Klebsiella oxytoca biochemical tests in TSI, SIM, Urea and Citrate Agar Demonstration
The genus Klebsiella is from the surname of German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacterium with a prominent polysaccharide-based capsule and having a size of 0.5 to 0.8 µm wide to 1-2 µm. Klebsiella oxytoca is naturally found in the intestinal tract, mouth, and nose. This organism is a gut bacterium inside our intestines whereas, outside the gut, it can cause serious infections.
It is commonly spread in healthcare environments like nursing homes and intensive care units (ICU) of the hospital. It is an opportunistic organism and it may cause infections in immunocompromised hosts. K. oxytoca is the etiological agent of pneumonia, urinary tract infections (UTIs), and wound infections. The risk factors for developing infections are prolonged hospital stay, long-term antibiotics use, use of a ventilator, IV catheter, and having diabetes.
Table of Contents
| Basic Features | Properties |
| 1. Gram Staining | Gram-Negative Rods (GNRs) |
| 2. Spore | Non-Sporing |
| 3. Capsule | Positive |
| 4. Motility | Negative (Non-motile) |
| 5. Pigment | Negative |
| 6. Growth in potassium cyanide (KCN) medium | Positive |
| 7. Catalase test | Positive |
| 8. Oxidase test | Negative |
| 9. Nitrate reduction test | Positive |
| 10. MR (Methyl Red) test | Negative |
| 11. VP (Voges- Proskauer) assay | Positive |
| 12. OF (Oxidative-Fermentative) test | Fermentative |
| 13. Gas | Variable |
| 14. H2S production | Negative |
| 15. Indole formation | Positive |
| 16. Urease/urea hydrolysis test | Positive |
| 17. Citrate/ citrate utilization | Positive |
| 18. DNase test | Negative |
| 19. Glucose fermentation | Positive |
| 20. Maltose fermentation | Positive |
| 21. Lactose fermentation | Positive |
| 22. Sucrose fermentation | Positive |
| 23. Xylose fermentation | Positive |
| 24. Mannitol fermentation | Positive |
| 25. Acetate Utilization | Variable |
| 26. ONPG (β-galactosidase) | Positive |
| 27. Phenylalanine Deaminase (PDA)/PPA Test | Positive |
| 28. Lipase test | Negative |
| 29. Esculin Hydrolysis test | Positive |
| 30. Lysine Decarboxylase Test | Positive |
| 31. Ornithine Decarboxylase Test | Negative |
| 32. Arginine Dihydrolase Test | Negative |
In the world of modern diagnostics, speed, accuracy, and comprehensiveness are critical—especially when dealing with…
Introduction In today’s rapidly evolving healthcare landscape, the demand for fast, accurate, and accessible diagnostic…
Introduction to MYCOscreen18 PCR Panel Identifying fungal infections quickly and accurately is a critical challenge…
The increasing burden of infectious diseases demands diagnostic solutions that are not only rapid but…
Fungal infections represent a growing global health challenge, causing over 1.7 million deaths annually, with…
Introduction Klebsiella aerogenes (formerly Enterobacter aerogenes) is an opportunistic Gram-negative, facultative anaerobic bacterium responsible for serious healthcare-associated infections…
View Comments
Just desire to say your article is as surprising. The clearness in your post is simply great and i can assume you are an expert on this subject. Well with your permission let me to grab your RSS feed to keep updated with forthcoming post. Thanks a million and please keep up the enjoyable work.
What i do not realize is actually how you're not really much more well-liked than you may be right now. You're so intelligent. You realize therefore considerably relating to this subject, made me personally consider it from so many varied angles. Its like women and men aren't fascinated unless it’s one thing to accomplish with Lady gaga! Your own stuffs outstanding. Always maintain it up!
Just wish to say your article is as astounding. The clarity in your post is just cool and i could assume you're an expert on this subject. Well with your permission allow me to grab your feed to keep up to date with forthcoming post. Thanks a million and please keep up the rewarding work.
What i do not understood is actually how you're no longer really a lot more well-favored than you may be now. You are very intelligent. You know therefore considerably in the case of this topic, produced me for my part imagine it from a lot of various angles. Its like men and women don't seem to be fascinated until it is one thing to accomplish with Woman gaga! Your individual stuffs nice. All the time deal with it up!
Lovely just what I was looking for.Thanks to the author for taking his clock time on this one.
Its like you read my mind! You seem to know a lot about this, like you wrote the book in it or something. I think that you could do with a few pics to drive the message home a bit, but other than that, this is excellent blog. An excellent read. I will certainly be back.
I like what you guys are up also. Such intelligent work and reporting! Carry on the excellent works guys I?¦ve incorporated you guys to my blogroll. I think it'll improve the value of my website :)
I would like to thank you for the efforts you have put in writing this web site. I'm hoping the same high-grade web site post from you in the upcoming also. In fact your creative writing skills has inspired me to get my own site now. Really the blogging is spreading its wings fast. Your write up is a great example of it.
I believe this web site has very wonderful written content content.