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Introduction of Vibrio cholerae Serotyping Vibrio Serotyping helps to differentiate serotypes of Vibrio. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, V.cholerae O1 serovar Inaba, V. cholerae O1 serovar …
Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and Thiosulfate Citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar …
Introduction of Exotoxins and Endotoxins Numerous bacteria produce toxins, enzymes, and pigments. Toxins and enzymes play significant roles in pathogenicity. Toxins are of two types- Differences Between Exotoxins and Endotoxins The differences between exotoxins and endotoxins are as follows- S. No Exotoxins Endotoxins 1. Exotoxins …
Introduction Cholera is an infectious disease that is caused by the Gram-negative bacterium, Vibrio cholerae. It is responsible for severe watery diarrhea, which can lead to dehydration and even death if untreated. Cholera occurs due to eating food or drinking water contaminated V. cholerae. 13 …
Introduction The genus, Vibrio is a member of the family, Vibrionaceae and the most common human pathogens are Vibrio cholerae, V. parahaemolyticus, and V. vulnificus. Vibrio cholerae is a species of Gram-negative, motile, non-sporing, and non-capsulated, facultative anaerobe and comma-shaped bacterium. It is a ubiquitous …
Introduction of Motility Test for Bacteria/ Hanging Drop Preparation Bacteria are motile due to having flagellum/flagella. On the flagella, bacteria are motile and non-motile and therefore some bacteria are motile (Vibrio cholerae, E. coli, Proteus species, etc.) whereas some others are non-motile like Klebsiella, Shigella, …
Introduction of String Test String test is a special test of bacteria to identify Vibrio cholerae (String test positive) from Vibrio species and other bacteria like Aeromonas species and Plesiomonas shigelloides. This assay can perform on a glass slide or plastic petri dish, suspending overnight …
Introduction of Oxidase Test (Kovac’s Method) Gordon and McLeod introduced the application of a dimethyl-phenylenediamine dihydrochloride solution to test for the presence of oxidase systems in 1928. In particular, they used the test to differentiate N. gonorrhoeae (oxidase test positive) from Staphylococcus species and Streptococcus species (oxidase negative). The sensitivity of the …