Helicobacter pylori Antibody Test-Introduction, Principle, Test Requirement, Test Procedure, Result-Interpretations,Test Result, Test Method, and Keynotes

Introduction of Helicobacter pylori Antibody Test

The bacterium, Helicobacter pylori can invade our gut and infection due to this bacterium is one of the major causes of peptic ulcer. This happens when inflammation caused by H. pylori affects the mucus coating of stomach or duodenum, the first section of small intestine which leads to sores on the lining and is called peptic ulcer. If Helicobacter pylori Antibody Test is positive that indicates there may be fighting of H. pylori in our body.

Principle

H.pylori Antibody Test works on the principle of immunochromatography. H. pylori Ab Rapid Test is a sandwich lateral flow chromatographic immunoassay for the qualitative detection of antibodies (IgG, IgM, and IgA) against H. pylori in human serum, plasma, or whole blood.

Test Requirements

• Tes sample- Serum/plsma or whole blood

• Test kit provides-Individually sealed foil pouches containing-

1. One cassette device
2. One desiccant
3. Plastic droppers
4. Sample diluent
5. Test manual (instruction for use)

• Waste bin
• Gloves

Procedure

1. Add 2 drops ( approx.100 μL) of serum/plasma.
2. Add 2 drops of buffer.
3. Wait for the colored line/s.
4. Read the result at 15 minutes.

Result-Interpretation

• Only one band at control region-Negative
• Both bands at control and test regions: Positive
• No band at all or band at only test region: Test invalid and in this sceanrio, repeat the test with another frseh test device.

H. pylori Antibody Test-Test Result, and Test Method

Keynotes

• H. pylori is a leading cause of peptic ulcers, but these ulcers may also develop from other reasons like using too many nonsteroidal anti-inflammatory drugs like ibuprofen.

• Features of H. pylori Ab Combo Rapid Test are as follows-

1. Uses serum, plasma, or whole blood specimen.
2. There is no need to avoid certain medications.
3. The test procedure is non-invasive, easy-to-perform.
4. The time to result is 15 minutes.

• H-PYLORI ANTIBODY (IgG/IgM) CARD Test is a qualitative detection of Helicobacter pylori IgG/IgM/IgA antibodies in human serum and plasma.

Further Readings

• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576260/
• https://www.btnx.com/Product?id=1928
• https://www.mayoclinic.org/diseases-conditions/h-pylori/diagnosis-treatment/drc-20356177