Molds-Disk Diffusion Assay: Introduction, Principle, Methods, Clinical Significance, and Keynotes

Introduction The disk diffusion assay for molds is a standardized antifungal susceptibility testing (AFST) method that evaluates the sensitivity or resistance of filamentous fungi to antifungal agents. While primarily standardized for yeasts (e.g., CLSI M44-A2), disk diffusion has also been adapted for molds, particularly Aspergillus …

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