Serratia marcescens colony morphology on nutrient agar, biochemical reactions and antibiogram
Table of Contents
Serratia is a member of the family, Enterobacteriaceae and it is a Gram-negative, facultatively anaerobic, rod-shaped lacking spore and capsule bacterium. This is a Gram-negative rod/ bacilli that thrive in moist environments. It frequently contaminates solutions and hospital equipment and the human reservoirs are the urinary and respiratory tracts as well as the gastrointestinal tract of children. The genus is named after Serafino Serrati, an Italian physicist. In 1819, Bartolomeo Bizio, a pharmacist from Padua, Italy, discovered and named Serratia marcescens when he identified the bacterium as the cause of a miraculous bloody discoloration in a cornmeal mush called polenta. Bizio named Serratia in honor of an Italian physicist named Serrati, who invented the steamboat, and Bizio chose marcescens (from the Latin word for decaying) because the bloody pigment was found to deteriorate quickly.
| Basic Features | Properties |
| 1. Gram Staining | Gram-Negative Rods (GNRs) |
| 2. Spore | Non-Sporing |
| 3. Capsule | Non-Capsulated |
| 4. Motility | Motile |
| 5. Pigment | Positive (Prodigiosin) |
| 6. Growth in potassium cyanide (KCN) medium | Positive |
| 7. Catalase test | Positive |
| 8. Oxidase test | Negative |
| 9. Nitrate reduction test | Positive |
| 10. MR (Methyl Red) test | Negative |
| 11. VP (Voges- Proskauer) assay | Positive |
| 12. OF (Oxidative-Fermentative) test | Fermentative\facultative anaerobe |
| 13. Gas formation | Variable |
| 14. H2S production | Negative |
| 15. Indole formation | Negative |
| 16. Urease/urea hydrolysis test | Positive |
| 17. Citrate/citrate utilization | Positive |
| 18. DNase test | Positive |
| 19. Glucose fermentation | Positive |
| 20. Maltose fermentation | Positive |
| 21. Lactose fermentation | Negative |
| 22. Sucrose fermentation | Positive |
| 23. Xylose fermentation | Negative |
| 24. Mannitol fermentation | Positive |
| 25. Acetate Utilization | Variable |
| 26. ONPG (β-galactosidase) | Positive |
| 27. Phenylalanine Deaminase (PDA)/PPA Test | Negative |
| 28. Lipase test | Positive |
| 29. Esculin Hydrolysis test | Positive |
| 30. Lysine Decarboxylase Test | Positive |
| 31. Ornithine Decarboxylase Test | Positive |
| 32. Arginine Dihydrolase Test | Negative |
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