Microbiology Reporting Templates: Introduction, List of Templates, Uses, and Keynotes

Introduction of Microbiology Reporting Templates is collected to reduce the time and similarity for beneficiaries. This is a collection of templates regarding branches of microbiology like bacteriology, mycology, parasitology, virology, and immunology but most commonly bacteriology.

List of Microbiology Reporting Templates

Culture Negative Reports

1. Urine Culture and Sensitivity:  No growth after 24 hours of incubation at 37°C.
2. Pus/ sterile body fluids Culture & Sensitivity: No growth after 48 hours of incubation at 37°C.
3. Blood Culture & Sensitivity: No growth after 5 days of incubation at 37°C.
4. Sputum Culture & Sensitivity: No pathogen isolated after 48 hours of incubation at 37°C.
5. Stool Culture & Sensitivity: No enteric pathogen isolated.

Positive Reports

Urine Culture & Sensitivity

10^5 CFU/mL of E. coli isolated.

10^5 CFU/mL of Coagulase Negative Staphylococci (CoNS) isolated.

Other than Urine specimens Culture and Sensitivity

Light growth of Coagulase Negative Staphylococci (CoNS) isolated.

Heavy growth of Staphylococcus aureus isolated.

Light growth of Staphylococcus aureus isolated.

Moderate growth of Methicillin-resistant Staphylococcus aureus (MRSA) isolated.

Heavy growth of Escherichia coli isolated.

Heavy growth of Klebsiella pneumoniae isolated.

Heavy growth of Enterococcus species isolated.

Heavy growth of Beta (β)- hemolytic streptococci isolated

Heavy growth of Enterococcus species isolated.

Light growth of Pseudomonas aeruginosa isolated.

Heavy growth of Pseudomonas aeruginosa isolated

Moderate growth of Proteus vulgaris isolated.

Moderate growth of Shewanella species isolated.

Microbiology Reporting Templates  for Antimicrobial Sensitivity Testing (AST)

Staphylococcus panel: Ampicillin; Ciprofloxacin; Ofloxacin; Gentamicin; Co-trimoxazole; Chloramphenicol, Clindamycin; Erythromycin; Doxycycline;  Linezolid;  Nitrofurantoin

Enterobacteriaceae panel: Ampicillin, Ciprofloxacin; Ofloxacin; Gentamicin, Co-trimoxazole; Nitrofurantoin; Chloramphenicol, Cefotaxime, Ampicillin/sulbactam

Pseudomonas panel: Ciprofloxacin; Ofloxacin; Gentamicin; Ceftazidime; Meropenem; Imipenem; Tobramycin;  Piperacillin-tazobactam; Amikacin

Acinetobacter Panel: Ciprofloxacin; Co-trimoxazole; Gentamicin; Ceftazidime; Meropenem; Imipenem; Tobramycin; Piperacillin-tazobactam; Amikacin; Doxycycline,Levofloxacin, Tobramycin, Amikacin,Colistin (MIC), Polymyxin B (MIC), Piperacillin,Cefepime, Cefotaxime, Ceftriaxone, and Ampicillin-sulbactam,

Extrinsic Resistance: Amoxicillin-clavulanate, Ampicillin, Amoxicillin, Azithromycin, Aztreonam, Fosfomycin, Cephalosporin I (Cephalothin, cefazolin), Cephalosporin II (Cefuroxime), Chloramphenicol, Clarithromycin, Ertapenem, Erythromycin, Trimethoprim, Cephamycins (cefoxitin, cefotetan),

Enterococcus pannel- Penicillin, Ampicillin, Ciprofloxacin, Norfloxacin, Levofloxacin, Chloramphenicol (except urine), Nitrofurantoin, Erythromycin, Doxycycline, Vancomycin, Teicoplanin, Linezolid, and Gentamicin (HLG,120 µg).

Pneumococcus/Streptococcus pneumoniae Panel: Oxacillin (screening drug, do not report), 0floxacin, Levofloxacin, Chloramphenicol, Erythromycin, Azithromycin, Doxycycline, Vancomycin, Clindamycin, Co-trimoxazole, and Linezolid.

Streptococcus species- viridans group-Penicillin (MIC), Ampicillin, Cefotaxime, Ceftriaxone, Vancomycin, Azithromycin, Erythromycin, Tetracycline, Levofloxacin, 0floxacin, Chloramphenicol, Clindamycin, Linezolid, and Meropenem (MIC).

Note: Please correlate clinically. It is preferred mainly in rare pathogens and normal flora involving as a pathogen in immunocompromized host/ patient.

Contamination, please repeat the mid-stream urine (MSU) sample.

Sputum for Gram Stain

Pus cells<25/LPF

Epithelial cells>10/LPF

Organism: Normal Upper Respiratory Tract Flora Seen

Microbiology Reporting Templates  for Mycology

• Sputum for KOH Mount: Fungal elements not seen
• Sputum for KOH Mount: Yeast cells, yeast cells with budding, and Pseudohyphae seen
• Candida albicans isolated
• Candida species other than albicans isolated.

Keynotes

• Please correlate clinically and if possible resend the specimen for clarification on whether the organism is really a clinical concern or contaminated.
• Urine culture is only a quantitative (semi) method and resting all specimens are a qualitative method of culture.
• Contamination, please repeat mid-stream urine (MSU) sample is requested when mixed growth of microbes on the culture plate of urine inoculated medium from the first urine sample of the patient.
• Qualitative method culture is divided into three groups light, moderate and heavy growth of organisms.
• The most commonly used antimicrobial disks are-Penicillin, Ampicillin, Ciprofloxacin, Ofloxacin, Cotrimoxazole, Gentamycin, Chloramphenicol, Nitrofurantoin, Cefoxitin, Teicoplanin, Erythromycin, Doxycycline, Levofloxacin, Clindamycin, Linezolid, Vancomycin (disk and E-strip), Ceftriaxone, Cefotaxime, Cefixime, Azithromycin, Tetracycline, Nalidixic acid, Meropenem, Amikacin, Aztreonam, Ampicillin sulbactam, Ampicillin clanuvic acid, Penicillin tazobactam, Ceftazidime, Cefepime, Imipenem, Tobramycin, Colistin (E-strip), Polymyxin B (E-strip), Colistin E strip and Oxacillin.