Fungal Identification by NGS: Introduction, Principle, Test Methods, Test Procedure, Result-Interpretation, Clinical Significance, and Keynotes

PCR products of fungal DNA from pure culture after amplification with primers

Introduction Fungal identification by NGS (Next-Generation Sequencing) uses advanced DNA sequencing. Moreover, it rapidly detects diverse fungal species. It enhances diagnostic accuracy. Additionally, it supports personalized treatment decisions. Principle NGS sequences fungal DNA directly. Consequently, it generates millions of short DNA reads. Then, bioinformatics tools …

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Top 10 Fungal Pathogens: List with short Descriptions, and Footage

Mucor colony morphology on SDA

Top 10 Fungal Pathogens List A list of the Top 10 Fungal Pathogens is summarized as follows- Short Descriptions of Top 10 Fungal Pathogens Candida albicans Aspergillus fumigatus Cryptococcus neoformans Histoplasma capsulatum Blastomyces dermatitidis Coccidioides immitis (and C. posadasii) Pneumocystis jirovecii Mucor species (and Rhizopus …

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Soft Tissue Infections- Introduction, Types, Sign and Symptoms, Common Pathogens, Laboratory Diagnosis, Treatment, Prevention and Control, and Keynotes

 Introduction Soft tissue infections (STIs) refer to a broad category of infections that affect the body’s soft tissues, including muscles, skin, subcutaneous tissue, and connective tissues. These infections can be caused by various pathogens, including bacteria, viruses, fungi, and even parasites. They can range in …

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Pus Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Pus culture and sensitivity is a diagnostic test commonly performed in medical settings to identify and analyze the microorganisms present in a wound, abscess, or other sources of pus. Pus is a thick, yellowish or greenish fluid that accumulates at the site of infection …

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Ochroconis gallopava: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Conidiophores and conidia of Ochroconis gallopava in LPCB tease mount of culture microscopy

Introduction Ochroconis gallopava is a species of fungus belonging to the Ascomycota phylum. It is commonly known as the “riverbank grape black rot fungus.” This fungus is primarily known for its pathogenic properties, as it can cause significant damage to various plant species, particularly grapevines. …

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Candida parapsilosis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Candida parapsilosis growth on Candida CHROMagar

Introduction Candida parapsilosis is a species of yeast that belongs to the Candida genus, which includes various fungi commonly found in the environment and on the human body. Candida species are part of the normal human microbiota, residing on the skin, mucous membranes, and gastrointestinal …

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Candida tropicalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Various Candida species grew on Candida CHROMAgar -Candida albicans (green), Candida tropicalis (cream-colored to light pink colonies), Candida glabrata (purple to lavender-color), Candida krusei(dark blue to mauve colonies), and Candida parapsilosis (cream-colored) as shown in the figure.

Introduction Candida tropicalis is a species of yeast, belonging to the Candida genus, commonly found in the environment and on the human body as part of the normal microbial flora. Similar to other Candida species, it can exist as a commensal organism, coexisting harmlessly with …

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Candida glabrata: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Yeast cells and hyphae of Candida glabrata in Giemsa stained smear of sputum microscopy at a magnification of 1000X

Introduction Candida glabrata is a species of yeast, specifically a type of fungus, that belongs to the Candida genus. It is commonly found in the environment and is also part of the normal microbial flora on the skin and mucous membranes of humans. While it …

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