Enterococcus faecalis in CLED medium, blood agar, Gram staining, MacConkey medium without bile salt and cystal violet and bile esculin test
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Enterococcus faecalis (previously identified as Streptococcus faecalis) is a Gram-positive coccus in singles, pairs, and even short chains which is a commensal member of the gut microbiota of a wide range of microbes. With the advent of antibiotic therapy, it has emerged as a multidrug-resistant (MDR) strain, hospital-acquired, and opportunistic pathogen. Highly virulent strains of this bacterium express a pore-forming exotoxin, called cytolysin, which lyses both bacterial and eukaryotic cells in response to quorum signals.
Gram positive cocci in pairs and short chains of Enterococcus in Gram staining of culture.
This is MacConkey medium lacking bile salt and crystal violet and thus the Enterococcus grew on this medium as shown below.
E. faecalis colony on 5% sheep blood agar is non-hemolytic, circular, and convex colonies with entire margin.
Enterococcus species are capable of growing in the presence of 4% bile and hydrolyzing esculin.
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