Fungal Identification-Introduction, Conventional Method and MALDI TOF Method, Application, and Keynotes

Conidia, phialides(sterigmata), metullae, and conidiophore of Penicillum in LPCB preparation of culture microscopic examination

Introduction Fungal identification plays a vital role in diagnosing infections. Mycologists use various methods to identify fungal species. Firstly, conventional techniques rely on morphology and culture characteristics. Additionally, molecular assays enhance detection accuracy. Researchers now employ advanced tools to expedite fungal identification. Moreover, timely identification …

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Rose Bengal Agar (RBA)- Introduction, Principle, Composition, Test Procedure, Colony Characteristics, Uses, and Keynotes

Fungal growth on Rose Bengal Agar (RBA) at centre

Introduction Rose Bengal Agar (RBA) is a selective medium for isolating fungi. It contains rose bengal dye, which inhibits bacterial growth. This medium supports the growth of various fungi. Chloramphenicol in RBA further suppresses bacterial contamination. Scientists and clinicians use it for environmental and clinical …

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Comparative Analysis of Capsule Expression in Cryptococcus neoformans: India Ink Visualization from Clinical CSF Samples versus SDA Agar Cultures

Cryptococcus neoformans capsules in India Ink Preparation of Culture of organisms

Introduction of India Ink preparation Cryptococcus neoformans is an encapsulated yeast that causes cryptococcal meningitis, particularly in immunocompromised individuals. The capsule is a critical virulence factor, and its visualization is essential for diagnosis. This preparation is a common method used to visualize the capsule. Here, …

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Serratia liquefaciens: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Serratia liquefaciens and Klebsiella pneumoniae growth on CLED agar of Urine Culture

Introduction Serratia liquefaciens is a species of Gram-negative, rod-shaped bacteria belonging to the genus Serratia. This bacterium is known for its diverse distribution in various environmental niches, including water, soil, plants, and human-related habitats. It has also been associated with clinical infections in humans, making …

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Candida Chromagar: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Candida Chromagar: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Introduction Candida Chromagar is a selective and differential agar medium used for the isolation and identification of Candida species. HiMedia is a well-known manufacturer of microbiological culture media and laboratory products. Here are some keywords related to this agar: Principle The principle of Candida Chromagar …

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