Aspergillus Galactomannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes — Fig. Aspergillus Galactomannan ELISA Detection Kit

Introduction of Aspergillus Galactomannan ELISA Detection Kit

Table of Contents

Galactomannan (GM) is a polysaccharide that is present in the cell wall component of Aspergillus species. It is released into the blood during fungal growth in the tissue that can be detected by Enzyme-Linked Immunosorbent Assay (ELISA).

Principle of ELISA

ELISA stands for Enzyme-Linked Immunosorbent Assay. It comes under antigen and antibody reaction tests and is useful for the identification of antigens or antibodies of following specimens serum and BAL (in the case of Mycology). It is also applicable for qualitative as well as quantitative determination of antigens or antibodies. In qualitative test determines antigen or antibody is present or absent whereas in quantitative determines the quantity of the antibody in titer and the titer is the highest dilution of the specimen usually serum which gives a positive reaction in the test. (For more details, follow the link: https://medicallabnotes.com/enzyme-linked-immunosorbent-assayelisa-introduction-principle-types-procedure-result-interpretation-advantage-disadvantage-and-interfering-factors-of-elisa-test-results/)

Aspergillus Galactomannan ELISA Detection Kit Characteristics

Product characteristics are described in a table-

	Serum	bronchoalveolar lavage (BAL)
Sensitivity	89.8%	92.4%
Specificity	90.7%	94.1%
Sample type	Serum, BAL
Sample volume	300 µL
Specification	96 tests/kit
Detection time	2 hours
Limit of Detection	0.5 ng/ml
Inter-assay variation	≤15%
Instrument	Microplate Reader

Table: Product Characteristics

Keynotes

FungiXpert® Aspergillus Galactomannan ELISA Detection Kit is a product of bio-state Inc.
Product advantages are as follows-

Advance
Accurate
Faster
Economical

Types of ELISA –

Direct ELISA (sandwich ELISA -For Ag detection),
Indirect ELISA (For Ab detection),
Competitive ELISA, and
Capture ELISA.

Direct ELISA is for the detection of antigens.
Indirect ELISA is for the detection of antibodies.
Sandwich or Competitive ELISA is for the detection of antigens.
The invalid test should be repeated.
FungiXpert® Aspergillus Galactomannan ELISA Detection Kit is a sandwich ELISA for the detection of Aspergillus galactomannan antigen in serum and BAL clinical specimens to help in the diagnosis of invasive aspergillosis.
FungiXpert® Aspergillus Galactomannan ELISA Detection Kit has the following benefits-

Rapid and accurate results within 2 hours
Convenient-Its reagents are ready-to-use.
Flexible: It has breakable strips that offer flexibility for Galactomannan immunoassay.

Interfering Factors of ELISA Test Results

The following factors which affect the ELISA result are as follow as-

Plate Assay: the shape and quality of the wells, the material of the plate, potential pre-activation, even or uneven coating
Buffer: pH, contamination
Capture and detection antibody: incubation time, temperature, specificity, titer, affinity
Blocking Buffer: cross-reactivity, concentration, contamination
Target antigen: conformation, stability, epitopes
Enzyme conjugate: type, concentration, function, cross-reactivity
Washes: contamination, frequency, volume, duration, composition
Substrate: quality/manufacturer
Detection: instrument-dependent factors
The reader or human error

Advantages of ELISA

Specific and sensitive with wide application
Equipment is cheap and easily available.
Reagents are “Cheap” and have a long shelf life.
Assays may be rapid.
Simultaneous assay, variety of labels
Potential for automation
No radiation hazards

Disadvantages of ELISA

Contamination
Expertise required to label and purify conjugates
Susceptible to interference from non-specific factors

Aspergillus Galactomannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes