Introduction
Table of Contents
Raoultella is a genus of gram-negative, non-motile, aerobic bacteria belonging to the Enterobacteriaceae family. The genus was named after Dr. Didier Raoult, a French microbiologist who made significant contributions to the study of Rickettsia. It was first described in 2001 as a distinct genus from Klebsiella, based on genetic and phenotypic differences.
Raoultella species are commonly found in soil, water, and various clinical samples, such as urine, blood, and wound swabs. They are opportunistic pathogens that can cause a range of infections, including urinary tract infections, pneumonia, and sepsis, particularly in immunocompromised individuals or patients with underlying health conditions.
There are currently five recognized species: R. planticola, R. ornithinolytica, R. terrigena, R. electrica, and R. variigena. R. planticola and R. ornithinolytica are the most commonly isolated species from clinical specimens, while R. terrigena is frequently found in environmental samples.
Raoultella species are generally susceptible to a range of antibiotics, although there have been reports of resistance to certain antibiotics, such as extended-spectrum beta-lactams and carbapenems. Accurate identification of species is important for appropriate antibiotic therapy and infection control measures.
Classification
Raoultella is a genus of gram-negative, non-motile, aerobic bacteria belonging to the Enterobacteriaceae family. The genus was formerly classified as part of the Klebsiella genus but was reclassified based on genetic and phenotypic differences.
There are currently five recognized species of Raoultella:
- Raoultella planticola: This species is commonly isolated from soil, water, and various clinical specimens. It has been associated with a range of infections, including urinary tract infections, wound infections, and bacteremia.
- Raoultella ornithinolytica: This species is frequently isolated from clinical specimens, particularly in patients with underlying health conditions. It has been associated with a range of infections, including urinary tract infections, pneumonia, and sepsis.
- Raoultella terrigena: This species is commonly found in soil and water, but has also been isolated from clinical specimens, particularly in immunocompromised individuals. It has been associated with a range of infections, including urinary tract infections, wound infections, and bacteremia.
- Raoultella electrica: This species was first isolated from an electrogenic biofilm and is notable for its ability to produce electricity.
- Raoultella variigena: This species is less commonly isolated from clinical specimens, but has been associated with a range of infections, including urinary tract infections and bacteremia.
Morphology
Raoultella are gram-negative bacteria with a rod-shaped or bacillus morphology. They are non-spore-forming, non-motile, and typically measure between 1-3 µm in length and 0.5-1 µm in width. They are facultative anaerobes, meaning that they can grow in both the presence and absence of oxygen. Its colonies on agar plates are smooth, circular, and convex with a shiny, mucoid appearance. They may appear slightly yellow or white in color, depending on the specific species and growth conditions, and are oxidase-negative and catalase-positive, meaning that they can break down hydrogen peroxide into water and oxygen.
Under the microscope, Raoultella have a characteristic appearance, with a peritrichous arrangement of flagella that radiates out from all sides of the cell. However, these flagella are generally not visible in gram-stained specimens. Instead, its cells stain gram-negative, meaning that they appear pink or red after staining with crystal violet and counterstaining with safranin.

Pathogenicity
Raoultella species are opportunistic pathogens that can cause a range of infections in humans, particularly in immunocompromised individuals or patients with underlying health conditions. The pathogenicity is attributed to its ability to produce a range of virulence factors, including adhesins, toxins, and enzymes.
Its species are commonly associated with urinary tract infections (UTIs), accounting for up to 8% of all UTI cases. They have also been reported to cause other infections, such as pneumonia, sepsis, wound infections, and bacteremia.
In addition to causing infections, they have been implicated in healthcare-associated outbreaks. For example, an outbreak of R. planticola infections was reported in a neonatal intensive care unit, where several infants developed sepsis and meningitis. Another outbreak of R. ornithinolytica infections was reported in a hospital ward, where several patients developed urinary tract infections.
Raoultella species have also been found to be associated with contaminated medical devices and environmental sources, such as water and soil. This highlights the importance of infection control measures and proper cleaning and disinfection practices in healthcare settings.
Lab Diagnosis
The laboratory diagnosis of Raoultella involves a combination of techniques to accurately identify the species and determine its antibiotic susceptibility.
- Specimen collection: Samples of blood, urine, sputum, wound exudates, or other relevant specimens are collected from the patient according to the site of infection.
- Microscopy: Gram staining of the specimen can reveal the presence of gram-negative rods. However, this technique cannot identify the species of Raoultella.
- Culture: The specimen is inoculated onto appropriate culture media such as blood agar, MacConkey agar, or CLED agar, and incubated aerobically at 37°C for 24 to 48 hours. Raoultella species typically grow as smooth, circular, and convex colonies with a shiny, mucoid appearance.
- Biochemical tests: Various biochemical tests such as oxidase test, catalase test, and API strips can be performed to identify the genus and species of the bacteria. For instance, Raoultella species are oxidase-negative and catalase-positive and can be identified by their characteristic API profiles.
- Antibiotic susceptibility testing: The susceptibility of the Raoultella isolate to antibiotics can be determined using various methods such as disk diffusion, broth dilution, or automated systems. The choice of antibiotics depends on the site of infection and the susceptibility profile of the organism.
- Molecular methods: Molecular methods such as polymerase chain reaction (PCR) and sequencing can be used to confirm the identification of Raoultella and to detect specific resistance genes.

Treatment and Prevention
The treatment of Raoultella infections depends on the specific species, site of infection, and antibiotic susceptibility profile of the organism. In general, they are susceptible to a range of antibiotics, including beta-lactams, fluoroquinolones, aminoglycosides, and trimethoprim-sulfamethoxazole. However, resistance to certain antibiotics, such as extended-spectrum beta-lactams and carbapenems, has been reported in some species of Raoultella. Therefore, antibiotic susceptibility testing is important to guide the choice of appropriate antibiotics.
Prevention of Raoultella infections involves implementing infection control measures in healthcare settings, such as proper hand hygiene, cleaning and disinfection of medical equipment, and appropriate use of antibiotics. In addition, careful selection and use of invasive medical devices, such as urinary catheters and central venous catheters, can help to reduce the risk of Raoultella infections.
Keynotes
- Overall, while Raoultella species are generally susceptible to a range of antibiotics, there have been reports of resistance to certain antibiotics, such as extended-spectrum beta-lactams and carbapenems. Accurate identification of Raoultella species and appropriate antibiotic therapy is essential for the effective management of infections caused by these bacteria.
- In summary, Raoultella is gram-negative, non-motile, rod-shaped bacteria with a smooth, mucoid appearance on agar plates. They are catalase-positive and have a characteristic peritrichous arrangement of flagella, although these are not visible in gram-stained specimens.
- Overall, the laboratory diagnosis of Raoultella requires a combination of techniques to accurately identify the species and determine its antibiotic susceptibility. The identification of Raoultella at the species level is important for appropriate antibiotic therapy and infection control measures.
- In summary, the treatment of Raoultella infections requires appropriate antibiotic therapy based on the species and antibiotic susceptibility profile of the organism. Prevention of Raoultella infections involves implementing infection control measures and appropriate use of antibiotics in healthcare settings.

Further Readings
- Scientific Journals and Research Articles:
- Search databases like PubMed, Google Scholar, or specialized microbiology journals for articles related to Raoultella. Look for studies on its taxonomy, epidemiology, clinical cases, antibiotic resistance, and more.
- Review Articles:
- Review articles can provide a comprehensive overview of the current knowledge about Raoultella. They often summarize existing research and highlight important findings. Search for review articles in microbiology and infectious disease journals.
- Microbiology Textbooks:
- Standard microbiology textbooks often dedicate sections to various bacterial genera. Look for well-regarded textbooks that cover Enterobacteriaceae or clinical microbiology to find information on Raoultella.
- Clinical Microbiology Resources:
- Medical and clinical microbiology resources may cover the clinical aspects of Raoultella infections, including diagnosis, treatment, and management. Check medical textbooks or online resources used by clinicians.
- Microbiology and Infectious Disease Conferences:
- Attending or reviewing presentations from microbiology and infectious disease conferences can provide insights into the latest research on Raoultella. Conference abstracts and proceedings often highlight ongoing research.
- Online Databases and Resources:
- Explore databases such as the National Center for Biotechnology Information (NCBI) GenBank for genetic information and sequences related to Raoultella species.
- Research Institutions and Universities:
- Academic institutions and research centers often publish studies on various bacterial species. Exploring the websites of renowned microbiology departments can lead you to relevant research.
- Microbiology Associations and Societies:
- Professional associations like the American Society for Microbiology (ASM) or the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) might provide resources or publications related to Raoultella.