Fungal infections represent a growing global health challenge, causing over 1.7 million deaths annually, with nearly 75% of cases remaining…
Introduction Broad-range PCR assays for fungi are molecular diagnostic methods designed to detect a wide spectrum of fungal pathogens directly…
Introduction Sanger sequencing, developed by Frederick Sanger in 1977, is a chain-termination DNA sequencing method that remains the gold standard for molecular-level fungal…
Introduction Accurate identification of fungi is critical for clinical, environmental, and epidemiological purposes. Traditional culture and morphology-based methods are time-consuming…