All Notes

Biochemistry Test: Introduction, List of Tests, Unit and Normal Range

Introduction of Biochemistry Test

Biochemistry is the branch of medicine that deals with a series of blood tests used to evaluate the functional ability of several critical organs and systems of the body. e.g. renal function test (RFT) for kidney, liver function test (LFT) for liver, Cardiac profile test for the heart, thyroid function test for thyroid disorders, Calcium, phosphorus, Alkaline phosphatase, and uric acid for bones and joints, similarly for nutrition status protein, albumin, globulin, albumin, and globulin ratio are tested, while arterial blood gas analysis (ABG) is mainly applied to monitor carbon dioxide and oxygen levels related to pulmonary (lung) function but is also used to measure blood pH and bicarbonate levels for certain metabolic conditions, glucose tolerance test (GTT) involves repeated testing to determine the rate at which glucose is processed by the body.

Fig. Biochemistry tests results on the screen of Vitrous 250 analyzer

List of Tests

Glucose Test

  • Fasting
  • Post Prandial (PP)- 2 hours
  • Random
  • Oral Glucose Tolerance Test (OGTT)-75gm/100gm
  • Glucose Challenge Test (GCT)-50 gm

Renal Function test

  • Urea
  • Serum Creatinine
  • Sodium (Na+)
  • Potassium (K+)

Other tests

  • Total calcium
  • Serum inoragnic Calcium (iCa+)
  • Uric Acid
  • Glycated Haemoglobin (HbA1C)

Liver Function test

  • Total Bilirubin
  • Direct Bilirubin
  • Alkaline Phosphate
  • Serum Glutamic-Oxaloacetic Transaminase (SGOT) or Aspartate Aminotransferase(AST)
  • Serum Glutamic-Pyruvic Transaminase (SGPT) or Alanine Aminotransferase(ALT)
  • Total Protein
  • Albumin

Lipid Profile

  • Total Cholesterol
  • High-Density Lipoprotein (HDL)
  • low-density lipoproteins (LDL)
  • Triglyceride

Serum Lipase

Serum Amylase

Adenosine Deaminase (ADA ) Test for (serum/CSF/Fluid)

Unit and Normal Range

Glucose Test ValueUnitNormal Range (SI Unit)Conversion Factor
Fastingmmol/L3.5-6.1(X18=mg/dL)
PP (2hrs)mmol/L<8.3 (X18=mg/dL)
Randommmol/L3.8-7.8 (X18=mg/dL)
OGTT (75gm/100gm)mmol/L (X18=mg/dL)
GCT(50 gm)mmol/L<7.8 (X18=mg/dL)
Renal Function test
Ureammol/L1.6-7.0(X6=mg/dL)
S. Creatinineμmol/LMale: 60-130;Female:40-110(X0.01131=mg/dL)
SodiummEq/L135-148
Potassium mEq/L 3.8-5.0
Chloride mEq/L 60-150
Other Tests
Total calciummmol/L2.1-2.6(X4=mg/dL)
S.Calcium (iCa+) mmol/L (X4=mg/dL)
Uric Acidμmol/LMale:200-400; Female:140-340÷59.48=gm/ dl
HbA1C%5.7
Prediabetes: 5.7-6.4
Diabetes:≥6.5
Liver Function test
Total Bilirubinμmol/L3-21
Direct Bilirubinμmol/L0-5
Alkaline PhosphateU/L<306
SGOTU/L5-40
SGPT U/L 5-45
Total Proteingm/L60- 80÷10=gm/dl
Albumingm/L37-49÷10=gm/dl
Lipid Profile
Total Cholesterolmmol/L3.5-5.1(X38.7=mg/dL)
HDLmmol/L0.8-1.6(X38.7=mg/dL)
LDLmmol/Lupto 4(X38.7=mg/dL)
Triglyceridemmol/L0.5-1.8(X88.57=mg/dL)
S. LipaseU/L<60
S. AmylaseU/L28-100
ADA (serum/CSF/Fluid) U/L Seum<15,CSF<10;Fluid<40
Table: Unit and Normal Range of Biochemistry Tests

Contd…

Medical Lab Notes

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