All Notes

Klebsiella pneumoniae: Introduction, Identification Features, Keynotes, and Klebsiella pneumoniae Footages

Klebsiella pneumoniae biochemical tests in TSI, SIM, Citrate and Urea agar

Introduction

The genus Klebsiella is from the surname of  German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacterium with a prominent polysaccharide-based capsule and having a size of 0.5 to 0.8 µm wide to 1-2 µm. It is found everywhere in nature and also in bacterial flora in our intestines. It may cause severe bronchopneumonia, urinary tract infections, nosocomial infections, wound infections, septicemia, meningitis, and rarely diarrhea too. Subspecies of Klebsiella pneumoniae are Klebsiella pneumoniae subspecies ozaenae, Klebsiella pneumoniae subspecies pneumoniae, and Klebsiella pneumoniae subspecies rhinoscleromatis.

Fig. Klebsiella pneumoniae Mucoid Lactose Fermenting (MLF) Colonies on CLED Medium of Urine Culture

Identification Features of Klebsiella pneumoniae

Basic FeaturesProperties
1. Gram StainingGram-Negative Rods (GNRs)
2. SporeNon-Sporing
3. CapsulePositive
4. MotilityNegative (Non-motile)
5. PigmentNegative
6. Growth in potassium cyanide (KCN) mediumPositive
7. Catalase testPositive
8. Oxidase testNegative
9. Nitrate reduction testPositive
10. MR (Methyl Red) testNegative
11. VP (Voges- Proskauer) assayPositive
12. OF (Oxidative-Fermentative) testFermentative
13. GasPositive
14. H2S productionNegative
15. Indole formationNegative
16. Urease/ urea hydrolysis testPositive
17. Citrate/ citrate utilizationPositive
18. DNase testNegative
19. Glucose fermentationPositive
20. Maltose fermentationPositive
21. Lactose fermentationPositive
22. Sucrose fermentationPositive
23. Xylose fermentationPositive
24. Mannitol fermentationPositive
25. Acetate UtilizationVariable
26. ONPG (β-galactosidase)Positive
27. Phenylalanine Deaminase (PDA)/PPA TestNegative
28. Lipase testNegative
29. Esculin Hydrolysis testPositive
30. Lysine Decarboxylase TestPositive
31. Ornithine Decarboxylase TestNegative
32. Arginine Dihydrolase TestNegative
Table: Properties of Klebsiella pneumoniae

Klebsiella Footages

K. pneumoniae of blood sample growth in BHI broth

Fig. K. pneumoniae of blood sample growth in BHI broth

K. pneumoniae colony characteristics on nutrient agar

Fig. K. pneumoniae colony characteristics on nutrient agar

K. pneumoniae colony morphology on MacConkey agar

Fig. K. pneumoniae colony morphology on MacConkey agar

K. pneumoniae biochemical tests in TSI, SIM, Citrate and Urea agar

Fig. K. pneumoniae biochemical tests in TSI, SIM, Citrate and Urea agar

Mucoid lactose fermenting colony of K. pneumoniae on MacConkey agar

Fig. Mucoid lactose fermenting colony of K. pneumoniae on MacConkey agar

Antimicrobial Susceptibility Testing (AST) Pattern of K. pneumoniae showing MDR

Fig. Antimicrobial Susceptibility Testing (AST) Pattern of K. pneumoniae showing MDR

Turbid urine of UTI patient having numerous pus cells and non- motile bacteria

Fig. Turbid urine of UTI patient having numerous pus cells and non-motile bacteria

Pus cells and bacteria in urine microscopy at a high magnification

Fig. Pus cells and bacteria in urine microscopy at high magnification 1600X

K. pneumoniae growth on blood agar

Fig. K. pneumoniae growth on blood agar

Klebsiella pneumoniae growth on MacConkey medium

Fig. K. pneumoniae growth on MacConkey medium

Gram-negative bacilli (GNB) of Klebsiella pneumoniae in Gram staining of culture

Fig. Gram-negative bacilli (GNB) of K. pneumoniae in Gram staining of culture

Klebsiella pneumoniae biochemical reactions in MIU, TSI and citrate agar

Fig. K. pneumoniae biochemical reactions in MIU, TSI, and citrate agar

Antibiogram of K. pneumoniae isolated from urine specimen

Fig. Antibiogram of K. pneumoniae isolated from urine specimen

K. pneumoniae in polychrome Loeffler methylene blue wet mount microscopy at high magnification

Fig. K. pneumoniae in polychrome Loeffler methylene blue wet mount microscopy at high magnification (1200X)

K. pneumoniae capsule in negative staining

Fig. K. pneumoniae capsule (clear zone around the bacterium) in negative staining at a magnification of 2000X

Further Reading

  1. https://www.sciencedirect.com/topics/medicine-and-dentistry/klebsiella-pneumoniae
  2. https://universe84a.com/klebsiella-pneumoniae/
  3. Cowan & Steel’s Manual for identification of Medical Bacteria. Editors: G.I. Barron & R.K. Felthani, 3rd ed 1993, Publisher Cambridge University Press.
  4. Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
  5. Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
  6. Textbook of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.
Medical Lab Notes

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Tags: Antibiogram of K. pneumoniae isolated from urine specimenAntibiogram of Klebsiella pneumoniae isolated from urine specimenAntimicrobial Susceptibility Testing (AST) Pattern of K. pneumoniae showing MDRAntimicrobial Susceptibility Testing (AST) Pattern of Klebsiella pneumoniae showing MDRBacteriaCitrateCitrate and Urea agarGNBGNRGram-negative bacilli (GNB) of Klebsiella pneumoniae in Gram staining of cultureIdentification Features of Klebsiella pneumoniaeIntroduction of Klebsiella pneumoniaeK. pneumoniae biochemical tests in TSIK. pneumoniae capsule in negative stainingK. pneumoniae colony characteristics on nutrient agarK. pneumoniae colony morphology on MacConkey agarK. pneumoniae in polychrome Loeffler methylene blue wet mount microscopy at high magnificationKlebsiellaKlebsiella pneumoniaeKlebsiella pneumoniae biochemical reactions in MIUKlebsiella pneumoniae capsule in negative stainingKlebsiella pneumoniae colony characteristics on nutrient agarKlebsiella pneumoniae colony morphology on MacConkey agar Klebsiella pneumoniae biochemical tests in TSIKlebsiella pneumoniae FootagesKlebsiella pneumoniae growth on blood agarKlebsiella pneumoniae growth on MacConkey mediumKlebsiella pneumoniae in polychrome Loeffler methylene blue wet mount microscopy at high magnificationKlebsiella pneumoniae of blood sample growth in BHI brothLFMacConkey agarMedicallabnotesMedlabsolutions9MicrohubMLFMucoid lactose fermenting colony of K. pneumoniae on MacConkey agarMucoid lactose fermenting colony of Klebsiella pneumoniae on MacConkey agarPus cells and bacteria in urine microscopy at a high magnificationPus cells and bacteria in urine microscopy at high magnification 1600XSIMTSI and citrate agarTurbid urine of UTI patient having numerous pus cells and non- motile bacteriaTurbid urine of UTI patient having numerous pus cells and non-motile bacteriaUniverse84aUrea agar
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