introduction, Types, Sign and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Pneumonia is a common and potentially serious respiratory infection that affects the lungs. It can be caused by various infectious agents, including bacteria, viruses, fungi, and other microorganisms. The infection causes inflammation and swelling in the air sacs of the lungs, known as alveoli, …

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VDRL Test: Introduction, Test Result, Normal Range, Test method, Clinical Significance, and Keynotes

Introduction The Venereal Disease Research Laboratory (VDRL) test is a blood test used to detect the presence of antibodies that are produced by the body in response to certain infections, primarily syphilis. Syphilis is a sexually transmitted infection (STI) caused by the bacterium Treponema pallidum. …

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Prothrombin Time Test and INR (PT/INR): Introduction, Test Result, Normal Range, Test method, Clinical Significance, and Keynotes

Prothrombin Time Test and INR (PT/INR)

Introduction The Prothrombin Time Test (PT) and International Normalized Ratio (INR) are crucial medical tests used to assess the clotting ability of blood. These tests play a vital role in monitoring patients on anticoagulant medications, evaluating bleeding disorders, and assessing liver function. Prothrombin Time Test …

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Ureaplasma: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Ureaplasma is a genus of bacteria that belong to the class Mollicutes. These bacteria are characterized by their small size, lack of a cell wall, and a simple structure. Ureaplasma species (they) are known to colonize the human urogenital tract and are associated with …

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Legionella pneumophila: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Legionella pneumophila is a gram-negative, aerobic bacterium responsible for causing Legionnaires’ disease, a severe form of pneumonia, and Pontiac fever, a milder flu-like illness. It was first identified in 1976 when an outbreak of pneumonia affected attendees of an American Legion convention in Philadelphia, …

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Microsporum ferrugineum: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Microsporum ferrugineum in LPCB taese mount of culture microscopy showing typical bamboo hyphae and lacking production of both microconidia and macroconidia

Introduction Microsporum ferrugineum is a species of dermatophytic fungus that belongs to the genus Microsporum. Dermatophytes are a group of fungi that are capable of infecting and colonizing the outer layers of the skin, hair, and nails, causing various dermatophytosis or “ringworm” infections in humans …

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Inverted Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Inverted Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Introduction An inverted microscope is a specialized optical instrument that revolutionizes the way we observe and study biological specimens, live cells, and other materials in a liquid medium. Unlike conventional microscopes, where the objective lens is above the specimen, the inverted microscope ingeniously flips this …

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Mycoplasma pneumoniae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Mycoplasma pneumoniae is a unique type of bacteria that belongs to the class Mollicutes. What sets it apart from other bacteria is its lack of a cell wall, which is a characteristic feature of most bacterial species. This absence of a cell wall makes …

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Geotrichum: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Geotrichum candidum colony morphology on Sabouraud dextrose agar (SDA) in clinical specimen, pus

Introduction Geotrichum is a genus of fungi that belong to the family Geotrichaceae. These fungi are commonly found in various environmental settings, including soil, water, and decaying organic matter. Geotrichum species can also be found as part of the natural microbiota in humans and animals. …

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Fluorescence Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Fluorescence Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Introduction A Fluorescence Microscope is a powerful optical instrument used to observe and study specimens that emit fluorescence when exposed to specific wavelengths of light. This technique exploits the unique properties of fluorescence, where certain molecules or structures absorb light at one wavelength and emit …

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Phase Contrast Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Phase Contrast Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Introduction A Phase Contrast Microscope is an optical instrument designed to enhance the visibility of transparent and unstained samples, such as living cells, by converting phase differences in light waves into brightness variations in the image. This technique was invented by Dutch physicist Frits Zernike …

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E. aerogenes: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Enterobacter aerogenes colony morphology on CLED agar of urine culture

Introduction Enterobacter aerogenes is a bacterium belonging to the Enterobacteriaceae family, which includes several other important pathogens and opportunistic bacteria. E. aerogenes is a Gram-negative, rod-shaped bacterium that is commonly found in various environments, including soil, water, and the gastrointestinal tracts of humans and animals. …

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Enteroviruses: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Enteroviruses are a group of viruses that belong to the Picornaviridae family. They are a diverse group of viruses that can cause a wide range of illnesses, from mild cold-like symptoms to more serious diseases. Enteroviruses primarily infect the gastrointestinal tract and can spread …

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MALDI-TOF Mass Spectrometry: Introduction, Principle, Handling Procedure, Application, and Keynotes

Introduction Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, commonly abbreviated as MALDI-TOF MS, is a powerful analytical technique used in the field of mass spectrometry. It is widely employed for the identification and characterization of a diverse range of molecules, including proteins, peptides, nucleic acids, carbohydrates, …

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Pleural Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Pleural fluid for laboratory diagnosis

Introduction Pleural fluid culture and sensitivity is a diagnostic test used to identify and determine the antibiotic susceptibility of microorganisms present in the pleural fluid. The pleural fluid is a clear fluid that accumulates between the two layers of the pleura, the membrane that surrounds …

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Shewanella: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Shewanella species colony characteristics on Muller-Hinton agar (MHA)

Introduction Shewanella is a genus of Gram-negative, facultative anaerobic bacteria that belongs to the family Shewanellaceae. These bacteria are known for their remarkable versatility and adaptability to a wide range of environmental conditions. They were first discovered in 1980 by researchers studying microbial fuel cells, …

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Clostridium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Clostridium growth on blood agar under anaerobic condition of 48 hours- inhibition of growth around metronidazole but no effect towards gentamicin

Introduction Clostridium is a genus of Gram-positive, rod-shaped, spore-forming bacteria that belongs to the phylum Firmicutes. These bacteria are anaerobic, meaning they can thrive in environments devoid of oxygen. The name “Clostridium” is derived from the Greek word “kloster,” which means spindle, referring to their …

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Moraxella catarrhalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Gram negative cocci in pairs (diplococci) of Moraxella catarrhalis in Gram staining of culture microscopy at a magnification

Introduction Moraxella catarrhalis is a Gram-negative, aerobic bacterium that belongs to the Moraxellaceae family. It is a non-motile, non-spore-forming bacterium, and it is typically found in the upper respiratory tract of humans as a commensal organism. Though generally considered a commensal, M. catarrhalis can also …

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Gardnerella vaginalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Gardnerella vaginalis is a Gram-variable bacterium that is considered to be a facultative anaerobe, meaning it can survive in both aerobic and anaerobic environments. It is the main causative agent of bacterial vaginosis (BV), a common vaginal infection in women of reproductive age. BV …

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Chlamydia pneumoniae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Chlamydia pneumoniae, also known as Chlamydophila pneumoniae, is a species of bacteria that belongs to the Chlamydiaceae family. It is an obligate intracellular pathogen, meaning it can only survive and replicate inside the cells of its host. It primarily infects the respiratory system, causing …

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Thorat Swab Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Pin point beta-haemolytic colony of Streptococcus pyogenes (group A streptococci) on blood agar of throat swab culture

Introduction of Thorat Swab Culture and Sensitivity Thorat swab culture and sensitivity is a medical diagnostic procedure used to identify the causative agents of infections in the throat and determine their sensitivity to various antibiotics. It is commonly performed when a patient presents with symptoms …

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Pericardial Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Pericardial fluid culture and sensitivity is a diagnostic test performed to identify and determine the antibiotic susceptibility of microorganisms present in the pericardial fluid. The pericardium is a protective membrane that surrounds the heart, and the pericardial fluid is a small amount of fluid …

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Staphylococcus saprophyticus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Staphylococcus saprophyticus on Muller-Hinton agar (MHA) showing novobiocin resistance

Introduction Staphylococcus saprophyticus is a species of bacteria that belongs to the genus Staphylococcus. It is a Gram-positive, coagulase-negative, and facultative anaerobic bacterium. Like other staphylococci, S. saprophyticus typically appears in clusters under a microscope, which is characteristic of its name, derived from the Greek …

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Mycobacterium leprae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Heavy load of acid fast bacilli (AFB) of Mycobacterium leprae in modified Ziehl-Neelsen staining of slit skin smear microscopy at a magnification of 1000X

Introduction Mycobacterium leprae is the bacterium responsible for causing leprosy, also known as Hansen’s disease. It is an ancient disease that has afflicted humans for thousands of years, and it continues to be a significant public health concern in some parts of the world. M. …

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Herpes Simplex Virus (HSV): Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Tzanck Smear- Positive of Herpes Simplex Virus (HSV)

Introduction Herpes Simplex Virus (HSV) is a common and highly contagious virus that infects humans. It belongs to the family Herpesviridae and is characterized by its ability to establish a lifelong infection in the host. HSV exists in two main types: HSV-1 and HSV-2. Transmission: …

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Mycobacterium tuberculosis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Mycobacterium tuberculosis colony morphology on LJ medium

Introduction Mycobacterium tuberculosis is a bacterium that causes tuberculosis (TB), a contagious infectious disease primarily affecting the lungs but can also affect other parts of the body. TB has been a significant public health concern worldwide, causing illness and death for thousands of years. Key …

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Polymerase Chain Reaction (PCR): Introduction, Principle, Types, Application, and Keynotes

Amplicons or DNA or RNA fragments of amplification or replication events

Introduction Polymerase Chain Reaction, commonly known as PCR, is a powerful and widely used molecular biology technique that allows for the amplification of specific DNA sequences. Developed by Kary B. Mullis in 1983, PCR revolutionized the field of genetics and has become an essential tool …

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Achromobacter xylosoxidans: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Gram negative rods (GNRs) or Gram negative bacilli (GNBs)of Achromobacter xylosoxidans in Gram staining of culture microscopy

Introduction Achromobacter xylosoxidans is a gram-negative, non-fermenting bacterium that belongs to the family Alcaligenaceae. It is an aerobic, rod-shaped bacterium known for its versatile metabolic capabilities and its widespread distribution in various environments, including soil, water, and hospital settings. Originally classified as Alcaligenes xylosoxidans, the …

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Cerebrospinal fluid (CSF) Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Aspirated Cerebrospinal fluid (CSF) for Culture -Sensitivity, and more

Introduction Cerebrospinal fluid (CSF) culture and sensitivity is a medical laboratory test performed to detect and identify any infectious organisms that may be present in the cerebrospinal fluid. The CSF is a clear and colorless fluid that surrounds the brain and spinal cord, providing essential …

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Streptococcus pneumoniae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Optochin sensitive Streptococcus pneumoniae

Introduction Streptococcus pneumoniae, commonly known as pneumococcus, is a bacterium that can cause a wide range of infections in humans. It is one of the most significant pathogens responsible for various diseases, particularly pneumonia, meningitis, and otitis media (middle ear infections). Characteristics: Transmission: Diseases caused …

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Varicella-Zoster Antibody, IgG Assay:Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Varicella-Zoster Antibody, IgG Assay:Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction Varicella-zoster antibody, IgG assay, is a medical test used to measure the levels of varicella-zoster virus (VZV) specific immunoglobulin G (IgG) antibodies in the blood. VZV is the virus responsible for causing chickenpox (varicella) and can also re-emerge later in life as shingles (herpes …

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Enterococcus faecium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Gram positive cocci in singles, pairs and chains of Enterococcus faecium in Gram staining of culture microscopy

Introduction Enterococcus faecium is a bacterium that belongs to the Enterococcus genus, which is a part of the Firmicutes phylum. It is a Gram-positive, facultative anaerobic coccus, typically occurring in pairs or short chains. Enterococci are commensal bacteria commonly found in the gastrointestinal tract of …

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Sputum Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

A step of sample inoculation on blood agar for Sputum Culture and Sensitivity

Introduction Sputum culture and sensitivity is a medical laboratory test used to identify and determine the susceptibility of bacteria or other microorganisms present in a patient’s sputum, a mixture of saliva and mucus coughed up from the respiratory tract. This diagnostic test is crucial in …

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Pus Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Pus culture and sensitivity is a diagnostic test commonly performed in medical settings to identify and analyze the microorganisms present in a wound, abscess, or other sources of pus. Pus is a thick, yellowish or greenish fluid that accumulates at the site of infection …

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Trichomonas vaginalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Trichomonas vaginalis is a parasitic protozoan that causes the sexually transmitted infection (STI) known as trichomoniasis. This unicellular organism primarily infects the urogenital tract in both men and women. Trichomoniasis is one of the most common curable STIs worldwide. Here are some key points …

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High Vaginal Swab (HVS) Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction High Vaginal Swab (HVS) Culture and Sensitivity is a diagnostic test used to identify and analyze the microorganisms present in the vaginal region. It is an important tool in gynecology and obstetrics to diagnose and manage various vaginal infections, such as bacterial vaginosis, yeast …

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Streptococcus agalactiae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Beta-haemolytic colony of Streptococcus agalactiae on 5% sheep blood agar of clinical specimen, High Vaginal Swab (HVS)

Introduction Streptococcus agalactiae, commonly known as Group B Streptococcus (GBS), is a Gram-positive bacterium belonging to the Streptococcaceae family. It is a significant human pathogen known for causing various infections in both adults and newborns. The bacterium is typically found as a part of the …

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Urine Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Urine routine examination showing numerous pus cells and bacteria -urinary tract infection (UTI) due to bacteria

Introduction Urine culture and sensitivity is a medical diagnostic test used to identify and quantify the microorganisms present in a urine sample and determine their sensitivity to various antibiotics. It is a crucial procedure in diagnosing urinary tract infections (UTIs) and guiding appropriate treatment. Here’s …

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Streptococcus pyogenes: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Streptococcus pyogenes colony morphology on blood agar

Introduction Streptococcus pyogenes, also known as Group A Streptococcus (GAS), is a gram-positive, spherical bacterium that belongs to the Streptococcaceae family. It is one of the most common and important human pathogens, responsible for a wide range of infections in humans. This bacterium is typically …

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Chlamydia trachomatis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Chlamydia trachomatis is a gram-negative bacterium and is an important human pathogen known for causing several diseases. It is an obligate intracellular bacterium, meaning it can only survive and reproduce inside host cells. C. trachomatis primarily infects epithelial cells of the mucous membranes in …

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Corynebacterium diphtheriae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Corynebacterium diphtheriae colony morpholoy on 5% sheep blood agar after 48 hours of incubation

Introduction Corynebacterium diphtheriae is a gram-positive, rod-shaped bacterium that causes diphtheria, a highly contagious and potentially life-threatening respiratory infection. This bacterium was first identified and described by the German physician Edwin Klebs in 1883, and later confirmed by Friedrich Loeffler and Emil von Behring. The …

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Mycoplasma hominis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Mycoplasma hominis is a species of bacteria belonging to the Mollicutes class. It is a unique microorganism as it lacks a cell wall, making it one of the smallest self-replicating organisms known. Due to this feature, it has a pleomorphic appearance, taking various shapes, …

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Anti-Thyroid Peroxidase (Anti-TPO) Assay: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Anti-Thyroid Peroxidase (Anti-TPO) Assay: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Anti-Thyroid Peroxidase (Anti-TPO) assay is a diagnostic test used to measure the levels of anti-thyroid peroxidase antibodies in a person’s blood. Thyroid peroxidase (TPO) is an enzyme found in the thyroid gland, which is responsible for the production of thyroid hormones, such as …

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Urinalysis: Introduction, List of Some Common Tests, Test Report, Clinical Significance, and Keynotes

Urinalysis-Introduction, List of Some Common Tests, Test Report, Clinical Significance, and Keynotes

Introduction Urinalysis is a diagnostic medical test that involves the examination of urine to evaluate a person’s health and detect various medical conditions. It is one of the most commonly performed laboratory tests and provides valuable information about the body’s metabolism, kidney function, and overall …

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Escherichia coli: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Greenish metallic sheen of Escherichia coli on Eosin Methylene Blue (EMB) Agar

Introduction Escherichia coli, often abbreviated as E. coli, is a gram-negative, rod-shaped bacterium belonging to the Enterobacteriaceae family. It is one of the most commonly studied and well-known bacteria, both because of its importance in scientific research and its relevance in various aspects of human …

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Citrobacter koseri: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Mucoid colony of Citrobacter koseri on CLED agar of urine specimen

Introduction Citrobacter koseri, formerly known as Citrobacter diversus, is a gram-negative bacterium belonging to the Enterobacteriaceae family. It is a facultative anaerobe, meaning it can grow both in the presence and absence of oxygen. The species name “koseri” is a tribute to the bacteriologist Leo …

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Scabies: Introduction, Sign and Symptoms, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Scabies is a common and contagious skin infestation caused by the microscopic mite Sarcoptes scabiei. It is prevalent worldwide and can affect people of all ages and socioeconomic backgrounds. Scabies mites burrow into the top layer of the skin, where they lay eggs and …

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Compound Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Compound Microscope- Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes_

Introduction The compound microscope is a fundamental and widely used optical instrument that allows us to magnify and observe small objects or specimens that are otherwise invisible to the naked eye. It is an essential tool in various scientific fields, including biology, medicine, microbiology, and …

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Simple Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Introduction A simple microscope is a basic optical instrument used for magnifying small objects or specimens that are not visible to the naked eye. It consists of a single lens that can be either convex or concave, mounted in a frame with a handle or …

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Trichomonas vaginalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Trichomonas vaginalis is a parasitic protozoan that primarily infects the urogenital tract of humans, causing the sexually transmitted infection (STI) known as trichomoniasis. This microorganism is responsible for one of the most common curable STIs worldwide. T. vaginalis was first identified by Alfred Francois …

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Semen Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Semen Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Semen culture and sensitivity is a medical test used to evaluate the presence of microorganisms in a man’s semen (ejaculate) and determine their susceptibility to various antibiotics. It is an essential diagnostic tool used to investigate male reproductive health issues, particularly when there are …

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Streptococcus agalactiae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a Gram-positive bacterium that belongs to the Streptococcaceae family. It is a significant human pathogen and is commonly found in the gastrointestinal and genitourinary tracts of healthy adults. While it may not cause any …

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Folic Acid Test: Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance, and Keynotes

Folic Acid Test Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance, and Keynotes

Introduction Folic acid, also known as vitamin B9 or folate, is an essential nutrient required for various bodily functions, including cell division, DNA synthesis, and red blood cell formation. Adequate levels of folic acid are particularly crucial during periods of rapid growth, such as pregnancy, …

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Total Iron-Binding Capacity (TIBC) Test: Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance, and Keynotes

Total Iron-Binding Capacity (TIBC) Test Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance, and Keynotes

Introduction The Total Iron-Binding Capacity (TIBC) test is a medical diagnostic tool used to assess the body’s capacity to bind and transport iron in the blood. Iron is an essential mineral required for various physiological processes, including oxygen transport and energy production. The TIBC test …

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Nasal Swab Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Nasal Swab Culture and Sensitivity Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Nasal swab culture and sensitivity is a diagnostic laboratory test used to identify and determine the antibiotic sensitivity of microorganisms present in the nasal passages. It is a valuable tool in the diagnosis and management of various respiratory infections, particularly those caused by bacteria. …

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Eye Flu: Introduction,Causes, Symptoms, Effective Treatment, Prevention and Keynotes

Introduction Eye flu, also known as conjunctivitis, is a common and highly contagious eye infection that affects the conjunctiva, the thin and transparent layer covering the white part of the eye and the inner surface of the eyelids. It is characterized by redness, irritation, and …

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Malassezia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Malassezia is a genus of yeast-like fungi that belongs to the class Malasseziomycetes. These fungi are part of the normal skin microbiota of humans and other animals, commonly found on the skin surfaces, particularly in sebum-rich areas such as the scalp, face, chest, and …

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Bipolaris: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Bipolaris is a genus of filamentous fungi belonging to the family Pleosporaceae within the order Pleosporales. These fungi are commonly found in various environments, such as soil, plant debris, and decaying vegetation. Bipolaris species (they) are known to be plant pathogens, causing a range …

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Epidermophyton floccosum: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Epidermophyton floccosum is a dermatophyte fungus belonging to the group of fungi responsible for causing superficial fungal infections in humans and animals. Dermatophytes are known for their ability to infect the skin, hair, and nails, causing conditions collectively referred to as dermatophytosis or ringworm …

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Sporothrix schenckii: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Sporothrix schenckii colony morphology on SDA

Introduction Sporothrix schenckii is a dimorphic fungus and the causative agent of the infection known as sporotrichosis or “rose gardener’s disease.” It is a relatively common fungal pathogen found worldwide, and it primarily affects the skin and subcutaneous tissues, although it can occasionally spread to …

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Trichophyton mentagrophytes: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Colony morphology of Trichophyton mentagrophytes on dermatophyte test medium (DTM) plate and tube

Introduction Trichophyton mentagrophytes is a dermatophyte fungus belonging to the genus Trichophyton, which is known for causing various skin infections in humans and animals. It is an anthropophilic fungus, meaning it primarily infects humans, but it can also infect animals like rodents and domesticated pets. …

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Penicillium marneffei: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Conidia, conidiophore, phialide,and metullae of Penicillium marneffei in LPCB tease mount of culture microscopy at a magnification of 400X

Introduction Penicillium marneffei is a thermally dimorphic fungus and a significant human pathogen, causing a rare but severe disease called penicilliosis marneffei. It is classified under the phylum Ascomycota and the family Talaromycetaceae. The fungus was first discovered and described by a French physician, Captain …

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Ochroconis gallopava: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Conidiophores and conidia of Ochroconis gallopava in LPCB tease mount of culture microscopy

Introduction Ochroconis gallopava is a species of fungus belonging to the Ascomycota phylum. It is commonly known as the “riverbank grape black rot fungus.” This fungus is primarily known for its pathogenic properties, as it can cause significant damage to various plant species, particularly grapevines. …

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Scopulariopsis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Chains of single-celled conidia and conidiophores(annellides) of Scopulariopsis in LPCB Tease mount of culture microscopy

Introduction Scopulariopsis is a genus of filamentous fungi belonging to the class Ascomycota. It is a widespread and diverse group of fungi found in various environmental niches. Scopulariopsis species (they) are commonly present in soil, decaying organic matter, and indoor environments. The name “Scopulariopsis” is …

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Trichoderma: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Conidia, phialides and conidiophores of Trichoderma in LPCB tease mount of culture microscopy

Introduction Trichoderma is a genus of fungi that plays a crucial role in agriculture, biotechnology, and environmental management. It is part of the phylum Ascomycota and is known for its diverse species that have the ability to interact with plants, other fungi, and soil microorganisms. …

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Fusarium oxysporum: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Fusarium oxysporum growth on SDA tube

Introduction Fusarium oxysporum is a filamentous fungus belonging to the genus Fusarium. It is a widespread and economically significant plant pathogen that affects a wide range of crops, causing various diseases collectively known as “Fusarium wilt” or “Fusarium yellows.” This pathogen can infect over 100 …

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Salmonella Agglutination Test: Introduction, Test Result, Test Method, Clinical Significane, and Keynotes

Salmonella Agglutination Test

Introduction The Salmonella agglutination test is a laboratory diagnostic method used to identify and serotype Salmonella based on their surface antigens. Salmonella is a genus of bacteria that includes various species, some of which can cause foodborne illnesses and gastroenteritis in humans and animals. The …

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Cryptococcus neoformans: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Abundant Yeast cells with occasional hyphae of Cryptococcus neoformans in India ink preparation of culture microscopy

Introduction Cryptococcus neoformans is a pathogenic fungus and one of the leading causes of fungal infections in humans. It belongs to the genus Cryptococcus, which includes several species, but C. neoformans is the most clinically significant one. This fungus primarily affects immunocompromised individuals, such as …

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Syncephalastrum: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Syncephalastrum is a genus of fungi belonging to the order Mucorales. It is characterized by its unique morphology and life cycle, which differentiates it from other fungal groups. Syncephalastrum species are commonly found in soil and decaying organic matter, where they play essential roles …

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Cylindrocarpon : Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Cylindrocarpon is a genus of fungi that belongs to the class Dothideomycetes. This genus is characterized by its cylindrical or slightly curved, hyaline (colorless) conidia, which are the asexual spores produced by the fungus. The name “Cylindrocarpon” is derived from Greek, where “cylindro” means …

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Fusarium solani: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Macroconidia and conidiophores of Fusarium solani in LPCB tease mount of culture

Introduction Fusarium solani is a filamentous fungus belonging to the genus Fusarium. It is commonly found in the soil and is recognized as one of the most versatile and widespread species within the Fusarium genus. This fungus is known for its ecological importance, as it …

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Curvularia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Curvularia Colony characteristics on SDA-Reverse side view

Introduction Curvularia is a genus of filamentous fungi belonging to the family Pleosporaceae, commonly found in soil, decaying plant material, and various environmental niches. These fungi are known for their distinctive curved or sigmoid-shaped conidia (asexual spores), which give the genus its name. Characteristics: Pathogenicity: …

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Leptospira IgM and IgG Test: Introduction, Test Result, Test Method, Clinical Significane, and Keynotes

Leptospira IgM and IgG Test Result-Negative

Introduction The Leptospira IgM and IgG test is a serological diagnostic test used to detect specific antibodies against Leptospira bacteria in a person’s blood. Leptospirosis is a zoonotic disease caused by infection with pathogenic Leptospira species, and it can affect both animals and humans. The …

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Brucella Antibody Test: Introduction, Test Result, Test Method, Clinical Significane, and Keynotes

Brucella Agglutination Test

Introduction The Brucella antibody test is a diagnostic test used to detect antibodies against Brucella bacteria in a person’s blood. Brucella species are the causative agents of brucellosis, a zoonotic infectious disease that can be transmitted from animals to humans. Commonly known as “undulant fever” …

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Malaria Antigen Test: Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Malaria Antigen Test

Introduction Malaria, caused by Plasmodium parasites, remains one of the most prevalent and deadly infectious diseases worldwide. Each year, millions of people are affected by this mosquito-borne illness, especially in tropical and subtropical regions. Early and accurate diagnosis of malaria is crucial for effective treatment …

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Aspergillus fumigatus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Aspergillus fumigatus colony morphology on Czapek-Dox agar plate

Introduction Aspergillus fumigatus is a filamentous fungus and one of the most common and clinically relevant species in the Aspergillus genus. It is widely distributed in the environment and can be found in soil, compost, decaying vegetation, and indoor environments. It is known for its …

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Aspergillus flavus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Aspergillus flavus colony morphology on Czapek Dox Agar plate

Introduction Aspergillus flavus is a filamentous fungus belonging to the Aspergillus genus, which comprises a wide variety of mold species. This particular species is commonly found in the environment and is known for its economic and medical significance. It is commonly associated with food and …

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Rhizopus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Sporangiophores, columellae and rhizoids of Rhizopus species in LPCB preparation of culture microscopy

Introduction Rhizopus is a genus of fungi belonging to the phylum Zygomycota and the class Mucoromycotina. It is one of the most well-known and common genera of the Mucoraceae family. Rhizopus species are saprophytic fungi, meaning they obtain nutrients by decomposing organic matter in their …

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Candida krusei: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Colony morphology of Candida krusei on SDA plate

Introduction Candida krusei is a species of yeast that belongs to the Candida genus. It is an opportunistic fungal pathogen known to cause infections in humans, particularly those with compromised immune systems or underlying medical conditions. C. krusei is an important member of the Candida …

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Mucor: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Mucor is a genus of fungi belonging to the family Mucoraceae within the phylum Zygomycota. These fungi are commonly known as mold and are ubiquitous in the environment, found in soil, decaying organic matter, and on various surfaces. Mucor species play essential roles in …

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Candida parapsilosis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Candida parapsilosis growth on Candida CHROMagar

Introduction Candida parapsilosis is a species of yeast that belongs to the Candida genus, which includes various fungi commonly found in the environment and on the human body. Candida species are part of the normal human microbiota, residing on the skin, mucous membranes, and gastrointestinal …

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Candida tropicalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Various Candida species grew on Candida CHROMAgar -Candida albicans (green), Candida tropicalis (cream-colored to light pink colonies), Candida glabrata (purple to lavender-color), Candida krusei(dark blue to mauve colonies), and Candida parapsilosis (cream-colored) as shown in the figure.

Introduction Candida tropicalis is a species of yeast, belonging to the Candida genus, commonly found in the environment and on the human body as part of the normal microbial flora. Similar to other Candida species, it can exist as a commensal organism, coexisting harmlessly with …

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Candida glabrata: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Yeast cells and hyphae of Candida glabrata in Giemsa stained smear of sputum microscopy at a magnification of 1000X

Introduction Candida glabrata is a species of yeast, specifically a type of fungus, that belongs to the Candida genus. It is commonly found in the environment and is also part of the normal microbial flora on the skin and mucous membranes of humans. While it …

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Neisseria meningitidis:Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Neisseria meningitidis, commonly known as meningococcus, is a Gram-negative bacterium that is a leading cause of meningitis and septicemia (blood poisoning) worldwide. Meningitis is an infection that affects the meninges, which are the protective membranes surrounding the brain and spinal cord. N.meningitidis can also …

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Neisseria gonorrhoeae:Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Neisseria gonorrhoeae, commonly known as gonococcus or simply gonorrhea, is a Gram-negative bacterium that causes the sexually transmitted infection (STI) known as gonorrhea. It is one of the most prevalent and widely recognized bacterial STIs worldwide, primarily transmitted through sexual contact with an infected …

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Haemophilus influenzae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Haemophilus influenzae is a Gram-negative bacterium that was originally believed to be the causative agent of influenza (the flu). However, it was later discovered that H. influenzae is not related to the viral infection that causes influenza. Instead, this bacterium is known for causing …

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Extended Spectrum Beta-Lactamase (ESBL): Introduction, List of Bacteria, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Extended spectrum beta lactamase (ESBL) detection using Double-Disc Synergy Test (DDST)

Introduction ESBL stands for Extended-Spectrum Beta-Lactamase. It refers to a group of enzymes produced by certain types of bacteria that can break down and inactivate a broad range of antibiotics called beta-lactams. Beta-lactams include commonly used antibiotics like penicillins, cephalosporins, and carbapenems. ESBL-producing bacteria are …

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Methicillin-resistant Staphylococcus aureus (MRSA): Introduction, Morphology, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Nasal Methicillin-resistant Staphylococcus aureus (MRSA) Carriage expressing Cefoxitin and Oxacillin Resistance on Muller-Hinton agar (MHA)

Introduction Methicillin-resistant Staphylococcus aureus, commonly known as MRSA, is a type of bacteria that has developed resistance to multiple antibiotics, including methicillin and other beta-lactam antibiotics like penicillin and cephalosporins. Staphylococcus aureus is a bacterium that can naturally colonize the skin and mucous membranes of …

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Listeria monocytogenes: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Listeria monocytogenes colony morphology on blood agar-Upper side

Introduction Listeria monocytogenes is a gram-positive, rod-shaped bacterium that is widely distributed in nature and can cause a serious foodborne infection known as listeriosis. It is one of the few bacterial species capable of growing at refrigeration temperatures, making it a significant concern for food …

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Immunochromatography: Introduction, Principle, Procedure, Result-Interpretation, Uses and Keynotes

Rapid tests based on Immunochromatography- Hepatitis A virus, Hepatitis E virus, Syphilis rapid test, ToRCH Rapid test and more

Introduction Immunochromatography, also known as lateral flow immunoassay or rapid test, is a widely used diagnostic technique that allows for the rapid and qualitative detection of specific target substances, such as antigens or antibodies, in various biological samples. It is a simple, cost-effective, and user-friendly …

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TORCH Panel: Introduction, Group of Test, Test Device, Clinical Significane, and Keynotes

TORCH Panel Test device showing Rubella IgM -Positive

Introduction and Group of Tests The TORCH panel is a group of blood tests used to screen for certain infections that can affect a developing fetus during pregnancy. The name “TORCH” is an acronym that stands for Toxoplasmosis, Other (syphilis, varicella-zoster virus, parvovirus B19), Rubella, …

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Dengue Fever Panel Test: Introduction, Test Procedure, Report, Test Method, Clinical Significance, Keynotes

Dengue Fever Panel Test: Introduction, Test Procedure, Report, Test Method, Clinical Significance, Keynotes

Introduction The Dengue Fever Panel test is a diagnostic test designed to detect and confirm the presence of the dengue virus or specific antibodies in a person’s blood. Dengue fever is a viral infection transmitted by mosquitoes, primarily the Aedes species, and is prevalent in …

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Salmonella Typhi IgM and IgG Test: Introduction, Test Report, Test Method, Clinical Significance, Keynotes

Salmonella rapid test

Introduction The Salmonella Typhi IgM and IgG test is a serological test used to detect antibodies produced by the immune system in response to Salmonella Typhi, the bacterium responsible for causing typhoid fever. This test helps in diagnosing and confirming cases of typhoid fever, a …

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Urine Routine Examination: Introduction, List of Test parameters,Test Report, Clinical Singificance,and Keynotes

Urine Routine Examination

Introduction Urine Routine Examination, also known as Urinalysis, is a common diagnostic test that provides valuable information about a person’s kidney function and overall health. It involves the analysis of a urine sample to assess various components and characteristics of the urine. This non-invasive test …

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Penicillium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Penicillium colony morphology on fungal culture medium

Introductio Penicillium is a genus of mold that belongs to the family Aspergillaceae within the fungal kingdom. It is one of the most important and well-known groups of fungi, particularly due to its significant impact on medicine, industry, and food production. The name “Penicillium” originates …

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Scedosporium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Scedosporium colony morphology on Sabouraud dextrose agar (SDA) of clinical specimen, pus culture

Introduction Scedosporium is a genus of fungi that belongs to the phylum Ascomycota. It is a medically important group of molds known for their ability to cause a wide range of infections in humans. The genus was first described in 1913 by Paul Vuillemin and …

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Acremonium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Acremonium colony characteristics on Sabouraud dextrose agar of clinical specimen, ear discharge

Introduction Acremonium is a genus of fungi that belongs to the family Hypocreaceae within the order Hypocreales. It is a diverse group of molds commonly found in soil, plant material, and decaying organic matter. They are ubiquitous in nature and can be found in various …

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Trichosporon: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Trichosporon colony morphology on potato dextrose agar (PDA) of clinical specimen culture

Introduction Trichosporon is a genus of yeast-like fungi that belongs to the phylum Basidiomycota. It is commonly found in the environment, especially in soil, water, and various organic matter. Trichosporon species can be opportunistic pathogens, and they are known to cause infections in both humans …

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Blood Culture: Introduction,Common Blood Pathogens, Test Report, Clinical Singificance,and Keynotes

BD BACTEC blood culture vial

Introduction Blood culture is a diagnostic test performed to detect the presence of microorganisms, such as bacteria and fungi, in the bloodstream. It is a critical tool used by healthcare providers to identify and treat bloodstream infections or septicemia, which can be life-threatening if not …

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Kidney Function Tests (KFTs): Introduction, Test Result, Unit, Reference Range, Test Methods,and Keynotes

Kidney Function Tests (KFTs)-Introduction, Test Result, Unit, Reference Range, Test Methods,and Keynotes

Introduction Kidney Function Tests (KFTs), also known as Renal Function Tests (RFTs), are a group of laboratory tests that provide important information about the functioning of the kidneys. These tests help doctors assess the health of the kidneys, detect kidney diseases, and monitor the effectiveness …

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24-hour Urine Protein Test: Introduction, ,Test Result, Unit, Reference Range, Clinical Significance,and Keynotes

24-hour Urine Protein Test Introduction, ,Test Result, Unit, Reference Range, Clinical Significance,and Keynotes

Introduction The 24-hour urine protein test is a diagnostic procedure used to measure the amount of protein excreted in a person’s urine over a 24-hour period. It is an essential test for assessing kidney function, identifying kidney diseases, and monitoring the progression of certain conditions. …

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Blood Iron Test: Introduction, Principle,Test Procedure,Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Blood Iron Test -Introduction, Principle,Test Procedure,Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Introduction The Blood Iron Test, also known as the Serum Iron Test, is a medical examination that measures the level of iron in your blood. Iron is an essential mineral that plays a crucial role in carrying oxygen throughout the body and is a key …

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E. cloacae: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

E. cloacae mucoid growth on CLED agar of Urine Culture

Introduction Enterobacter cloacae is a Gram-negative bacterium belonging to the Enterobacteriaceae family. It is a non-motile, facultative anaerobic, rod-shaped bacterium with a characteristic ability to ferment lactose. The name “cloacae” is derived from the Latin word “cloaca,” which means sewer or drain, indicating the bacterium’s …

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Helicobacter pylori Antigen Test: Introduction, Principle,Test Procedure,Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Helicobacter pylori Antigen Test- Introduction, Principle,Test Procedure,Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Introduction Introduction: Helicobacter pylori (H. pylori) is a bacterium that colonizes the human stomach and is a major cause of various gastrointestinal disorders, including gastritis, peptic ulcers, and even gastric cancer. The discovery of H. pylori‘s role in these diseases revolutionized the field of gastroenterology …

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Complete blood count (CBC): Introduction, Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Complete blood count (CBC) Introduction, Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Introduction A Complete Blood Count (CBC) is a common blood test that provides valuable information about the cellular components of the blood. It measures various parameters, such as red blood cells (RBCs), white blood cells (WBCs), and platelets, as well as other related indices. The …

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Renal Function Tests (RFTs): Introduction, Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Some most common renal function tests (RFTs)-Test Result, Unit, Reference Range,and Testing Methods

Introduction of Renal Function Tests (RFTs) Renal Function Tests (RFTs) are a group of laboratory tests that assess the functioning of the kidneys. These tests provide valuable information about the overall health and efficiency of the kidneys and help in diagnosing and monitoring various kidney …

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Urine Microalbumin Test: Introduction, Test Result, Unit, Reference Range, Testing Method,and Keynotes

Urine Microalbumin Test Introduction, Test Result, Unit, Reference Range, Testing Method,and Keynotes

Introduction The Urine Microalbumin Test is a diagnostic test that measures the levels of microalbumin in urine. It is primarily used to assess kidney function and detect early signs of kidney damage, particularly in individuals with conditions such as diabetes, hypertension, or other conditions that …

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Cryptococcus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Cryptococcus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Cryptococcus is a genus of fungi that includes several species, the most common of which is Cryptococcus neoformans. These fungi are widely distributed in the environment, particularly in soil and bird droppings. Cryptococcus species are known for causing opportunistic infections, primarily affecting individuals with …

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Chemiluminescent Immunoassay (CLIA) Analyzer: Introduction, Principle, Test Requirements, Handling Procedure, Uses, and Keynotes

Chemiluminescent Immunoassay (CLIA) Analyzer: Introduction, Principle, Test Requirements, Handling Procedure, Uses, and Keynotes

Introduction A chemiluminescent immunoassay (CLIA) analyzer is a diagnostic instrument used in clinical laboratories to perform immunoassays based on chemiluminescence detection. CLIA is a technique that utilizes the emission of light resulting from a chemical reaction to detect and quantify specific analytes present in a …

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Vitrous Dry Chemistry Analyzer: Introduction, Principle, Test Requirements, Handling Procedure, Uses, and Keynotes

Vitrous Dry Chemistry Analyzer: Introduction, Principle, Test Requirements, Handling Procedure, Uses, and Keynotes

Introduction of Vitrous Dry Chemistry Analyzer The Vitros Dry Chemistry Analyzer is a diagnostic instrument used in clinical laboratories for performing a variety of biochemical tests. It is manufactured by Ortho Clinical Diagnostics, a company specializing in clinical laboratory equipment and reagents. The Vitros Analyzer …

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TB PCR for Sputum: Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance, and Keynotes

TB PCR for Sputum-Mycobacterium tuberculosis Detected but Rifampicin Resistance not Detection

Introduction TB PCR (polymerase chain reaction) for sputum is a laboratory test used in the diagnosis of tuberculosis (TB). It is a molecular diagnostic technique that detects the presence of Mycobacterium tuberculosis (the bacterium that causes TB) DNA in sputum samples. Traditional methods of diagnosing …

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SARS-CoV-2 Total Antibody: Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance,and Keynotes

SARS-CoV-2 Total Antibody Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance,and Keynotes

Introduction SARS-CoV-2, also known as the novel coronavirus, is the virus responsible for the COVID-19 pandemic. The SARS-CoV-2 Total Antibody assay is a laboratory test designed to detect and measure the presence of antibodies against SARS-CoV-2 in a person’s blood sample. When a person is …

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Anti -TPO Assay: Introduction, Test Result, Unit, Normal Range, Assaying Method, and Keynotes

Anti -TPO Assay Introduction, Test Result, Unit, Normal Range, Assaying Method, and Keynotes

Introduction The Anti-Thyroid Peroxidase (Anti-TPO) assay is a laboratory test used to detect and measure the presence of antibodies against thyroid peroxidase in the blood. Thyroid peroxidase is an enzyme involved in the production of thyroid hormones in the thyroid gland. The Anti-TPO assay is …

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Candida albicans: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Creamy-white, smooth colonies of Candida albicans on Sabouraud dextrose agar (SDA)

Introduction Candida albicans is a species of yeast that is part of the normal microbial flora found in the human body. It is the most common fungal pathogen in humans and can cause a wide range of infections, especially in individuals with compromised immune systems. …

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Bacillus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Bacillus Endospores Microscopy

Introduction Bacillus is a genus of gram-positive, rod-shaped bacteria that belongs to the phylum Firmicutes. These bacteria are commonly found in various environments, including soil, water, and the gastrointestinal tracts of animals. The name “Bacillus” is derived from the Latin word for “rod.” Bacillus species …

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Tacrolimus: Introduction, Normal Range, Test Method, Clinical Significance, and Keynotes

Tacrolimus Introduction, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction Tacrolimus, also known by its trade name Prograf, is an immunosuppressive medication commonly used in organ transplantation and the treatment of certain autoimmune disorders. It belongs to a class of drugs called calcineurin inhibitors. It works by inhibiting the activity of calcineurin, a protein …

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Pseudomonas aeruginosa: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Pseudomonas aeruginosa non-lactose fermenting (NLF) colony and mucoid lactose fermenting colony of Klebsiella pneumoniae on MacConkey medium

Introduction Pseudomonas aeruginosa is a Gram-negative bacterium that is widely distributed in nature and is known for its versatility and adaptability. It is an opportunistic pathogen capable of causing infections in humans, particularly in individuals with weakened immune systems or underlying medical conditions. P. aeruginosa …

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Candida: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Cream-coloredpasty and glistening colonies of Candida albicans on Sabouraud-Dextroseagar (SDA) plate

Introduction Candida is a genus of yeast that is commonly found in the environment and is also a part of the normal microbial flora in the human body. The most well-known species within this genus is Candida albicans, although there are over 20 different species …

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Quorum Sensing: Introduction, Its Role in Virulence, and Possibilities for Its Control, and Keynotes

Another organism responsible for quorum sensing- Staphylococcus aureus and its growth on blood agar of clinical specimen, pus

Introduction Quorum sensing (QS) is a sophisticated cell-to-cell communication system used by bacteria to coordinate group behaviors based on the population density. It enables bacterial cells to communicate and synchronize their activities by detecting and responding to small signaling molecules called autoinducers. In quorum sensing, …

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Acinetobacter: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Acinetobacter: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Acinetobacter is a genus of Gram-negative bacteria that are commonly found in the environment, including soil, water, and healthcare settings. While some species of Acinetobacter are harmless and part of the normal flora on human skin, others can cause opportunistic infections, particularly in healthcare-associated …

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First Aid Box: Introduction, Basic items, Handling Procedure, Application, Precaution, and Keynotes

First Aid Box: Introduction, Basic items, Handling Procedure, Application, Precaution, and Keynotes

Introduction A first aid box, also known as a first aid kit or medical kit, is a collection of medical supplies and equipment that is used to provide initial medical assistance and care in the event of an injury, illness, or emergency. It is an …

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Spill Kit: Introduction, Kit Content, Handling Procedure, Application, Precaution, and Keynotes

Spill Kit: Introduction, Kit Content, Handling Procedure, Application, Precaution, and Keynotes

Introduction A spill kit is a collection of tools, materials, and equipment designed to quickly and effectively respond to spills of hazardous substances. Spill kits are essential in various settings, including laboratories, industrial facilities, healthcare facilities, and transportation sectors. They help mitigate the risks associated …

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Biofilm: Introduction, Composition, Application, and Keynotes

Biofilm- Introduction, Composition, Application, and Keynotes

Introduction Biofilm refers to a complex community of microorganisms, such as bacteria, fungi, or algae, that adhere to surfaces and are encased within a self-produced matrix of extracellular polymeric substances (EPS). These microorganisms form a structured, organized, and protective community within the biofilm. Key points …

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Dermatophyte: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Dermatophyte: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Dermatophytes, also known as dermatophyte fungi or simply “ringworm fungi,” are a group of fungi that cause infections in the skin, hair, and nails of humans and animals. These fungi primarily belong to three genera: Trichophyton, Microsporum, and Epidermophyton. Dermatophytes are specialized organisms that …

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Pus cells in Urine lacking growth on Culture Media: Introduction, Possible Reasons, and Keynotes

Pus cells in Urine lacking growth on Culture Media

Introduction of Pus cells in Urine lacking growth on Culture Media The presence of pus cells (also known as leukocytes or white blood cells) in urine without the growth of microorganisms on culture media can indicate a condition called sterile pyuria. Sterile pyuria refers to …

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Drug of Abuse (DOA) Panel: Introduction, Test Panel, Report, Application, and Keynotes

Drug of Abuse (DOA) Panel Test Report

Introduction of Drug of Abuse (DOA) Panel Drug of Abuse (DOA) panels are laboratory tests that analyze urine samples to detect the presence of drugs or their metabolites. These panels are used to screen for the use of illicit drugs and prescription medications that have …

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Strongyloides: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Strongyloides- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Strongyloides is a genus of parasitic nematodes (roundworms) that includes the species Strongyloides stercoralis, commonly known as the human threadworm or threadworm. These tiny worms are parasitic in nature and can infect humans and other animals. Here is an introduction to Strongyloides: Morphology Strongyloides …

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Absidia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Absidia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Absidia is a genus of filamentous fungi belonging to the class Zygomycetes. It is a member of the family Mucoraceae, which includes several other genera of molds. The genus Absidia comprises around 40 recognized species, with Absidia corymbifera being the most well-known and frequently …

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Urinary Tract Infection (UTI): Introduction, Types, Sign and Symptoms, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Urinary Tract Infection (UTI) due to E. coli

Introduction A urinary tract infection (UTI) is a bacterial infection that affects any part of the urinary system, which includes the kidneys, bladder, ureters, and urethra. UTIs are among the most common types of infections, and they can occur in people of all ages, although …

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Sterile Bacteriuria: Introduction, Causes, Related Report, and Keynotes

Urine routine examination report-Sterile Bacteriuria

Introduction Sterile bacteriuria refers to the presence of bacteria in the urine without any signs or symptoms of a urinary tract infection (UTI). In a healthy individual, the urinary tract, including the bladder and kidneys, is generally considered to be sterile. However, in some cases, …

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Bipolar Staining: Introduction, Uses, and Keynotes

Bipolar Staining: Introduction, Uses, and Keynotes

Introduction Bipolar staining refers to a specific pattern of staining observed in certain bacteria during Gram staining, a common laboratory technique used to classify bacteria based on their cell wall characteristics. In a typical Gram stain, bacterial cells are first stained with crystal violet, followed …

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Sterile Pyuria: Introduction, Causes, Related Report, and Keynotes

Urine Culture -No growth on Culture Media

Introduction Sterile pyuria refers to the presence of white blood cells (leukocytes) in the urine without the presence of bacteria or other microorganisms that typically cause urinary tract infections (UTIs). It is called “sterile” because no significant bacterial growth is observed in the urine culture …

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Burkholderia cepacia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Burkholderia cepacia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Burkholderia cepacia is a species of gram-negative bacteria within the Burkholderia genus. It is a versatile and opportunistic pathogen that can cause infections in both immunocompromised and healthy individuals. B. cepacia is a significant concern, particularly in individuals with cystic fibrosis (CF) and other …

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Enterococcus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Enterococcus faecium colony characteristics on CLED agar of Urine Culture

Introduction Enterococcus is a genus of Gram-positive bacteria that are part of the normal microbial flora of humans and animals. They are facultative anaerobes, meaning they can survive in both the presence and absence of oxygen. Enterococcus species are known for their ability to thrive …

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CA19-9 Test: Introduction, Test Method, Normal Range, Clinical Significance, and Keynotes

CA19-9 Test Result, Normal Rangle, and Clinical Significance

Introduction CA19-9, also known as carbohydrate antigen 19-9, is a tumor marker commonly used in clinical practice. It is a type of glycoprotein, specifically a mucin-like antigen, that is produced by various types of cells, including epithelial cells lining the gastrointestinal tract, gallbladder, and pancreatic …

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Antimicrobial Susceptibility Testing (AST): Introduction, Principle, Test Methods, Test Requirements, Procedure, and Result Interpretation, Application, and Keynotes

Antimicrobial Susceptibility Testing (AST): Introduction, Principle, Test Methods, Test Requirements, Procedure, and Result Interpretation, Application, and Keynotes

Introduction Antimicrobial Susceptibility Testing (AST) is a laboratory technique used to assess the effectiveness of antimicrobial agents, such as antibiotics, against specific microorganisms. The aim of AST is to determine the susceptibility or resistance of a microorganism to different antimicrobial drugs. This information is crucial …

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Tryptone Soy Broth: Introduction, Composition, Principle, Preparation, Testing Procedure, Colony Characteristics, Limitations, and Keynotes

BD Bactec Tryptone Soy Broth bottles freshly inoculated blood specimens for culture

Introduction and Composition Tryptone Soy Broth (TSB) is a versatile and widely used liquid medium in microbiology. It is a nutrient-rich broth that supports the growth of a wide range of bacteria, making it suitable for general cultivation, enumeration, and maintenance of bacterial cultures. TSB …

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Shigella sonnei: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Shigella sonnei in wet mount of Tryptone soy broth microscopy

Introduction Shigella sonnei is a bacterium that is responsible for causing shigellosis, a type of bacterial infection affecting the digestive system. Shigellosis is commonly characterized by symptoms such as diarrhea, abdominal pain, fever, and occasionally, bloody stools. It is a specific serotype of the Shigella …

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Streptococcus oralis:Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Streptococcus oralis colony morphology on MacConkey agar

Introduction Streptococcus oralis is a gram-positive bacterium that belongs to the genus Streptococcus. It is a commensal bacterium that commonly inhabits the human oral cavity, along with other oral streptococci. S. oralis is considered a part of the normal oral microbiota and can be found …

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Tacrolimus Test: Introduction,Normal Range, Clinical Significance, and Keynotes

Tacrolimus Test-Normal Range, Test method, and Clinical Significance

Introduction A Tacrolimus test is a medical test that measures the level of Tacrolimus in the blood. Tacrolimus is a medication that is commonly used in organ transplantation to prevent rejection of the transplanted organ. It belongs to a class of drugs known as immunosuppressants. …

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Candida Chromagar: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Candida Chromagar: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Introduction Candida Chromagar is a selective and differential agar medium used for the isolation and identification of Candida species. HiMedia is a well-known manufacturer of microbiological culture media and laboratory products. Here are some keywords related to this agar: Principle The principle of Candida Chromagar …

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SARS-COV-2 Antigen Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpreation, Keynotes

SARS-COV-2 Antigen Test Strong Positive-Upper while weak positive -lower test device

Introduction SARS-CoV-2 antigen tests, also known as rapid antigen tests or rapid diagnostic tests (RDTs), are diagnostic tests designed to detect specific proteins or antigens from the SARS-CoV-2 virus, which is responsible for the COVID-19 disease. These tests are used to quickly identify active COVID-19 …

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X-Match: Introduction, Principle, Test-Procedure, Result-Interpretation, Application, and Keynotes

X-Match: Introduction, Principle, Test-Procedure, Result-Interpretation, Application, and Keynotes

Introduction of X-Match The crossmatch is an important step in ensuring compatibility between the donor’s blood and the recipient’s blood before a blood transfusion takes place. It is performed to detect any potential incompatibilities that could lead to adverse reactions in the recipient. During a …

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E (Epsilometer) Test-Introduction, Principle, Purpose, Procedure, Result-Interpretation, Advantages, and Disadvantages

Polymyxin B and Colistin E- Test for Acinetobacter

Introduction The E-test, also known as the Epsilometer test, is a laboratory technique used to determine the antimicrobial susceptibility of microorganisms, particularly bacteria. It is a quantitative method that provides information about the minimum inhibitory concentration (MIC) of an antimicrobial agent against a specific organism. …

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Serum Protein Electrophoresis: Introduction, Pattern, Application, and Keynotes

Serum Protein Electrophoresis

Introduction Serum protein electrophoresis (SPEP) is a laboratory test used to separate and analyze the proteins present in a person’s blood serum. It is a common diagnostic tool in clinical medicine and helps in the evaluation and diagnosis of various conditions, particularly those affecting the …

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Citrobacter freundii: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Citrobacter freundii growth on CLED agar of Urine Culture

Introduction Citrobacter freundii is a Gram-negative bacterium belonging to the Enterobacteriaceae family. It is commonly found in the environment, including soil, water, and the intestinal tracts of humans and animals. Citrobacter freundii is an opportunistic pathogen, meaning it can cause infections in individuals with weakened …

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Morganella: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Morganella morganii Colony Morphology on CLED agar of Urine Culture

Introduction Morganella is a genus of bacteria belonging to the family Enterobacteriaceae. The genus was named after the bacteriologist John Morgan. Morganella species are gram-negative, rod-shaped bacteria that are commonly found in the environment, including soil, water, and sewage. One species of particular interest is …

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Serratia ficaria: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment,Prevention, and Keynotes

Serratia ficaria on CLED Agar of Urine Culture

Introduction Serratia ficaria is a bacterial species that belongs to the genus Serratia. It is a Gram-negative, rod-shaped bacterium that is commonly found in various environmental niches, including soil, water, and plants. Serratia ficaria is known for its ability to produce a distinctive red pigment …

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Staphylococcus lentus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Serratia ficaria on CLED Agar of Urine Culture

Introduction Staphylococcus lentus is a species of Gram-positive bacteria belonging to the genus Staphylococcus. It is classified as a coagulase-negative staphylococcus (CoNS), which means it does not produce the enzyme coagulase. CoNS are commonly found as part of the normal human skin and mucous membrane …

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Alcohol: Definition, Types, Effects, History, and Keynotes

Definition Alcohol is a chemical compound that is widely consumed in the form of alcoholic beverages. Chemically, alcohol refers to a class of organic compounds known as alcohols, with the most common type being ethanol (also called ethyl alcohol). Ethanol is produced through the fermentation …

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Smoking: Definition, Types, Effects, History, and Keynotes

Definition Smoking refers to the act of inhaling and exhaling the smoke produced by burning tobacco or other substances, typically through the use of cigarettes, cigars, or pipes. It is a voluntary behavior in which the smoke is drawn into the mouth and lungs, allowing …

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Cancer: Introduction, Types, Sign and Symptoms, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

Introduction Cancer is a complex and diverse group of diseases characterized by the uncontrolled growth and spread of abnormal cells in the body. It can affect virtually any organ or tissue, and if left untreated, it can be life-threatening. Normal cells in the body grow, …

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Blood Pressure (BP): Introduction, Types, Sign and Symptoms, Diagnosis, Treatment, and Keynotes

Introduction Blood pressure (BP) is the force exerted by circulating blood against the walls of the blood vessels. It is an essential physiological parameter that reflects the pressure within the arterial system as the heart pumps blood throughout the body. BP is expressed as two …

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Diabetes: Introduction,Types,Sign and Symptoms, Lab Diagnosis, Treatment, and Keynotes

Sucking Blood in Glucometer for Blood Sugar Monitoring

Introduction Diabetes mellitus (DM), commonly referred to as diabetes, is a chronic metabolic disorder characterized by high blood sugar levels over a prolonged period. It occurs when the body either does not produce enough insulin or fails to use insulin effectively. Insulin is a hormone …

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abGenix Nucleic Acid Extractor: Introduction, Principle, Test Requirements, Test Procedure, Uses, and Keynotes

abGenix Nucleic Acid Extractor: Introduction, Principle, Test Requirements, Test Procedure, Uses, and Keynotes

Introduction Nucleic acid extraction is a fundamental step in molecular biology and genetics research, as well as in various diagnostic applications. It involves isolating and purifying DNA or RNA from biological samples, such as cells, tissues, blood, or other body fluids. This process allows for …

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Serratia fonticola: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment,Prevention, and Keynotes

Gram negative bacilli or rods of Serratia fonticola in Bronchoalveolar lavage (BAL) sample Gram staining

Introduction Serratia fonticola is a Gram-negative bacterium that belongs to the genus Serratia. It was first isolated and described in 1998 from water samples in a freshwater spring in California, USA. Since its discovery, S. fonticola has been identified in various environmental sources, including soil, …

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Medical Lab Assistant: Introduction, Scope, Keynotes, and Curriculum PDF

Medical Lab Assistant-Scope in Clinical Laboratory

Introduction A medical lab assistant plays a crucial role in the healthcare industry. They work in medical laboratories, clinics, hospitals, research facilities, and other healthcare settings, performing various laboratory tests and procedures to assist in the diagnosis, treatment, and prevention of diseases. Here are some …

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Formal Ether Concentration Technique-Introduction, Principle, Test Requirements, Test Procedure, Uses, and Keynotes

Formal Ether Concentration Technique

Introduction The Formal Ether Concentration Technique is a laboratory method used to concentrate parasitic organisms, such as eggs, larvae, or cysts, from a fecal sample. It is particularly useful for the detection of intestinal parasites that may be present in low numbers and are difficult …

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Certificate in Medical Laboratory Technician (CMLT): Introduction, Scope, Keynotes, and Curriculum PDF

Certificate in Medical Laboratory Technician (CMLT)-Introduction, Scope, Keynotes, and Curriculum PDF

Introduction Certificate in Medical Laboratory Technician (CMLT) is a diploma-level course in the field of medical laboratory technology. It is a one-year program whereas in Nepal 3 years program after matriculation (SLC/SEE) that trains students to perform laboratory tests and procedures that aid in the …

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Bioer Automatic Nucleic Acid Purification Analyzer: Introduction, Principle, Test Requirements, Handling Procedure, Uses, and Keynotes

Bioer Automatic Nucleic Acid Purification Analyzer- Introduction, Principle, Test Requirements, Handling Procedure, Uses, and Keynotes

Introduction Bioer Automatic Nucleic Acid Purification Analyzer is an automated system designed to isolate and purify nucleic acid from various samples. It is manufactured by Bioer Technology Co., Ltd, a company based in China. The system utilizes magnetic bead technology and features a compact design …

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Automatic Tissue Processor: Introduction, Principle, Types, Handling Procedure, Uses, and Keynotes

Automatic Tissue Processor

Introduction An automatic tissue processor is a laboratory instrument . It automates the process of tissue fixation, dehydration, clearing, and embedding for histological analysis. This instrument is widely used in pathology laboratories for preparing tissue samples for examination under a microscope. The automatic tissue processor …

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Laura Smart Urine Analyzer-Introduction, Parts, Types, Application, and Keynotes

Erba Transia Laura Smart Urine Analyzer (Semi-Automatic Analyzer)

Introduction The Laura Smart Urine Analyzer is a medical device used for the automatic analysis of urine samples. This analyzer employs advanced optical technology to provide fast and accurate results for a variety of urine parameters, including glucose, protein, blood, leukocytes, nitrites, urobilinogen, ketones, bilirubin, …

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Sperm Quality Analyzer-Introduction, Parts, Types, Application, and Keynotes

Sperm Quality Analyzer-SQAIIC-P

Introduction Sperm Quality Analyzer (SQA) is a medical device. It is designed to measure the concentration, motility, and morphology of sperm in semen samples. The device uses optical technology to provide rapid and accurate results that can be used for diagnosis and monitoring of male …

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Aeromonas sobria: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Aeromonas sobria colony morphology on CLED agar of Urine Culture after 4 days of incubation

Introduction Aeromonas sobria is a species of Gram-negative bacteria that belongs to the Aeromonas genus. It is a rod-shaped, facultative anaerobic bacterium that is commonly found in aquatic environments, such as freshwater and brackish water. A. sobria can cause a range of infections in humans, …

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4 Plus Channel qPCR Platforms: Introduction, Uses, and Keynotes

QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop

Introduction 4+ channel qPCR platforms refer to qPCR instruments that are capable of simultaneously detecting and quantifying multiple fluorescent signals from four or more different targets within a single reaction tube. These instruments are often used in gene expression studies, genotyping, and other applications where …

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Nanometer: Introduction, Principle, Handling Procedure, Application, and Keynotes

Nanometer

Introduction A nanometer (nm) is a unit of length in the metric system that is equal to one billionth of a meter. It is commonly used in the field of nanotechnology to describe the size of particles and structures that are too small to be …

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Enterococcus durans: Introduction, Classification, Morphology, Pathogenicity, Lab Diagnosis, Treatment, and Prevention

Enterococcus durans Colony Morphology on CLED Agar of Urine Culture

Introduction Enterococcus durans is a Gram-positive, facultative anaerobic bacterium that belongs to the family Enterococcaceae. It is commonly found in the gastrointestinal tract of humans and animals, as well as in soil and water. It is known for its ability to survive in harsh environments, …

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Yersinia: Introduction, Classification Morphology, Pathogenicity, Lab Diagnosis, Treatment, and Prevention

Yersinia enterocolitica colony morphology on CLED agar of Urine Culture

Introduction Yersinia is a genus of bacteria that includes several species, some of which are pathogenic to humans. The most well-known species within this genus are Yersinia pestis, the causative agent of the bubonic and pneumonic plague, and Y. enterocolitica, a cause of gastrointestinal infections. …

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Giardia- Introduction, Morphology, Life- Cycle, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Cysts of Giardia lamblia in iodine wet mount of feces microscopic examination

Introduction of Giardia Giardia is a genus of parasitic protozoa that can infect the small intestine of mammals, including humans. The species that infects humans is called Giardia lamblia or Giardia intestinalis. It is one of the most common causes of waterborne disease in the …

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Enterobius: Introduction, Classification Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Egg of Enterobius vermicularis in urine microscopy of a child-

Introduction of Enterobius Enterobius is a genus of parasitic worms, commonly known as pinworms, which infect the human gastrointestinal tract. The most common species of Enterobius that infects humans is Enterobius vermicularis, also known as the human pinworm. These worms are usually found in the …

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Raoultella: Introduction, Classification Morphology, Pathogenicity, Lab Diagnosis, Treatment, and Prevention

Raoultella ornithinolytica Colony Morphology on CLED agar of Urine Culture

Introduction Raoultella is a genus of gram-negative, non-motile, aerobic bacteria belonging to the Enterobacteriaceae family. The genus was named after Dr. Didier Raoult, a French microbiologist who made significant contributions to the study of Rickettsia. It was first described in 2001 as a distinct genus …

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Pantoea: Introduction, Classification Morphology, Pathogenicity, Lab Diagnosis, Treatment, and Prevention

Pantoea in Gram Staining of Culture Microscopy at Magnification of 4000X

Introduction Pantoea is a genus of gram-negative bacteria that belong to the family Enterobacteriaceae. These bacteria are commonly found in soil, water, and plant surfaces, and some species are known to be associated with plants, animals, and humans as opportunistic pathogens. There are several species …

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Women’s Health Package (below 40):Introduction, Related Assays, and Keynotes

Introduction of Women’s Health Package (below 40) The Women’s Health Package for women under 40 is a comprehensive set of healthcare services designed to address the specific health needs and concerns of younger women. This package is tailored to women in their reproductive years and …

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Women’s Health Package: Introduction, Related Assays, and Keynotes

Introduction A women’s health package is a comprehensive medical assessment program that is designed to evaluate the overall health and wellness of women. This package typically includes a range of tests and screenings that are tailored to the unique health needs of women, such as …

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Mycology Reference Laboratory: Introduction, Requirements, Mycological Tests, Importance and Keynotes

Introduction of Mycology Reference Laboratory A Mycology Reference Laboratory is a specialized laboratory that focuses on the identification, characterization, and study of fungi. Fungi are a diverse group of organisms that can cause a variety of infections in humans, animals, and plants, as well as …

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Child Health Checkup Package: Introduction, Related assays, and Keynotes

Introduction A child health checkup package is a medical assessment program designed to evaluate the overall health and development of children. This package typically includes a range of tests and screenings that are tailored to the unique health needs of children, such as growth and …

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Biomedical Waste: Introduction, Types, Segregation, and Management

Introduction of Biomedical Waste Biomedical waste refers to any waste that is generated during the diagnosis, treatment, or immunization of human beings or animals, or in research activities involving biological materials. This includes waste that may contain infectious agents, such as bacteria and viruses, as …

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Post-Covid Package: Introduction, Related Assays, and Keynotes

Introduction The post-Covid package is a medical assessment program designed for individuals who have recovered from Covid-19. This package typically includes a range of tests and screenings that are tailored to the unique health needs of Covid-19 survivors, who may experience lingering symptoms and complications …

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Lung Checkup Package: Introduction, Related Laboratory Tests, and Keynotes

Introduction A lung checkup package is a medical assessment program that is specifically designed to evaluate the health of an individual’s lungs. This package typically includes a series of diagnostic tests and screenings that are performed by medical professionals to assess the overall function and …

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Senior Health Lab Test Package (Male): Introduction, Related assays, and Keynotes

Introduction of Senior Health Lab Test Package (Male) There is no single lab test package that is specifically designed for senior men. However, there are certain lab tests that are recommended for older adults, especially men, as they are more susceptible to certain health conditions. …

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Cardiac Screening Package: Introduction, Related Laboratory Tests, and Keynotes

Introduction of Cardiac Screening Package A cardiac screening package is a set of tests that are used to assess the health of the heart and cardiovascular system. These tests are typically recommended for individuals who are at risk for developing heart disease or who have …

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General Health Checkup Package: Introduction, Related assays, and Keynotes

Introduction of General Health Checkup Package A general health checkup package typically includes a combination of tests that evaluate overall health status. The following are some of the common tests included in this package: The results of these tests can provide valuable information about overall …

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Thyroid Lab Test Screen Package: Introduction, Related Assays, and Keynotes

Introduction of Thyroid Lab Test Screen Package The thyroid lab test screen package typically includes a combination of tests that evaluate thyroid function. The following are some of the common tests included in this package: The results of these tests can provide valuable information about …

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Senior Health Lab Test Package(Female): Introduction, Related assays, and Keynotes

Introduction of Senior Health Lab Test Package There is no single lab test package that is specifically designed for senior women. However, there are certain lab tests that are recommended for older adults, especially women, as they are more susceptible to certain health conditions. Here …

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Pneumocystis: Introduction, Classification, Morphology, Pathogenicity Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction of Pneumocystis It is thought to be a protozoan, usually found in animals and occasionally in men, especially in newborns and young children. It causes typical pneumonia ( interstitial plasma cellular pneumonia) which is often fatal. The parasites appear as small round ‘ cysts’ …

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Hypertension Lab Test Package: Introduction, Related Laboratory Tests, and Keynotes

Introduction of Hypertension Lab Test Package There is no single lab test package that is used to diagnose hypertension, also known as high blood pressure. Hypertension is typically diagnosed through a combination of medical history, physical exam, and blood pressure measurements taken at different times. …

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Complete Diabetes Package: Introduction, Related Assays, and Keynotes

Introduction of Complete Diabetes Package A Complete Diabetes Package typically includes a series of tests that help diagnose and monitor diabetes and its related complications. Here are some common tests included in a Complete Diabetes Package: It’s important to consult with your healthcare provider to …

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Genetic Lab Test Package: Introduction, Test Available, and Keynotes

Introduction of Genetic Lab Test Package A Genetic Lab Test Package is a healthcare program that focuses on analyzing a patient’s DNA to identify potential genetic risks for certain diseases or conditions. The package includes a series of genetic tests that can help identify specific …

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Cardiac Checkup Package: Introduction, Related Laboratory Tests, and Keynotes

Introduction of Cardiac Checkup Package A Cardiac Checkup Package is a healthcare program designed to assess an individual’s cardiovascular health and detect any potential risks or issues related to the heart and blood vessels. The package typically includes a series of tests and evaluations that …

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Bone and Joint Care Package: Introduction Panel of Tests, and Keynotes

Introduction of Bone and Joint Care Lab Test Package A Bone and Joint Care Lab Test Package is a healthcare program that focuses on diagnosing and monitoring bone and joint-related problems through laboratory tests. This package is designed to provide comprehensive lab testing services to …

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COVID-19 Post-Recovery Package: Introduction, Related Assays, and Keynotes

Introduction of COVID-19 Post-Recovery Package COVID-19 post-recovery package may include a range of services and support to help individuals recover from the physical, emotional, and financial effects of the illness. The specific package may vary depending on the severity of the illness and the individual’s …

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Diabetes Screening Lab Test Package: Introduction, Related Assays, and Keynotes

Introduction of Diabetes Screening Lab Test Package There are several laboratory tests used for diabetes screening, but some common tests that are included in diabetes screening lab test package are: It is important to note that diabetes screening tests may vary depending on the age, …

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Haematology Test: Introduction, Group of Tests, Description, and Keynotes

Introduction of Haematology Test Hematology tests are a group of laboratory tests that evaluate blood and blood-forming tissues. They are commonly used to diagnose and monitor conditions related to the blood, such as anemia, leukemia, and clotting disorders. These tests are typically performed on a …

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Healthy Child Lab Test Package: Introduction, Related Assays, and Keynotes

Introduction of Healthy Child Lab Test Package The “Healthy Child Lab Test Package” is a bundle of laboratory tests that are designed to help assess the overall health of a child. These tests may include: The specific tests included in this package may vary depending …

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Cancer Screening Panel Tests (Female): Introduction, Related assays, and Keynotes

Cancer Screening Panel Tests-Female Cancer screening panel tests are a group of medical tests that are designed to detect different types of cancer in the body. These tests can be used to screen for cancer in individuals who do not have any symptoms or to …

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Post-Covid Test Package: Introduction, Package Details, Benefits, and Conclusion

Introduction of Post-Covid Test Package The COVID-19 pandemic has affected the world in unprecedented ways, and testing for the virus has become a crucial tool in controlling its spread. With the development of vaccines, testing is still important for the early detection and containment of …

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Fluorometer: Introduction, Principle, Test Requirements, Handling, Result-Interpretation, and Keynotes

Flurometer

Introduction of Fluorometer A fluorometer is an analytical instrument used to measure the fluorescence emitted by a sample. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength. Fluorometers are widely used in fields …

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RFT and LFT Tests: Introduction, Test Result, Unit, Normal Range, Test Methods, and Keynotes

Introduction of RFT and LFT Tests Renal function tests (RFT) are a set of medical tests that evaluate how well the kidneys are functioning. The kidneys are essential organs that filter waste and excess fluid from the blood, and they also help to regulate blood …

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Gel Documentation System: Introduction, Principle, Components, and Uses

Capturing the image of the gel using the gel documentation system

Introduction A Gel Documentation System (GDS) is a laboratory instrument used to visualize and document DNA, RNA, and protein samples that have been separated by gel electrophoresis. Gel electrophoresis is a common technique used in molecular biology for separating and analyzing biological molecules based on …

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Coagulation Test: Introduction, List of Tests, Description, and Keynotes

Blood Clotting Time Determination by Using Capillary Method

Introduction of Coagulation Test A coagulation test is a group of clinical laboratory tests pertaining to the coagulation profile that is performed to evaluate the blood’s ability to form clots. These tests are important for the diagnosis and management of bleeding disorders, as well as …

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Immunoassay: Introduction, Subgroups, List of Tests, and Keynotes

HIV ELISA Test Kit

Introduction of Immunoassay Immunoassay is a laboratory technique that uses antibodies to detect the presence and quantity of a substance in a sample. It relies on the specific binding between an antigen (the substance being detected) and an antibody (a protein produced by the immune …

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Updated Clinical Laboratory Reporting System: Introduction, Discussion, and Keynotes

Routine Urine Examination-Pus cells Reporting Reference

Introduction of Updated Clinical Laboratory Reporting System An updated clinical laboratory reporting system is a system that provides more comprehensive and standardized reporting of laboratory test results. The purpose of this system is to improve the clarity and usefulness of laboratory reports for healthcare providers, …

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Medical Lab Tests: Introduction, Panel of Tests, and Keynotes

Introduction of Medical Lab Tests Medical lab tests are tests performed on samples of blood, urine, or other body fluids or tissues to help diagnose, treat, and monitor various medical conditions. These tests provide important information about the functioning of organs and body systems, identify …

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Viral Hepatitis- Introduction,Test Results, Test Methods, and Keynotes

Viral Hepatitis-Test Results, Test Methods, and Keynotes

Introduction Viral hepatitis refers to a group of infectious diseases caused by different types of hepatitis viruses, including hepatitis A, B, C, D, and E. These viruses can cause inflammation and damage to the liver, leading to a range of symptoms from mild to severe. …

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Liver Function Test (LFT): Introduction, Test Result, Unit, Reference Range, Testing Methods,and Keynotes

Liver Function Test (LFT)-Test Result, Unit, Reference Range, and Testing Methods

Introduction of Liver Function Test (LFT) Liver Function Test (LFT) is a blood test that is used to evaluate the health of the liver and to detect any liver damage or disease. The liver is a vital organ in the body that plays a crucial …

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Escherichia coli: Introduction, Pathogenic Strains and Preventive Measures

Escherichia coli in Gram Staining of Culture Microscopy at a Magnification of 4000X

Introduction of Escherichia coli Escherichia coli (E. coli) is a type of bacteria that is commonly found in the intestines of humans and animals. While most strains of E. coli are harmless, some can cause illness, such as urinary tract infections, gastrointestinal infections, and even …

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Motility of Bacteria: Introduction, List of Motile and Non-motile Bacteria, Keynotes, and Related Videos

Introduction For Motility of Bacteria Bacteria are motile due to having flagella (singular -flagellum). Some common motile bacteria are E.coli, Salmonella, Proteus, Pseudomonas, Citrobacter, and Enterobacter while non-motile are Klebsiella, Shigella, and Acinetobacter. Motility Types of Different Microbes S.No Motility Types Microbes 1. Darting Motility …

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Helicobacter pylori Antibody Test-Introduction, Principle, Test Requirement, Test Procedure, Result-Interpretations,Test Result, Test Method, and Keynotes

Helicobacter pylori Antibody Test-Test Device, pouch, diluent and run test step

Introduction of Helicobacter pylori Antibody Test The bacterium, Helicobacter pylori can invade our gut and infection due to this bacterium is one of the major causes of peptic ulcer. This happens when inflammation caused by H. pylori affects the mucus coating of stomach or duodenum, …

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Leptospira Test-Introduction, Principle, Test Requirement, Procedure, Test Result, Test Method, Result-Interpretation, and Keynotes

Leptospira Test-Rapid Test Device, Diluent, and Specimen

Introduction of Leptospira Rapid Diagnostic Test  Leptospira rapid diagnostic test is for both present and past infection status, immunoglobulin M (IgM) indicates present history whereas immunoglobulin G (IgG) indicates past as shown above picture. Advantage Lepto IgG/IgM Combo Rapid Test is a visual, rapid, sensitive, …

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RFT: Introduction, Test Result, Unit, Normal Range, Test Methods, and Sign and Symptoms

RFT- Introduction, Test Result, Unit, Normal Range, and Test Methods

Introduction of Renal function tests (RFT) Renal function test (RFT), also known as kidney function test is a group of tests applied to assess the functions of the kidney which is used to screen for, detect, evaluate, and monitor acute and chronic kidney diseases (CKD). …

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ESR Test: Introduction, Test Result, Unit, Test Method, Clinical Significance, and Keynotes

Wintrobe's Method of ESR Test

Introduction of ESR The ESR stands for erythrocyte sedimentation rate. It is the rate at which erythrocyte sediment and the ESR test is a nonspecific assay that only confirms the presence or absence of inflammatory activity in the body like infection, cancer, or an autoimmune …

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Scrub Typhus Antibody Test-Introduction, Principle, Test Requirements, Procedure, Result Interpretation, Test Result, Test Method, Limitations, and Keynotes

Scrub Typhus Antibody Test-Negative and Positive Results

Introduction of Scrub Typhus  Scrub typhus and typhoid are quite different but common thing is that both cover fever. Scrub typhus also known as bush typhus, is a disease caused by the intracellular parasite, Orientia ( formerly Rickettsia)  tsutsugamushi and typhoid by Salmonella enterica serotype Typhi. Both causative agents are bacteria. Scrub …

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Protein Creatinine Ration (PCR) Test of Urine-Test Result, Unit, Normal Range ,Testing Methods, and Keynotes

Protein Creatinine Ration (PCR) Test of Urine-Test Result, Unit, Normal Range and Testing Methods

Introduction of Protein Creatinine Ratio (PCR) A urine protein creatinine ratio (PCR) test gives information regarding the kidneys working status. It measures the levels of proteins and creatinine in urine. This urine test helps clinicians to diagnose kidney disease and monitor conditions that can damage …

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Coomb Test: Introduction, Types, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Clinical Significance

Coomb Test Reagents-Anti- D serum and Anti-human globulin (AHG) or Coomb’s reagent

Introduction of Coomb Test Direct Coomb Test (DCT) is a type of Coomb test also called the anti-globulin test. It is of two types-Direct Coomb’s test (DCT) and the Indirect Coomb’s test (ICT). DCT uses to detect sensitized red blood cells (RBCs) while ICT detects …

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Indirect Coomb Test (ICT): Introduction, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Clinical Significance

Indirect Coomb Test Observation

Introduction of  Indirect Coomb Test (ICT) The indirect Coomb Test is a type of Coomb’s test also called the anti-globulin test. It is of two types-Direct Coomb’s test (DCT) and the Indirect Coomb’s test (ICT). DCT uses to detect sensitized red blood cells (RBCs) while …

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Direct Coomb Test (DCT): Introduction, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Clinical Significance

Direct Coomb Test (DCT) Observation

Introduction of Direct Coomb Test (DCT) Direct Coomb Test (DCT) is a type of Coomb test also called the anti-globulin test. It is of two types-Direct Coomb’s test (DCT) and the Indirect Coomb’s test (ICT). DCT uses to detect sensitized red blood cells (RBCs) while …

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MR-VP Test: Introduction, Principle, Test Requirement, Procedure, Result- Interpretation, and Keynotes

Result and Interpretation of Voges Proskauer Test

Introduction of MR-VP Test MR-VP test stands for Methyl Red –Voges Proskauer Test. MR-VP test will be discussed in two tests separately. One is for Methyl Red (MR) test and while other is for Voges Proskauer (VP)test. Introduction of Methyl Red Test The Methyl Red test is …

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Voges Proskauer Test: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Positive and Negative Bacteria, and Limitations

Voges Proskauer Test Reagents, Negative and Positive Test Results of VP Tests

Voges-Proskauer (VP ) Test Voges Proskauer  test for identification to the species level of the following groups of organisms Principle of Voges Proskauer Test Organisms utilizing the butylene glycol pathway produce acetylmethylcarbinol (acetoin) and butanediol, neutral end products that raise the pH towards neutrality (pH …

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Methyl Red Test: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Positive and Negative Bacteria, and Limitations

Methyl Red Test-Positive and Negative Test Results

Introduction of Methyl Red Test The Methyl Red test is for enteric gram-negative rods, as part of identification to species level. Its short name is MR test. Principle of Methyl Red Test The methyl red (MR) test uses to determine if an organism is able …

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Escherichia hermanii: Introduction, Classification, Pathogenecity, Lab Diagnosis, Treatment, and Keynotes

Escherichia hermanii colony morphology on MacConkey agar

Introduction of Escherichia hermanii Escherichia hermannii is a gram-negative rod. This bacterium belongs to the family of Enterobacteriaceae. It was first described in 1982. It had been previously known as enteric group 11 of Escherichia coli but was later reclassified as a distinct species in …

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Auramine-Phenol Fluorochrome Staining: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation, Limitations, and Keynotes

Acid fast bacilli (AFB) of Mycobacterium tuberculosis in Auramine-phenol fluorochrome Staining

Introduction of Auramine-Phenol stain Auramine-Phenol is a fluorochrome stain used to visualize acid-fast structures of various microorganisms, especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores. Ziehl-Neelsen (hot), and Kinyoun (cold) are still widely used methods to detect acid-fast structures …

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Cryptosporidium: Introduction, Classification, Morphology, Pathogenicity Lab Diagnosis, Treatment, Prevention, and Keynotes

Oocyst of cryptosporidium parvum in Modified Kinyoun's acid-fast stain of stool microscopy

Introduction of Cryptosporidium Cryptosporidium is a coccidian parasite that causes diarrhea called Cryptosporidiosis. It was discovered by medical parasitologist, Ernest Edward in 1907. Human infection caused by Cryptosporidium parvum was seen in 1976. The clinically important species of this genus are C. parvum, C. mammals, …

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ADA (Adenosine Deaminase) Test: Introduction, Principle, Test Requirements, Procedure, Normal Range, and Clinical Significance

ADA (Adenosine Deaminase) Test Reading in Spectrophotometer

Introduction of ADA (Adenosine Deaminase) Test ADA (Adenosine Deaminase) Test is very useful for suspicious cases of tuberculosis, increased levels of ADA could facilitate diagnosis. ADA is an enzyme that primarily functions in our body in the development and maintenance of the immune system that catalyzes the …

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Biochemistry Tests: Introduction, Group of Tests, Description, and Keynotes

Introduction of Biochemistry Tests Biochemistry Tests are the most common clinical laboratory tests used by clinicians to diagnose the pathological conditions of patients. For example, some tests and their clinical significances are as follows- Group of Biochemistry Tests This grouping system has been brought into …

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Vibrio cholerae Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation and Keynotes

Vibrio cholerae Serotyping Procedure

Introduction of Vibrio cholerae Serotyping Vibrio Serotyping helps to differentiate serotypes of Vibrio. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, V.cholerae O1 serovar Inaba, V. cholerae O1 serovar …

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Haemophilus Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Limitations, and Keynotes

Use of bacitracin 10 U on chocolate agar for screening Haemophilus of sputum culture

Introduction of Haemophilus Serotyping Haemophilus Serotyping helps to differentiate serotypes of Haemophilus influenzae. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, encapsulated strains of Haemophilus influenzae may …

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Shigella Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, and Keynotes

Shigella polyvalent A, B and C2 for Shigella dysenteriae, Shigella flexneri, and Shigella boydii

Introduction of Shigella Serotyping Shigella Serotyping helps to differentiate serotypes of Shigella. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, Shigella flexneri serotype 1, 2, 2a, S. sonnei, and S. …

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Widal Test: Introduction, Principle, Test Requirement, Procedure, Result- Interpretation, and Limitations

Widal Test Positive Demonstration

Introduction of Widal Test The Widal test is an agglutination test employed in the serological diagnosis of enteric fever. It is also applicable in febrile agglutinin tests. The test is named after Georges Fernand Isidore Widal, a French physician and bacteriologist. Salmonella antibody starts appearing in the …

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Salmonella Serotyping: Introduction, Serotypes and Risk Factors of Salmonella Infection

Salmonella Antisera and serotype positive demonstration

Salmonella Serotyping Serotyping is a type of epidemiological marker. It works on the principle of agglutination. agglutination or clumping: Positive No agglutination or clumping: Negative On this basis, Salmonella can differentiate whether Salmonella enterica serotype  Typhi or Paratyphi and so on. Serotype    O antigen Salmonella enterica serotype  Typhi ( …

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ABO and Rh Typing: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Applications, and Keynotes

ABO and Rh Typing-Slide Method

Introduction of ABO and Rh Typing ABO blood grouping and Rh typing are the most common blood group systems. There are nearly 300 blood group systems so far discovered. Other than ABO and  Rh are MNS, Lutheran, Kell, Duffy, etc. Karl Landsteiner discovered ABO ( …

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Antinuclear Antibody (ANA)Test: Introduction, Principle, Procedure, Result Interpretation, Clinical Significance, and Keynotes

Antinuclear Antibody (ANA)Test- Introduction, Principle, Procedure, Result Interpretation, Clinical Significance and Keynotes

Introduction of Antinuclear Antibody (ANA)Test The antinuclear antibody (ANA)test is a type of blood test in which serum tested for autoantibodies ( SLE) directed against native deoxyribonucleic acid and other nuclear constituents are produced. It involves a variety of tissues and is associated with a wide range …

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Therapeutic Drugs: Introduction, List of Drugs, and Keynotes

Introduction of Therapeutic Drugs Therapeutic drugs are those drugs that are applicable in some critical clinical conditions such as digoxin (to treat various heart conditions) and phenytoin (an anti-seizure medication). Therapeutic Drug monitoring (TDM) is essential for those drugs since it is the clinical practice …

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Anti -TPO Test: Introduction, Test Result, Unit, Normal Range, Assaying Method, and Keynotes

Anti TPO Test-Result, Unit, Normal Range, and Assaying Method

Introduction Anti -TPO test is an autoantibody assay that is against thyroperoxidase (TPO) (antigen). TPO is an enzyme present in thyroid hormone formation, catalyzing the oxidation of iodide on tyrosine residues in thyroglobulin for the synthesis of triiodothyronine (T3) and thyroxine (tetraiodothyronine -T4). This enzyme …

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Lipid Profile Test (LPT): Introduction, Test Result, Unit, Normal Range, Testing Methods, Clinical Significance, and Keynotes

Lipid Profile Test (LPT)-Test Result, Unit, Normal Range, Testing Methods, and Clinical Significance.JPG

Introduction of Lipid Profile Test (LPT) Before discussing the lipid profile test (LPT), it is better to know about lipids. Lipid is the fat and fatty substance that is stored in blood and tissues and it is utilized by the body as a source of …

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HbA1C Test: Introduction, Test Result, Unit, Normal Range, Testing Method, Clinical Significance, and Keynotes

HbA1C blood test using Nycocard Reader

Introduction of HbA1C Test HbA1c refers to glycated hemoglobin (HbA1c) which shows average plasma glucose concentration. It develops when hemoglobin, a protein within red blood cells (RBCs) that carries oxygen throughout the body, attaches to glucose in the blood, becoming ‘glycated’. By testing HbA1c, clinicians …

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Ferritin Test: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Ferritin Level- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Ferritin Test A serum ferritin test measures the level of ferritin in blood and it is a blood protein that contains iron. It will not be wrong to say that it is an indicator of how much iron the body stores. A low …

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GeneXpert Test For Tuberculosis: Introduction, Principle, Test Requirements, Test Procedure, Result -Interpretation, Advantages, Disadvantage, and Keynotes

GeneXpert Test For Tuberculosis-Mycobacterium tuberculosis (MTB) Detected High

Introduction of GeneXpert Test GeneXpert test is a molecular test for tuberculosis diagnosis by detecting the presence of  Mycobacterium tuberculosis (bacteria), as well as testing for resistance to the drug rifampicin. Principle of GeneXpert Test GeneXpert test is a highly sensitive and specific test for tuberculosis(TB) diagnosis that detects DNA sequences …

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Anti-Mullerian Hormone (AMH) Test: Introduction, Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Anti-Mullerian Hormone Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Anti-Mullerian Hormone (AMH) Test An Anti-Mullerian Hormone (AMH) Test measures the level of AMH in the blood which corresponds to egg count in females. It also acts as an indicator of a woman’s reproductive health. Applications of this hormone are as follows- Anti-Mullerian …

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PT, INR, and APTT Test: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

PT, INR, and APTT -Test Result, Unit, Normal Range, and Test Method

Introduction The PT, INR, and APTT tests are the common coagulation profile test. The prothrombin time (PT) test measures how rapidly blood clots. The international normalized ratio (INR) calculation assists to ensure that prothrombin time (PT) test results are standardized and accurate. The activated partial …

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Prolactin Blood Test: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Prolactin Level- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Prolactin Blood Test A prolactin (PRL) blood test or prolactin test or PRL test measures the level of prolactin in the blood and it is a hormone synthesized by the pituitary gland. This hormone is responsible for the breasts to grow and make …

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Dengue Fever Rapid Panel Test: Introduction, Test Result, Test Method, and Keynotes

Dengue Fever Rapid Panel- Test Result and Test Method

Introduction of Dengue Fever Rapid Panel Test Dengue Fever Rapid Panel Test is clear from the topic name that contains Dengue immunoglobulin M (IgM), Dengue immunoglobulin G (IgG), and Dengue nonstructural protein 1 (NS1) antigen tests. Blood is used for the assay and there is …

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Precalcitonin (PCT) Test: Introduction, Test Result, Unit, Normal Range, Test Method, and Keynotes

Precalcitonin- Test Result, Unit, Normal Range , and Test Method

Introduction of Precalcitonin (PCT) Test A precalcitonin (PCT) test measures the level of blood procalcitonin and it is normally present at a very low level. Our body will release procalcitonin into the bloodstream when there is a serious bacterial infection that acts as an indicator …

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Urinary Tract Infection Caused by Kluyvera intermedia: Patient History, Introduction of Pathogen, Lab Diagnosis, AST Pattern, and Keynotes

Gram Negative Rods of Kluyvera intermedia in Gram Staining of Culture

Patient History of Urinary Tract Infection Caused by Kluyvera An 80- year- old man with painful urination was requested for testing Urine Routine Examination (R/E), Complete Blood Cell (CBC) count, Thyroid stimulating hormone (TSH), Prostate-specific antigen (PSA), Liver function test (LFT), Renal function test (RFT), …

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Estimated glomerular filtration rate (eGFR): Introduction, Test Result, Normal Range, Unit, Test Methods, Clinical Significance, and Keynotes

Estimated glomerular filtration rate (eGFR)-Test Result, Normal Range, Unit, Test Methods , and Clinical Significance

Introduction of Estimated glomerular filtration rate (eGFR) The estimated glomerular filtration rate (eGFR) is an indicator that helps to know how well your kidneys are functioning. It is an estimated value based on a blood test and age, sex, and body type. Estimated glomerular filtration …

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Parasitic Infection Lab Diagnosis: Introduction, Importance, and Applications

Introduction and importance of  parasitic infections lab diagnosis  The parasitic infection has also emerged as a public health issue, especially in developing countries. Correct laboratory diagnosis of such parasitic infections (Parasitic Infections Lab Diagnosis)  not only helps in the early treatment of the diseases but also helps …

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Vitamin-B12: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Vitamin-B12 Level- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Vitamin-B12 Vitamin B12 is naturally present in animal foods like fish, meat, poultry, egg, milk, and other dairy product. It is also available in foods or supplements. It requires the formation of red blood cells (RBCs) and DNA which play a key role …

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Thyroid Function Test (TFT)-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Thyroid Function Test (TFT)- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Thyroid Function Test (TFT) If you are suffering from any of the symptoms stated in the table (Differences between Hypothyroidism and Hyperthyroidism), ask for a thyroid function test (TFT). Hypothyroidism is a condition in which the thyroid-stimulating hormone (TSH) level in your body …

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Occult Blood and Helicobacter pylori Antigen Stool Test- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Occult Blood and Helicobacter pylori Antigen Stool Test- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Occult Blood and Helicobacter pylori Antigen Stool Test Occult Blood and Helicobacter pylori Antigen Stool Tests are clinically important for the diagnosis of diseases. Fecal occult blood test positive is common in hookworm infestation. H. pylori antigen stool test is clear from the …

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Decarboxylase Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Decarboxylase Test-Positive and Negative Results Demonstration

Principle of Decarboxylase-Dihydrolase Test Lysine, ornithine, and arginine are the most common amino acids which use to detect an organism’s ability to decarboxylate or hydrolyze an amino acid in a decarboxylase medium, forming an amine that produces an alkaline pH. The basal medium for the …

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Methyl-α-D-glucopyranoside (MGP) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Methyl-α-D-glucopyranoside (MGP) Test- Positive and Negative Results Demonstration

Introduction of Methyl-α-D-glucopyranoside (MGP)Test Methyl-α-D-glucopyranoside (MGP) test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliflavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from  Enterococcus gallinarum and Enterococcus casseliflavus, while not important for purposes of control of …

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Indoxyl Acetate Disk Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Indoxyl Acetate Disk Test-Positive and Negative Results

Introduction of Indoxyl Acetate Disk Test  Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …

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Hippurate Hydrolysis Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Hippurate Hydrolysis Test-Positive and Negative Results

Introduction of Hippurate Hydrolysis Test The Hippurate Hydrolysis Test is applied to determine the ability of the organism to produce hippuricase which hydrolyzes the substrate hippurate. It is useful in the identification of Streptococcus agalactiae, Campylobacter jejuni, Listeria monocytogenes, and Gardnerella vaginalis.  Principle of the Test The end products …

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LAP (Leucine Aminopeptidase) Test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Limitations

 LAP (Leucine Aminopeptidase) Test-Positive and Negative Bacteria

Introduction of LAP (Leucine Aminopeptidase) Test LAP stands for leucine aminopeptidase which is an enzyme useful for definitive identification of catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group like  Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive). Principle of LAP test Hydrolysis of the leucine-pnaphthylamide substrate by …

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Microbiology Reporting Methods: Introduction, List of Templates, and Keynotes

Salmonella Typhi isolated from blood culture-Growth on Nutrient agar, blood agar and MacConkey medium, Biochemical reactions in TSI, SIM,Citrate and Urea agar, and growth on broth medium (blood culture bottle)-demonstration

Introduction of Microbiology Reporting Methods Microbiology Reporting Methods are useful to provide similar report formats from different Microbiology Laboratory platforms that should sound Microbiologically clear and concise. It should also be compatible with International Guidelines of Clinical Microbiology Practice like the Clinical & Laboratory Standards …

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XLD Agar: Introduction, Composition, Principle, Preparation, Test Requirements, Procedure, Colony Characteristics, Modifications, Uses, and Limitations

Salmonella Typhi (black colony) and Shigella growth on xylose lysine deoxycholate (XLD) agar

Introduction of XLD Agar XLD agar is a selective, differential, and indicator medium for the isolation of enteric pathogens. XLD stands for xylose lysine deoxycholate. It also supports the growth of more fastidious enteric organisms. XLD agar was developed by Taylor. It was specially designed to allow the …

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Mannitol Salt Agar (MSA): Introduction, Composition, Principle, Test Requirement, Procedure, Result -Interpretation, Limitation, and Keynotes

Staphylococcus aureus , CoNS and inhibited growth of Escherichia coli on MSA

Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify S. aureus from clinical and non-clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective is due to very high salt (7.5%) compared with other media. Gram-Positive Staphylococcus: …

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TCBS Agar : Introduction, Composition,Principle, Test Requirement,Procedure, Result -Interpretation, Limitation, and Keynotes

Vibrio cholerae colony morphology on TCBS agar

Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and Thiosulfate Citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar …

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Czapek Dox Agar: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

Aspergillus flavus growth on Czapek Dox Agar

Introduction of Czapek Dox Agar Czapek medium also called Czapek’s agar (CZA) from the surname of inventor Czech botanist Friedrich Johann Franz Czapek (1902-1903). Later CZA was modified by another American inventor, chemist Arthur Wayland Dox  (1910) and thus its name became’ Czapek-Dox medium’ and formula prepared …

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Dermatophyte Test Medium (DTM): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications,Uses, and Keynotes

Dermatophytes growth on Dermatophyte Test Medium (DTM)

Introduction of Dermatophyte Test Medium (DTM) Dermatophyte Test Medium (DTM) is a selective and differential medium recommended for the cultivation and isolation of pathogenic dermatophytes. The Dermatophytes are a distinct group of fungi that infect the hair, skin, and nails in humans producing a variety of …

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Potato Dextrose Agar (PDA): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

A. fumigatus growth on PDA

Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA) is a fungal medium and its ingredients are clear from its name it contains potato ( vegetable), dextrose ( sugar), and agar ( solidifying agent). PDA  is recommended for the isolation and enumeration of fungi …

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AST Pattern of Bacteria: Introduction, Group of AST Pattern, List of Antimicrobial Agents, and Keynotes

Antibiotic Susceptibility Testing (AST)-Kirby-Bauer Disk Diffusion Method

AST Pattern of Bacteria AST Pattern of Bacteria covers common bacterial pathogens groups like Enterobacteriaceae, Staphylococcus, Enterococcus, Pseudomonas aeruginosa, Streptococcus pneumoniae, beta-haemolytic streptococci (Streptococcus pyogenes, Streptococcus agalactiae), Haemophilus species, Acinetobacter, Viridans group of streptococci, Shigella, and Neisseria species. Enterobacteriaceae Antimicrobial Agents- Zone Diameter (Intermediate) Extra …

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Microbiology Reporting Templates: Introduction, List of Templates, Uses, and Keynotes

Pseudomonas aeruginosa Antimicrobial sensitivity testing Pattern

Introduction of Microbiology Reporting Templates is collected to reduce the time and similarity for beneficiaries. This is a collection of templates regarding branches of microbiology like bacteriology, mycology, parasitology, virology, and immunology but most commonly bacteriology. List of Microbiology Reporting Templates Culture Negative Reports Positive …

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Microbial Diagnostic Disks: Introduction, List of Disks, Uses, and Related Footages

Optochin Susceptibility Test

Introduction of Microbial Diagnostic Disks Microbial Diagnostic Disks make it easy to presumptive identification of organisms. They are simple and easy to handle for the identification of bacteria in the Clinical Microbiology Laboratory. Some common examples of such disks are optochin, oxidase, and bacitracin. List …

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Gram Negative Bacteria Biochemical Test: Table, Introduction, Common Tests with Short Description, List of Organisms, and Related Pictures

Biochemical Test of Common Gram Negative Bacteria

Common Gram Negative Bacteria Biochemical Test Table Introduction of Gram-Negative Bacteria Biochemical Test ‘Common Gram Negative Bacteria Biochemical Test’ is a genuine puny collection of clinically relevant bacteria isolated in the clinical bacteriology laboratory by the creator and those etiological agents captured in his camera …

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Butyrate Disk Test: Introduction, Principle,Test Requirements, Procedure, Result-Interpretation, Keynotes, and Limitations

Butyrate Disk Test-Positive

Introduction of Butyrate Disk Test  Butyrate disk test is useful to differentiate Moraxella catarrhalis from Neisseria species like Neisseria gonorrhoeae, Neisseria lactamica. Principle of Butyrate Disk Test  The butyrate disk test is a rapid test for the detection of the enzyme butyrate esterase. Bromochloro-indolyl butyrate (IB)  is the substrate. Hydrolysis …

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L-pyrrolidinyl-β-naphthylamide (PYR) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretations, and Limitations

L-pyrrolidinyl-β-naphthylamide (PYR) Test-Positive

Introduction of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) test uses to identify the capability of organisms utilizing substrate, PYR by L-pyrroglutamyl amino-peptidase. Application of this test are as follows: Principle of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) acts as a substrate for the detection of the enzyme …

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API Test for Bacteria: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation, Advantages, and Keynotes

API Test - Bacteria, Strip, Reagents, Reading Sheet and Log Book

Introduction of API Test for Bacteria API test for bacteria as shown above picture is a 20-jumbo tests kit for a biochemical panel for the identification and differentiation of members of the family Enterobacteriaceae.  API stands for Analytical Profile Index. It is an API 20E Test kit …

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Bile Solubility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Bile Solubility Test- Positive and Negative

Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …

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Malonate Test: Introduction, Composition of Medium, Principle, Procedure, Uses, Result-Interpretation, and Limitations

Malonate Test Positive and Negative bacteria

Introduction of Malonate Test Malonate test uses to check the ability of the organism to utilize malonate as the sole source of carbon for energy. To differentiate bacteria on the basis of a malonate utilization test. It is a colorful test to check the ability of …

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Optochin Susceptibility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Optochin Susceptibility Test-Positive

Optochin Susceptibility Test Optochin Susceptibility Test uses for presumptive identification of Streptococcus pneumoniae from viridans streptococci.  Principle of Optochin Susceptibility Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low concentration (5ug/ml). Optochin may also inhibit other viridians streptococci, but only …

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CAMP Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation,and Keynotes

CAMP Test-Positive

Introduction of CAMP Test  The CAMP test uses to identify preemptively Streptococcus agalactiae. It was first described in 1944 by Christie, Atkins, and Munch-Petersen, and the CAMP test is an acronym for their names. Principle of CAMP Test The beta lysin produced by Staphylococcus aureus is sphingomyelinase in nature, …

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Acetate Utilization Test: Introduction, Composition, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Keynotes

Acetate Utilization Test-Negative (left) and Positive (right side)

Introduction of Acetate Utilization Test Acetate Utilization Test ueses to determine the ability of an organism to use acetate as the sole source of carbon same as in the citrate utilization test for Enterobacteriaceae. It is the recommended assay for the differentiation of Shigella species from Escherichia coli. Composition of …

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Corn Meal Agar (CMA): Introduction, Composition, Principle, Test Requirements, Test Procedure, Result-Interpretation, Colony Characteristics, Uses, and Keynotes

Colony Morphology of Aspergillus flavus on cornmeal agar (CMA) demonstration

Introduction of Corn Meal Agar (CMA) Corn Meal Agar (CMA) is a well-established fungal medium that is a suitable substrate for chlamydospore production by Candida albicans and the maintenance of fungal stock cultures. This is a very simple formulation containing only cornmeal infusion and agar. The addition of glucose …

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Oxidation-Fermentation (OF) Test: Introduction, Composition of the medium, Test Requirements, Principle, Procedure, Result -Interpretation, and Limitations

Fermentation Test Positive and Negative Results of Bacteria

Oxidation-Fermentation (OF) Test Introduction Hugh and Leifson developed an Oxidation-Fermentation (OF) basal medium in 1953. It is a recommended medium for the detection of oxidation or fermentation of carbohydrates by bacteria. This basal medium is useful to aid in the identification of gram-negative bacteria on …

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Nitrate or Nitrite Reduction Test: Introduction, Composition of Medium, Principle, Test Requirements, Procedure, Result -Interpretation, and Limitations

Nitrate or Nitrite Reduction Test

Introduction of Nitrate or Nitrite Reduction Test The nitrate or nitrite reduction test uses to determine the ability of an organism to reduce nitrate to nitrite. Anaerobic metabolism requires an electron acceptor other than atmospheric oxygen. Many gram-negative bacteria use nitrate as the final electron acceptor. The …

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DNase Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result -Interpretations, and Limitations

Deoxyribonuclease (DNase) Test Positive and Negative Bacteria

Introduction of DNase Test for Bacteria DNase tests positive and negative bacteria as shown below image. This test is useful for the presumptive identification of Staphylococcus aureus which produces the enzyme deoxyribonuclease (DNase) from other Staphylococci which do not produce DNase. This test is also positive in the following …

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ONPG Test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

ONPG Test Negative (left) and Positive Bacteria (right)

Introduction of ONPG Test ONPG test is useful in differentiating members of the family  Enterobacteriaceae and genera Neisseria based on b-D-galactosidase activity. ONPG stands for o-Nitrophenyl-b-D-galactopyranoside. Here we discuss on ONPG Disk Test which is simple and easy to perform. Principle The ability of an organism to ferment lactose …

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MIU Test: Introduction, Principle, Composition, MIU Agar Preparation, Test Procedure, Result-Interpretation, and Keynotes

Motile and non-motile bacteria, indole positive and negative, urease positive and negative organisms on MIU agar demonstration

Introduction of MIU Test MIU test stands for motility indole urease test and it is used for three tests among them one for motility of bacteria, second for indole formation, and third for urea hydrolyzation test of bacteria. This assay is useful for the identification …

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Laboratory Instruments and Equipment: Introduction, Name List, and Short Description

Laboratory Instruments and Equipment

Introduction of Laboratory Instruments and Equipment Laboratory Instruments and Equipment make laboratory work easy and fast. Here we discuss the instruments and equipment of the Department of Pathology and Laboratory Medicine that includes Clinical Haematology, Clinical Biochemistry, Histopathology and Cytology, Clinical Microbiology and Immunology, Blood …

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Culture Media: Introduction, Common Ingredients, Types, Preparation, and Use of Common Culture Medium, Keynotes, and Related Images

Culture media with variety of microbes-Micrococcus growth on nutrient agar (upper left), Lactose fermenting(Lf) and non-lactose fermenting (NLF) colonies of Gram negative bacteria colonies on MacConkey medium, Hameophilus influenzae colony morphology on chocolate agar(lower left), beta-hemolytic colonies of Staphylococcus aureus( middle), and Pseudomonas aeruginosa antibiogram pattern on Muller-Hinton agar(MHA)

Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …

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Aeromonas hydrophila: Introduction, Identification Features, Keynotes, and Aeromonas Footages

Aeromonas hydrophila growth on MacConkey agar

Introduction of Aeromonas hydrophila Aeromonas hydrophila is a gram-negative, facultative anaerobe, rod-shaped motile(due to single polar flagellum) bacterium without capsule and spore having a size from 0.3-1.0 µm wide by 1.0-3.5 µm long. It is generally found in the aquatic environment. Its presence can not …

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Enterobacter cloacae: Introduction, Identification Features, Keynotes, and Enterobacter Footages

Encapsulated strain of Enterobacter aerogenes on MacConkey medium

Introduction of Enterobacter cloacae Enterobacter cloacae is a facultative anaerobic Gram-negative bacterium of size 0.3-0.6 x 0.8-2.0 μm. It is lacking capsule and spore and it is motile due to flagella which is a member of the Enterobacteriaceae family. E. cloacae is the normal gut …

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Shigella flexneri: Introduction, Identification Features, Keynotes, and Shigella Footages

Shigella boydii colony morphology on XLD agar

Introduction of Shigella flexneri Shigella are Gram-negative bacilli measuring about 1-3 µm X 0.5 µm. They are non-motile, non-encapsulated, non-sporing, and non-acid-fast bacteria belonging to the family Enterobacteriaceae. They cause an infectious disease called Shigellosis. There are four species or serogroups based on biochemical and …

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Citrobacter koseri: Introduction, Identification Features, and Keynotes

Citrobacter koseri growth on CLED agar

Introduction of Citrobacter koseri Citrobacter koseri is previously known as Citrobacter diversus. It is a facultative anaerobe of 1.0 × 2.0–6.0 μm in size, motile, non-capsulated, and gram-negative bacillus of the family Enterobacteriaceae. Citrobacter koseri acts as etiological agents of various pathological conditions like urinary tract infections (UTIs), …

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Salmonella Paratyphi: Introduction, Identification Features, Keynotes, and Salmonella Footages

Salmonella Serovar Paratyphi growth on Nutrient agar, Blood agar, MacConkey medium, BHI broth and Biochemical reactions in TSI, SIM, citrate and urea agar

Introduction Salmonella Paratyphi (serovar) belongs to Enterobacteriaceae and thus is a Gram-negative bacterium causing mild gastroenteritis to a life-threatening bloodstream infection. It causes paratyphoid fever and it is restricted to humans. Proper sanitation and hygiene may decrease its infection chance. Travelers must be cautious during …

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Proteus vulgaris: Introduction, Identification Features, Keynotes, and Proteus Footages

Dienes phenomenon of Proteus vulgaris strains

The genus Proteus was discovered in 1885 by Hauser and it is also named after a Greek god.  Proteus is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, motile, non-sporing rod-shaped bacterium without capsule and having a size of 0.4–0.8 μm in diameter and …

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Complete Thyroid Package: Introduction, Related assays, and Keynotes

Complete Thyroid Package: Introduction, Related assays, and Keynotes

Introduction of Complete Thyroid Package Complete Thyroid Package is a group of clinical laboratory tests. It uses to say the effects of thyroid disorder (hypothyroidism or hyperthyroidism) on our body and their monitoring. Normal thyroid stimulating hormone (TSH) controls the basal metabolic rate of the …

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Mycology: Introduction, Structure, Classification of Medically Important Fungi, Fungal Infection, Prevention, and Control of Fungal Infections

Mycology: Introduction, Structure, Classification of Medically Important Fungi, Fungal Infection, Prevention, and Control of Fungal Infections

Introduction of Mycology Mycology is the science that deals with the study of fungi whereas medical mycology is the science that deals with the study of fungi that causes disease is called medical mycology. General Features- Yeast Unicellular formSize-Width: 1-5 µmLength: 5-30 µmShape-Commonly oval shape …

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Aspergillus: Introduction, Classification, Colony Morphology, Pathogenesis, Clinical Features, Lab Diagnosis, Treatment, and Prevention

Aspergillus niger Colony Morphology on Sabouraud Dextrose Agar (SDA) after 3 days of incubation

Introduction of Aspergillus The genus Aspergillus has more than 180 species, among them, 38  are responsible to cause disease (able to grow at 37◦C. They are common in the environment. Aspergillus species are emerging pathogens which are ubiquitous molds that infect immunocompetent ( rarely) and immunocompromised …

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Microbiology and More Gallery: Introduction, List of Photos, and Keynotes

Human Microbiome Agar Art

Introduction of Microbiology and More Gallery Microbiology and More Gallery is a random collection hub of microbes and laboratory medicine-related footage. This is the genuine work of the author. Most of the pictures are commonly those encountered by the laboratory personnel during their working period. …

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SARS-CoV-2: Introduction, Classification, Variant, Pathogenicity, Lab Diagnosis, Treatment, and Prevention

True curves of SARS CoV-2 genes in RT-PCR assay for confirmation of COVID-19

Introduction of SARS-CoV-2 SARS-CoV-2 is a positive-sense single-stranded RNA virus that causes coronavirus disease 2019 ( COVID -19). SARS-CoV-2 stands for severe acute respiratory syndrome coronavirus 2. On March 11, 2020, the World Health Organization (WHO) characterized COVID-19 as a pandemic. On March 11, 2020, …

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Obesity Package: Introduction, Related Laboratory Tests, and Keynotes

Collecting blood sample for Obesity Package

Introduction of Obesity Package Obesity is the condition of being grossly fat or overweight. Whether people are suffering fromit or not is confirmed via body mass index (BMI). The common clinical laboratory tests that are beneficialto assay in this scenario are mentioned in the laboratory …

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Dengue Serotyping Real-Time PCR Test: Introduction, Principle, Test Requirement, Procedure, Result-Interpretation, and Keynotes

Positive curve of Dengue serotype-1

Introduction of Dengue Serotyping Real-Time PCR Test Dengue is a mosquito (Aedes aegypti)-borne viral disease. It is a major health threat in the tropical and sub-tropical territories of the world, particularly in urban and semi-urban areas. Approximately 50-100 million individuals have been infected with dengue …

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Visiting Visa via Sponsorship: Introduction, Requirements, and Keynotes

Visiting Visa via Sponsorship: Introduction, Requirements, and Keynotes

Visiting Visa via Sponsorship Introduction If someone’s family member in Europe (particularly the Czech Republic), he/she wants to bring his family member through sponsorship various documents are mandatory. Some documents may vary according to the situation and relation. A visit visa is also called a …

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Dengue: Introduction, Serotypes, Symptoms, Severity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Dengue Patient with severe headache and fever on IV Paracetamol 1 gm

Introduction Dengue is a mosquito (Aedes aegypti)-borne viral disease. It is a major health threat in the tropical and sub-tropical territories of the world, particularly in urban and semi-urban areas. Approximately 50-100 million individuals have been infected with this fever annually (WHO data) and more …

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Diabetes Package (Female): Introduction, Related assays, and Keynotes

Diabetes Package (Female): Introduction, Related assays, and Keynotes

Introduction of Diabetes Package (Female) Diabetes Package (Female) is a combination of laboratory tests, radiological assays, and clinicians consultations regarding the worsening of health status in a female diabetic patient and this package will provide a clear concept for diabetic patients to control their diabetic …

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Hematoxylin and Eosin (H&E) Stain: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, and Keynotes

Hematoxylin and Eosin (H&E) Stain: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, and Keynotes

Introduction of Hematoxylin and Eosin Stain Hematoxylin and eosin stain is abbreviated as H&E stain. It is one of the principal tissue stains used in histopathology to find out cancer from a suspected biopsy. The stain was first introduced by A. Wissowzky in 1876. The cell nuclei …

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Clinical Hematology Test: Introduction, List of Tests, Specimen Requirement, Testing Method, and Keynotes

Clinical Hematology Test: Introduction, List of Tests, Specimen Requirement, Testing Method, and Keynotes

Introduction of Clinical Hematology Test Clinical Hematology is the branch of laboratory medicine pertaining to the study of blood, blood-forming organs, and blood diseases. Hematology tests include laboratory estimation of blood formation and blood disorders. e.g. Complete blood count (CBC)- A count of the total …

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Pap Test: Introduction, Principle, Staining Procedure, Result- Interpretation, and Keynotes

Pap Test Introduction, Principle, Staining Procedure, Result- Interpretation and Keynotes

Introduction of Pap Test Pap test is also known as Pap smear and Pap stain. Pap stain was named after the three letters of the surname of developer George Papanicolaou in 1942 and was routinely used for cytological stain. It is the most common staining …

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Child Complete Health Checkup Package: Introduction, Related Assays, and Keynotes

Child Complete Health Checkup Package: Introduction, Related Assays, and Keynotes

Introduction of Child Complete Health Checkup Package ‘Child Complete Health Checkup Package’ is a combination of both laboratories as well as radiological tests and clinicians (doctors’) consultations. This package will help to indicate the health status of a child. Related Assays of Child Complete Health …

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Exotoxins and Endotoxins: Introduction, Differences, and Keynotes

Exotoxins and Endotoxins: Introduction, Differences, and Keynotes

Introduction of Exotoxins and Endotoxins Numerous bacteria produce toxins, enzymes, and pigments. Toxins and enzymes play significant roles in pathogenicity. Toxins are of two types- Differences Between Exotoxins and Endotoxins The differences between exotoxins and endotoxins are as follows- S. No Exotoxins Endotoxins 1. Exotoxins …

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Bacterial Endotoxin Detection by Chromogenic Method: Introduction, Principle, Product Characteristics, and Keynotes

Bacterial Endotoxin Detection by Chromogenic Method: Introduction, Principle, Product Characteristics, and Keynotes

Introduction of Bacterial Endotoxin Detection by Chromogenic Method Bacterial Endotoxin Detection by Chromogenic Method uses for screening and diagnosis of Gram-negative bacterial infection with a low limit of detection is 1 pg/ml (0.005EU/ml). It also applies to the quantitative detection of endotoxin in pharmaceutical and …

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Fungus (1-3)-β-D-Glucan Test by Chromogenic Method: Introduction, Principle, Product Features, and Keynotes

Fungus (1-3)-β-D-Glucan Test by Chromogenic Method: Introduction, Principle, Product Features, and Keynotes

Introduction of Fungus (1-3)-β-D-Glucan Test by Chromogenic Method Fungus (1-3)-β-D-Glucan Test by Chromogenic Method is used for screening and diagnosis of invasive fungal infection. It is a major cell wall component of most pathogenic fungi, used as a biomarker for the diagnosis of the invasive …

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Candida Mannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes

Candida Mannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes

Introduction of Candida Mannan ELISA Detection Kit Candida Mannan ELISA Detection is used for the specific detection of Invasive Candidiasis and Candidemia. It works on the principle of competitive Enzyme-linked Immunosorbent Assay (ELISA). It is applied for the quantitative detection of Candida mannan antigen in …

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Aspergillus Galactomannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes

Aspergillus Galactomannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes

Introduction of Aspergillus Galactomannan ELISA Detection Kit Galactomannan (GM) is a polysaccharide that is present in the cell wall component of Aspergillus species. It is released into the blood during fungal growth in the tissue that can be detected by Enzyme-Linked Immunosorbent Assay (ELISA). Principle …

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Immunochromatography Analyzer In Mycology Laboratory: Introduction, Instrument Structure, Instrument Parameters, Detecting Tests, and Keynotes

Mycology Laboratory: Introduction, Instrument Structure, Instrument Parameters, Detecting Tests, and Keynotes

Introduction of Immunochromatography Analyzer In Mycology Laboratory Immunochromatography Analyzer In Mycology Laboratory is an immunochromatography strip test system based on the photoelectric detection principle which should support the use of reagents based on the Gold Immunochromatography principle. With the suitable paper strip based on the …

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Full-Automatic Chemiluminescence Immunoassay System (FACIS): Introduction, Instrument Features, Testing Parameters, and Keynotes

FACIS-1

Introduction of Automation System for Invasive Fungal Diagnostics/FACIS Full-Automatic Chemiluminescence Immunoassay System (FACIS) is an open system to detect the content of endotoxin, (1-3)-β-D-glucan and galactomannan in bronchoalveolar lavage (BAL) fluid or serum clinical samples. With both a photometry system and chemiluminescence system built in, …

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Nosocomial Infections and Their Common Causative Agents: Introduction, Types, List of Microbial Agents, and Keynotes

Acinetobacter baumannii Complex-the most common bacterial etiological agent of Nosocomial Infections

Introduction of Nosocomial Infections Nosocomial infections are also called hospital-acquired infections (HAI) that occur during hospital stay and microbes are the causative agent of such infections. Common Nosocomial Infections The most commonly occurring nosocomial infections are as follows- Microbial Etiological Agents of Common Nosocomial Infections …

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SARS‑CoV‑2 RNA Extraction using Biocomma Kit: Introduction, Reagent Preparation, Extraction Requirements, Procedure, and Keynotes

SARS‑CoV‑2 RNA Extraction using Biocomma Kit: Introduction, Reagent preparation, Extraction requirements, Procedure and Keynotes

Introduction of SARS‑CoV‑2 RNA Extraction using Biocomma Kit Biocomma nucleic acid (DNA/RNA) extraction kit is a Chinese product that is useful for RNA extraction systems for COVID-19 detection. It is applied for manual extraction and it is completed in four steps- A kit (for 50 …

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Dengue Rapid Test for IgG and IgM Detection: Introduction, Principle, Test Requirements, Procedure, Result Interpretation, and Limitations

Dengue Rapid Test for IgG and IgM Detection: Introduction, Principle, Test Requirements, Procedure, Result Interpretation and Limitations

Introduction of Dengue IgG and IgM Rapid Test Dengue rapid test (RDT) uses for the detection of immunoglobulin G(IgG) and immunoglobulin M (IgM) where IgM is responsible for the past while IgG is for present infection as shown above picture. Dengue is a mosquito-borne viral …

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Indole test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

Indole test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

Indole Production test This indole test is useful to identify the bacteria on the basis of indole production. The list of indole-positive organisms is- Principle of Indole test The ability of an organism to split Indole from the amino acid tryptophan is due to the presence of …

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Whole Body Checkup: Introduction, Related Assays, and Keynotes

Whole Body Checkup: Introduction, Related Assays, and Keynotes

Introduction of Whole Body Checkup The ‘Whole Body Checkup’ is beneficial from the following points of view- Related Assays Package Contents- A whole Body Checkup includes the following tests:-  Keynotes on Whole Body Checkup

Alkaptonuria: Introduction, Symptoms, Causes, Lab Diagnosis, Treatment, and Keynotes

Alkaptonuria: Introduction, Symptoms, Causes, Lab Diagnosis, and Treatment

Introduction Alkaptonuria is an enzyme deficiency, homogentisic dioxygenase (HGD) rare inherited disorder. It is used to break down a toxic substance called homogentisic acid. Excess of homogentisic acid causes bones and cartilage to become discolored and brittle which causes later osteoarthritis, especially in the spine …

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Robertson’s Cooked Meat (RCM) Medium: Introduction, Principle, Composition, Preparation, Test Procedure, Result Interpretation, Uses and Keynotes

Robertson’s Cooked Meat (RCM) Medium Showing Saccharolytic anaerobes(red) and Proteolytic anaerobe (black)

Introduction of Robertson’s Cooked Meat (RCM) Medium Robertson’s Cooked Meat (RCM) Medium is the recommended medium for the cultivation of anaerobic as well as aerobic bacteria. It is especially for pathogenic Clostridium species from clinical, food, and water samples. It can also be used as a maintenance medium …

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Urine Casts: Introduction, Identification Features, and Clinical Significance

Granular Cast in Urine Microscopy

Introductions of Common Urinary Casts Urine casts in urine routine examinations are completed in the following steps- First request the patient to bring a urine specimen. After the arrival of the specimen in the clinical laboratory, take nearly 5 ml of urine and centrifuge it. …

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Urine Crystals: Introduction, Identification Features, and Clinical Significance

Urate Crystals or Uric acid Crystals in Urine Microscopy

Introductions of Common Urinary Crystals Urinary crystals in urine during the routine examination can appear in the following steps- First request the patient to bring a urine sample. After the arrival of the sample/specimen in the clinical laboratory, take nearly 5 ml of urine. Centrifuge it …

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Hot air oven: Introduction, Principle, Structure, Handling Procedure, Sterilization control, Uses, Advantages, and Disadvantage

Structure of Hot Air Oven and Functions

Introduction of Hot air oven A hot air oven is the most common method of sterilization in the laboratory working on dry heat. Sterilization is the process of removing or destroying all microorganisms including viruses, bacteria, and their spores from the article or surface without …

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Autoclave: Introduction, Fundamental Structures, Principle, Types, Sterilization Control, Working Mechanisms, Uses, and Precautions

Autoclave: Introduction, Fundamental structures, Principle, Types, Sterilization Control, Working Mechanisms, Uses, and Precautions

Introduction of Autoclave An autoclave is the most common method of sterilization in the laboratory working on moist heat. Sterilization is the process of removing or destroying all microorganisms including viruses, bacteria, and their spores from the article or surface without destroying its quality and …

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Incubator: Introduction, Principle, Handling Procedure, Types, Uses, and Keynotes

Blood agar and chocolate agar incubated CO2 Incubator

Introduction of Incubator The incubator is an electrical device in which the incubation process is performed which conditions an enclosure to a predetermined temperature since it provides and maintains all artificial optimal conditions for the growth of microbial culture as well as cell and tissue …

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Brain heart infusion (BHI) Broth: Introduction, Composition, Principle, Preparation, Test Procedure, Result-Interpretation, Modifications, Uses, and Keynotes

Blood inoculated BHI broth after 4 days of incubation showing bacterial growth

Introduction of BHI Broth Brain Heart Infusion (BHI) broth is a highly nutritive medium and is thus useful for cultivating a wide variety of microorganisms like aerobic and anaerobic bacteria, yeasts, and molds.  BHI broth is commonly used in blood culture work and allied pathological …

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RF Test: Introduction, Principle, Testing Procedure, Result -Interpretation, Normal Range, Clinical Significance, and Keynotes

RF Test- Negative Control (NC)-Negative, Positive Control (PC), Test-Positive

Introduction of RF Test RF test uses to detect autoantibody of rheumatoid factor which stands for rheumatoid factor. Rheumatoid arthritis ( RA) is a chronic systemic inflammatory disorder that may affect many tissues and organs like skin, blood vessels, heart, lungs, and muscles but particularly …

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ASO (Anti-streptococcal O)Test: Introduction, Principle, Procedure, Normal Range, Result- Interpretation, and Keynotes

ASO Test Result

Introduction of ASO Test ASO stands for Antistreptococcal O and it is an antibody targeted against streptolysin O, a toxic enzyme produced by group A Streptococcus bacterium. Antistreptococcal O and anti -DNase are the most common of several antibodies that are produced by the body’s immune system in response to a strep infection …

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Enzyme-Linked Immunosorbent Assay (ELISA): Introduction, Principle, Types, Procedure, Result-Interpretation, Advantage, Disadvantage, and Interfering Factors of ELISA Test Results

Enzyme-Linked Immunosorbent Assay(ELISA) Introduction, Principle, Types, Procedure, Result-Interpretation, Advantage, Disadvantage, and Interfering Factors of ELISA Test Results

Introduction of ELISA ELISA stands for Enzyme-Linked Immunosorbent Assay. It comes under antigen and antibody reaction tests and is useful for the identification of antigens or antibodies of the following specimens serum, urine, CSF, sputum, semen, supernatant of culture, stool, etc. It is also applicable …

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C-Reactive Protein (CRP) Test: Introduction, Principle, Testing Procedure, Result -Interpretation, Normal Range, Clinical Significance, and Other Methods

C-Reactive Protein (CRP) Test: Introduction, Principle, Testing Procedure, Result -Interpretation, Normal Range, Clinical Significance, and Other Methods of Testing

Introduction of C-Reactive Protein (CRP) Test C-Reactive Protein (CRP) is a carbohydrate-reactive protein. The C-Reactive Protein (CRP) Test was so named because it was first identified as a substance in the serum of patients with acute inflammation that reacted with the somatic ‘C’ carbohydrate antigen …

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Food Intolerance Package: Introduction, Related Lab Tests, and Keynotes

Fish Egg Pakoda Set with Red wine

Introduction of Food Intolerance Package The food Intolerance package includes a panel of tests to know if patients have sensitivity to a specific food. A food allergy test is also a part of the Food Intolerance package to know the reason for intolerance in which …

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RPR Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

RPR Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of Rapid Plasma Reagin (RPR) Test RPR is a screening non-specific test for syphilis caused by a bacterium, Treponema pallidum. It has replaced the VDRL test due to having properties like no need of preparing fresh reagents daily, no need for a microscope, and …

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TPHA Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

TPHA Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of TPHA Test TPHA test  stands for Treponema pallidum hemagglutination. This is a confirmatory test for syphilis that is caused by a bacterium, T. pallidum. TPHA test is a sensitive indirect hemagglutination test, that detects specific Treponema pallidum antibodies in serum within one hour. It uses …

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Master List of Record in Clinical Laboratory: Introduction, List, and Keynotes

Master List of Record in Clinical Laboratory: Introduction, List, and Keynotes

Introduction of Master List of Records in Clinical Laboratory ‘Master List of Records in Clinical Laboratory’ is an important document to reflect the quality of laboratory that is required during ISO accreditation as well as national accreditation. It is also required during laboratory renewable. It …

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VDRL Test: Introduction, Principle, Procedure, Result-Interpretation,Limitations, and Keynotes

VDRL Test Introduction,Principle, Procedure, Result-Interpretation,Limitations, and Keynotes

Introduction of VDRL Test VDRL stands for Veneral disease research laboratory. The laboratory was renamed the Treponemal Pathogenesis and Immunology Branch of the United States Public Health Service. The Venereal Disease Research Laboratory (VDRL) test is one of two variations of micro flocculation procedures used for serological testing …

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High-sensitivity cardiac troponin test (hs-cTnT): Introduction, Testing Conditions, Types, Clinical Significance, and Keynotes

High-sensitivity cardiac troponin test (hs-cTnT)-Introduction, Testing Conditions, Types, Clinical Significance, and Keynotes

Introduction of Troponin Test The high-sensitivity cardiac troponin test (hs-cTnT) is the new generation of heart/cardiac enzyme assaying that allows for the detection of very low levels of troponin T, assisting to diagnose heart attacks more quickly. It works on the principle of immunochromatography. It …

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Category A and B laboratory List in Nepal: Introduction, Laboratory Name, Address, License Number, Type, and Keynotes

Category A and B laboratory List in Nepal: Introduction, Laboratory Name, Address, License Number, Type, and Keynotes

Introduction of Category A and B laboratory List in Nepal There are two types of medical/clinical laboratories in Nepal. One is a government laboratory while another is a private laboratory. The private laboratory can not proceed with the tests without the accreditation of government bodies …

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Staphylococcus aureus: Introduction, Pathogenicity, Lab Diagnosis, Treatment, and Keynotes

Staphylococcus aureus: Introduction, Pathogenicity, Lab Diagnosis, Treatment, and Keynotes

Introduction of Staphylococcus aureus Scientific classification of Staphylococcus aureus is given as: Domain Bacteria Phylum Firmicutes Class Bacilli Order Bacillales Family Staphylococceae Genus Staphylococcus Species aureus Table: Biological Classification of Staphylococcus aureus Staphyle means bunch and Kokko mean berry. Staphylococcus aureus was isolated by Pasteur (1880) from a pus sample. …

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CLED Agar: Introduction, Composition, Principle, Preparation, Testing Procedure, Colony Characteristics, Modifications, Limitations, and Keynotes

Staphylococcus aureus (11 o'clock) Klebsiella pneumoniae (5 o'clock) and Pseudomonas aeruginosa (8 o'clock) growth on CLED agar-Back View

Introduction of CLED Agar CLED stands for cysteine, lactose, and electrolyte-deficient. CLED agar or medium is a differential culture medium used for the isolation and enumeration of bacteria in urine specimens from suspected cases of urinary tract infection (UTI). It favors the growth of all …

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Preventive Health Packages: Introduction, Types of Packages, and Keynotes

Preventive Health Packages: Introduction, Types of Packages, and Keynotes

Introduction of Preventive Health Packages ‘Preventive Health Packages’ are group tests and a combination of doctor check-ups for people. To know the health status and to save their lives and follow the quotation, ‘prevention is better than cure, these packages are really beneficial. A family …

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Radiology Department: Introduction, Staff, and Services

Radiology Department: Introduction, Staff and Services

Introduction Radiology Department or section is an integral part of any hospital for disease diagnosis or other health problems diagnosis. The most common is while X-ray advanced tool, Magnetic resonance imaging (MRI) helps clinicians to treat patients in various ways. Computerized tomography (CT)-scan is also …

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Well Women Check-up Package: Introduction, Investigation, and Keynotes

Well Women Check-up Package: Introduction, Investigation, and Keynotes

Introduction of Well Women Check-up Package ‘Well Women Check-up Package‘ is commonly advised the women who are aged 30 plus. This package is a combination of clinician/doctor and diagnostic tests to find out the health status of an individual. A family physician consultation will help …

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Advanced Senior Health Package: Introduction, Related assays, and Keynotes

Advanced Senior Health Package: Introduction, Related assays, and Keynotes

Introduction ‘Advanced Senior Health Package’ is a group test for senior citizens. This group of people is more prone to diseases/infections. To save their lives, do not ignore follow the quotation, ‘prevention is better than cure. This package is really beneficial. Advanced Senior Health Package …

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USG: Introduction, Uses, Centers in Kathmandu, Address, Contact Number, and Keynotes

Abdominal Scan-USG Footage

Introduction of USG USG stands for ultrasonography. It is also called an ultrasound scan and it is a common clinical test thatuses high-frequency sound waves to take live images from the inside of the body. It is also safe fromother imaging techniques due to lacking …

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Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …

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Phenylalanine Deaminase (PDA) Test: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and Limitations

Phenylalanine Deaminase(PDA) Test- Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and Limitations

Phenylalanine Deaminase (PDA) Test PDA stands for phenylalanine deaminase and PDA tests positive and negative are as shown below footage. It is used in the differentiation of gram-negative enteric bacilli based on the ability of the microorganisms to produce phenyl pyruvic acid by oxidative deamination. The deamination of …

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Double A Cafe: Introduction, Location, Facility, Services, Contact, and Keynotes

Double A Cafe-Front View of Counter

Introduction Double A Cafe (DAC) is situated in the heart of Kathmandu surrounded by Labim mall (Pulchowk), Krishna Mandir/Patan Durbar Square, Shri Bangalamukhi Temple, and Patan Doka touching Pimpahal Pokhari from the south and west directions. It is one of the perfect cafes for those …

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Hospitals in Kathmandu: Introduction, List of Hospitals, Locations, Description, Links, and Keynotes

Hospitals in Kathmandu: Introduction, List of Hospitals, Locations, Description, Links, and Keynotes

Introduction of Hospitals in Kathmandu Kathmandu is made up of three districts and they are Kathmandu, Lalitpur, and Bhaktapur, together with expanding an area of 899 square kilometers. It is the largest and busiest city in Nepal and thus has numerous reputed health care and …

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CT KUB: Introduction, Uses, Centers in Kathmandu, Address, Contact Number, and Website Links Keynotes

CT of the Kidneys, Ureters and Bladder (CT KUB)

Introduction of CT KUB A CT KUB stands for computerized tomography of the kidneys, ureters, and bladder. The use of the CT KUB scan is to obtain images from various angles of the urinary system and its surrounding structures, that enclose the area from the …

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CT IVU: Introduction, Uses, Centers in Kathmandu, Address, Contact Number, Website Links, and Keynotes

CT Urography or CTIVU Report

Introduction of CT IVU The full form of CT IVU is computed tomography-intravenous urography and it is also called CT urography. It is a technique that uses CT Scanners to take images of the genitourinary tract (organs like kidneys, ureters, and bladder). This examination or …

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Vitamin-D: Introduction, Normal Range in Blood, Daily Requirement, Clinical Significance, Symptoms, and Keynotes

Vitamin-D: Introduction, Normal Range in Blood, Daily Requirement, Clinical Significance, Symptoms, and Keynotes

Introduction of Vitamin-D Vitamin D is not a vitamin but a hormone or prohormone that is essential for maintaining healthy bones and teeth. It also helps in regulating inflammation, immune function, intestinal calcium absorption, and maintaining adequate blood levels of calcium and phosphorus, which are …

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Cholera: Introduction, Causative Agent, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Cholera: Introduction, Causative Agent, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Cholera is an infectious disease that is caused by the Gram-negative bacterium, Vibrio cholerae. It is responsible for severe watery diarrhea, which can lead to dehydration and even death if untreated. Cholera occurs due to eating food or drinking water contaminated V. cholerae. 13 …

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Health Benefits of Eating Fish: Introduction, Ingredients of Fish, Functions of Omega-3 Fatty Acids, and Keynotes

Health Benefits of Eating Fish: Introduction, Ingredients of Fish, Functions of Omega-3 Fatty Acids, and Keynotes

Introduction of Health Benefits of Eating Fish Fish is a part of a healthy diet since it has a low-fat high-quality protein with omega-3 fatty acids and vitamins (like vitamin D, and riboflavin i.e. B2). It is also rich in calcium and phosphorus and has …

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Thyroid Scan (Thyroid Scintigraphy): Introduction, Procedure, Uses, and Keynotes

Thyroid Scan (Thyroid Scintigraphy): Introduction, Procedure, Uses, and Keynotes

Introduction of Thyroid Scan A thyroid scan or thyroid scintigraphy is a diagnostic nuclear medicine assay that provides information regarding the structure and function of the thyroid gland and this technique makes it able to see the size, shape, function, and position of the gland. …

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HLA-B27 Test: Introduction, Result-Interpretation, Clinical Significance, and Keynotes

HLA-B27 Test- Introduction, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of HLA-B27 Test HLA-B27 stands for Human leukocyte antigen B27. It is a protein found on the surface of our white blood cells (WBCs) and it is a blood test that identifies HLA-B27 proteins. Although most human leukocyte antigens (HLAs) protect your body from …

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Glucose Tolerance Test(GTT): Introduction, Procedure, Result-Interpretation, and Keynotes

Glucose Tolerance Test(GTT): Introduction, Procedure, Result-Interpretation, and Keynotes

Introduction of Glucose Tolerance Test The glucose tolerance test (GTT), also known as the oral glucose tolerance test (OGTT) is useful to measure the body’s response to sugar (glucose)and it can be used to screen for type 2 diabetes. More commonly, a modified version of …

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Glucose Challenge Test (GCT): Introduction, Procedure, Result-Interpretation, and Keynotes

Glucose Challenge Test (GCT):Introduction, Procedure, Result-Interpretation, and Keynotes

Introduction of Glucose Challenge Test The glucose challenge test (GCT) measures the body’s response to glucose (sugar) and it is tested during pregnancy to screen for gestational diabetes (diabetes that develops during pregnancy). The GCT is done in two steps. Initially drink a glass of …

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PSA Test: Introduction, Normal Range, Prostate Cancer Symptoms, and Keynotes

PSA Test: Introduction, Normal Range, Prostate Cancer Symptoms, and Keynotes

Introduction of PSA Test PSA stands for prostate-specific antigen and it is a proteinous substance produced by prostate cells.It is a marker of prostate cancer. Its normal range in our blood is less than 4 nanograms. per milliliter. Range of PSA Test Normal Range: 4.0 …

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Thyroid Scan/Thyroid Computed Tomography Imaging: Introduction, Uses, Centers in Kathmandu, Address, Contact Number, and Keynotes

Thyroid Scan Footage and Report

Thyroid Scan Report Introduction Thyroid CT Scan/Thyroid Computed Tomography Imaging is a technique used to scan the thyroid gland that controls metabolism. It is a small, butterfly-shaped gland located in the middle of the lower neck having normal size of about 2 cm thick, 3 …

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Health Laboratory Establishment and Operation: Introduction, Space, Equipment, and Human Resource

Health Laboratory Establishment and Operation

Introduction of Health Laboratory Establishment and Operation As we know, ‘Laboratory Diagnosis’ is the third eye of doctors/clinicians that confirms the cause of pathology/ disease. It highlights the importance of Health Laboratory Establishment and Operation for better service. Here, we discuss in brief the national …

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HSG Test: Introduction, Uses, Centers in Kathmandu, Address, Contact Number, Website Links, and Keynotes

Introduction HSG stands for hysterosalpingography and it is an X-ray test to lay out the internal shape of the uterus and show whether the fallopian tubes are blocked. Uses of HSG Test Procedure of HSG A tiny tube is threaded through the vaginal and cervical …

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MRI Scan: Introduction, Uses, Centers in Kathmandu, Address, Contact Number, and Keynotes

Magnetic Resonance Imaging(MRI) Scan Setup

Introduction of MRI Scan MRI stands for magnetic resonance imaging and it uses magnetism, radio waves, and a computer to produce images of body structures. It provides essential information on various internal organs inside the body. It can be applied as a diagnostic tool to …

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DTPA Renal Scan: Introduction, Procedure Uses, Centers in Kathmandu, Address, Contact Number, and Keynotes

TC-DTPA Diuretic Renogram

Introduction DTPA stands for diethylenetriaminepentaacetic acid and it is an amino polycarboxylic acid consisting of a diethylenetriamine backbone with five carboxymethyl groups. DTPA Renal Scan is used to know how the left and right kidneys work comparatively. This test also recognizes any sites of blockages …

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CT- Scan: Introduction, Uses, Centers in Kathmandu, Address, Contact Number, and Keynotes

CT Scan

Introduction of CT -Scan CT stands for computed tomography and it is an imaging technique that combines a series of X-ray images taken at different angles and uses a computer to create images from these x-rays. It is widely available, and fast, and provides a …

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Gene Sequencing: Introduction, and Frequently Asked Questions (FAQs)

Cryptococcus Sequencing

Introduction of Gene Sequencing Deoxyribonucleic acid (DNA) sequencing is the process of finding out the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology (Sanger/Next-generation sequencing) that is used to find out the order of the four bases …

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Monkeypox Virus: Introduction, Classification, Incubation Period, Symptoms, Transmission, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction of Monkeypox Virus Monkeypox is a rare disease and its causative agent is the monkeypox virus (MPXV). This virus is a double-stranded DNA and it belongs to the Orthopoxvirus genus which includes the variola (smallpox) virus as well as the vaccinia virus, which is …

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Microbiology and Serology Laboratory: Introduction, List of Tests, Specimen,Testing Method, and Keynotes

Microbiology and Serology Laboratory: Introduction, List of Tests, Specimen,Testing Method, and Keynotes

Introduction of Microbiology and Serology Laboratory Here we are discussing not general microbiology but medical/clinical microbiology it is a discipline that encloses a wide range of testing methodologies, and it is complex in terms of organisms (bacteria, viruses, fungi, parasites) and methods used to isolate …

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Immunology Laboratory: Introduction, List of Tests, Specimen, Testing Method, and Keynotes

Introduction of Immunology Laboratory The Immunology Laboratory is useful for laboratory testing and clinical consultation in several broad domains including the evaluation of autoimmune disease, immunodeficiencies, immunoproliferative disorders, and allergies, as well as having responsibility for some senses of infectious disease serology. To provide absolute …

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Histopathology and Cytopathology Laboratory: Introduction, List of Tests, Specimen, Testing Method, and Keynotes

Histopathology and Cytopathology Laboratory: Introduction, List of Tests, Specimen, Testing Method, and Keynotes

Introduction of Histopathology and Cytopathology Laboratory Histopathology/histology is the branch of medical science that involves the examination of sampled whole tissues under a microscope. The size of the histopathological specimens is of three types, small, medium, and large. An autopsy is also called a post-mortem …

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MacConkey Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses, and Keynotes

MacConkey Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses, and Keynotes

Introduction of MacConkey Agar MacConkey agar (MAC) uses for the culture of gram-negative bacteria and therefore Enterobacteriaceae belonging bacteria grow well on this medium and coliforms also enjoy this medium. MAC is a modification of Neutral Red Bile Salt Agar developed by MacConkey. It was one of the …

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Laboratories in Nepal: Introduction, Category, Address, and Keynotes

Laboratories in Nepal: Introduction, Category, Address, and Keynotes

Introduction of Medical Laboratories in Nepal There are two types of medical/clinical laboratories in Nepal. One is a private laboratory while another is a government laboratory but both are monitored by National Public Health Laboratory (NPHL). NPHL is the government national reference laboratory under the …

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Molecular Diagnostics Laboratory: Introduction, List of Tests, Specimen,Testing Method, and Keynotes

Molecular Diagnostics Laboratory: Introduction, List of Tests, Specimen,Testing Method, and Keynotes

Introduction of Molecular Diagnostics Laboratory As we know, simple diseases are due to either metabolic disorders or infectious agents (bacteria, viruses, fungi, parasites, prions). Molecular Diagnostics is a powerful tool for the identification of both conditions at genetic levels (e.g. metabolic disorders like cancer, and …

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Cytogenetic Test: Introduction, List of Common Tests, Specimen Requirements, and Keynotes

Blood for Cytogenetic Test

Introduction of Cytogenetic Test The cytogenetic assay is useful for chromosome analysis, syndromes like Williams, Prader-Willi, Angelman, Miller-Dieke, Smith-Magenis, and DiGeorge, and hematological malignancies detection. List of Common Tests Test Method Application Karyotyping chromosome analysis e.g. neonatal Karyotyping, Product of Conception (POC) karyotyping Fluorescent InSitu …

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Importance of Clinical Lab Tests and Selection of the Best Laboratory

Importance of Clinical Lab Tests and Selection of the Best Laboratory

Importance of Clinical Lab Tests As we know, lab diagnosis is the third eye of clinicians/doctors. Medical/ clinical laboratory tests are important for us as a part of a routine checkup to look for changes in our health. These assays also assist to diagnose medical …

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Glucose Challenge Test (GCT): Introduction, Testing Time, Importance, patient Preparation, and Keynotes

Collected blood for Glucose Challenge Test (GCT)

Introduction of Glucose Challenge Test(GCT) The glucose challenge test (GCT) determines our body’s response to glucose (sugar). The GCT is done during pregnancy to screen for gestational diabetes that develops during pregnancy. GCT Testing Time It is generally tested between 24 and 28 weeks of …

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Serological Tests: Introduction, Some Common Serological Tests and Detection Methods

Blood samples for serological tests

Introduction of Serological Tests Serological tests also known as serology tests or antigen or antibody tests are any of several laboratory procedures carried out on blood specimens (the serum-an amber-colored, protein-rich fluid that separates out when blood coagulates) for the purpose of detecting antibodies or …

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Allergy Screening Test: Introduction, Result, Result-Interpretation, Clinical Significance, and Keynotes

Allergy Screening Test: Introduction,Result, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of Allergy Screening Test Allergy screening tests or Allergy blood tests are used to detect allergens and allergens are substances that can cause allergic reactions. In some people, the immune system recognizes allergens as foreign. As a consequence, the immune system reacts by producing a type …

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BMI (Body Mass Index): Introduction, Your Suggested Weight, and Keynotes

Introduction of BMI BMI stands for body mass index and it is a physical measurement applied to assess an individual’s total amount of body fat. It was invented by Belgian polymath Adolphe Quetelet in the 1800s and consequently is sometimes called the Quetelet index. The …

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Health Care Package: Introduction, Age-wise Packages, and List of Tests

Collecting blood specimen for General  Health Care package

Introduction of Health Care Package In this topic, we talk about the clinical diagnostic tests which indicate our health status. It is obvious that the health care package will depend on the individual age and pathological condition. Collecting blood specimens for General  Health Care package …

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Hypothyroidism and Hyperthyroidism: Introduction, Differences, and Keynotes

VITROS ECi (Immunodiagnostic)-automation for thyroid function test and more

Introduction of Hypothyroidism and Hyperthyroidism If you are suffering from any of the symptoms stated in the table, ask for a thyroid function test (TFT). Hypothyroidism is a condition in which the thyroid-stimulating hormone (TSH) level in your body is high than the normal range …

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Anaerobic Bacteria Culture: Introduction, Principle, Procedure, and Result Interpretation

Anaerobic Bacteria Culture: Introduction, Principle, Procedure, and Result Interpretation

Introduction of Anaerobic Bacteria Culture Obligate anaerobes can not grow in the presence of oxygen i.e. air. These organisms (obligate anaerobic bacteria) die rapidly on exposure to air, therefore for maintaining anaerobiosis various methods have been devised for anaerobic culture. Principle for Anaerobic Bacteria Culture/Cultivation …

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Probiotics: Introduction, List of Probiotics, Dose, Health Benefits, and Probiotics Products in Market

Probiotics: Introduction, List of Probiotics, Dose,Health Benefits, and Probiotics Products in Market

Introduction of Probiotics Probiotics are helpful to live microbes mainly bacteria and fungi (yeasts) that are good for our health, especially our digestive system. Their supplements and some foods are available in our market. For example yogurt and cereals. List of Probiotics The list of …

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Medical/Clinical Mycological Informations links: Introduction and List of Links

Medical or Clinical Mycological Informations links- Introduction and List of Links

Introduction of Medical/Clinical Mycological Information links Nearly 1.5 million environmental fungal species out of which 8000 plant pathogens and 300 human pathogens respectively but such scenarios have changed rapidly in recent years. Prolonged steroid use, recent neutropenia, diabetes mellitus, cancer (AML), and rheumatologic conditions are the …

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Biomedical Science and Research: Introduction, and Keynotes

Introduction of Biomedical Science and Research Biomedical science integrates the stream of biology and medicine in order to highlight the health of both animals and humans and research related to those. It helps how to maintain and promote health in humans and animals with knowledge …

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QuantiFERON-TB Gold (QFT) and Tuberculin Skin Test (TST): Introduction, Differences, and Keynotes

QuantiFERON-TB Gold (QFT) and Tuberculin Skin Test (TST): Introduction, Differences, and Keynotes

Introduction of QFT) and TST Interferon-Gamma Release Assays (IGRAs) are a newer blood test for tuberculosis that detects TB infection by measuring the release of interferon-gamma (IFN-g) from patient T cells after stimulation of a whole blood sample with highly specific TB antigens. QuantiFERON-TB Gold …

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IELTS: Introduction, Scoring Table, and Tips

Introduction of IELTS IELTS stands for International English Language Testing System which is developed to give a fair and accurate assessment of English language proficiency. Language specialists from Australia, Canada, New Zealand, the UK, and the USA help to develop IELTS questions. The test includes …

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Clinical Microbiology Laboratory Design: Introduction, Section Details, Special Features and, Keynotes

Clinical Microbiology Laboratory Design: Introduction, Section Details, Special Features and, Keynotes

Introduction of Clinical Microbiology Laboratory Design No doubt, developing ‘Clinical Microbiology Laboratory Design’ is really a hard task since the following factors affect it directly or indirectly- Sections Details Section 1 is the green zone and is fully safe. It has the following cabins- Section …

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Australian Universities/Institutions Having Scholarships: Introduction, List of University/Institutions

Australian Universities or Institutions Having Scholarships- Introduction, List of University or Institutions

Introduction of Australian University/Institutions Having Scholarships Australian Government Scholarships are available in top Australian Universities/Institutions for multiple undergraduates, Master’s, and Ph.D. degree programs. For more details, follow the list of Australian Universities/Institutions with links that host the Australia Awards Scholarships. List and Links of Australian …

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DNB and MD/MS: Introduction, Differences, and Keynotes

DNB Versus MD or MS- Introduction and Differences

Introduction of DNB and MD/MS DNB stands for Diplomate of National Board while MD for Doctor of Medicine and MS for Master in Surgery. Both (DNB and MD/MS) are postgraduate medical degrees and are equivalent to each other. For more clarity go through the differences …

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Fungal Disease: List, and Keynotes

Fungal Disease: List and Keynotes

List of Fungal Diseases A fungal infection is called mycosis. Even though most fungi are harmless to humans, some of them are etiological agents of fungal diseases under specific conditions like prolong steroid use, recent neutropenia, diabetes mellitus, cancer (AML), and rheumatologic conditions. . The …

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Microbes of Respiratory Infections: Introduction, List of Microbes, and Keynotes

Microbes of Respiratory Infections

Introduction for Microbes of Respiratory Infections The respiratory tract is a common site of infection since it comes in direct contact with the physical environment and is exposed to airborne microbes where a human takes about 20,000 breaths each day on average. Respiratory tract infection …

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SOP, Personal Statement, and Letter of Motivation: Introduction, Differences, and Keynotes

SOP vs Personal Statement vs Letter of Motivation

Introduction of SOP, Personal Statement, and Letter of Motivation SOP stands for statement of purpose and a letter related to it is a more generalized statement like a personal interview. A statement of purpose (SOP) explains why you want to study an offered subject topic …

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Microbes Responsible for Bloodstream Infections: Introduction, List of Microbes, and Keynotes

Microbes Responsible for Bloodstream Infections

Introduction of Microbes Responsible for Bloodstream Infections Bloodstream infections (BSIs) occur when microbes can enter the bloodstream as a severe complication of infections like pneumonia or meningitis, during surgery, or due to catheters and other foreign bodies invading the arteries or veins. Bloodstream infections (BSIs) …

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Seasonal flu and COVID-19: Introduction, Differences, and Keynotes

COVID-19 Molecular Diagnostics Laboratory

Introduction of Seasonal flu and COVID-19 Seasonal flu and COVID-19 both are contagious respiratory illnesses, but their causative agents are different viruses. The etiological agent of COVID-19 is severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) first identified in 2019 while flu is caused by infection …

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VITEK 2 Compact and VITEK MS: Introduction, Differences and Keynotes

VITEK 2 Compact System: Introduction, Testing Requirements, Testing Procedure, Result Interpretation and Keynotes

Introduction The VITEK 2 compact system is an automated microbiology bacteria and yeasts identification and antimicrobial susceptibility system which uses biochemicals for microbial identification whereas, VITEK MS performs the same function by covering a large number of microbes in a short time period at a …

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Auramine Staining: Introduction, Principle, Procedure, Result Interpretation, Limitations, Keynotes, and Related Footages

Auramine Staining: Introduction, Principle, Procedure, Result Interpretation, Limitations, Keynotes, and Related Footages

Introduction of Auramine Staining Auramine is a fluorochrome stain used to visualize acid-fast structures of various microorganisms especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores. Ziehl-Neelsen (hot), and Kinyoun (cold) are …

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