Rose Bengal Agar (RBA)- Introduction, Principle, Composition, Test Procedure, Colony Characteristics, Uses, and Keynotes

Fungal growth on Rose Bengal Agar (RBA) at centre

Introduction Rose Bengal Agar (RBA) is a selective medium for isolating fungi. It contains rose bengal dye, which inhibits bacterial growth. This medium supports the growth of various fungi. Chloramphenicol in RBA further suppresses bacterial contamination. Scientists and clinicians use it for environmental and clinical …

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Clinical Laboratory Gallery: Introduction, Contents, and Brief Description of Photos

Wet Mount Microscopy-Ready slide for observation

Introduction ‘Clinical Laboratory Gallery’ is a collection of genuine photos regarding stream of Clinical Laboratory like Stool and Urine Section (SUS), Phlebotomy, Clinical Haematology, Clinical Biochemistry, Blood Banking and Transfusion medicine, Microbiology and Immunology, Cytology and Histopathology, and Molecular Biology. Contents Collection of images are …

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XLD Agar: Introduction, Composition, Principle, Preparation, Test Requirements, Procedure, Colony Characteristics, Modifications, Uses, and Limitations

Salmonella Typhi (black colony) and Shigella growth on xylose lysine deoxycholate (XLD) agar

Introduction of XLD Agar XLD agar is a selective, differential, and indicator medium for the isolation of enteric pathogens. XLD stands for xylose lysine deoxycholate. It also supports the growth of more fastidious enteric organisms. XLD agar was developed by Taylor. It was specially designed to allow the …

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Mannitol Salt Agar (MSA): Introduction, Composition, Principle, Test Requirement, Procedure, Result -Interpretation, Limitation, and Keynotes

Staphylococcus aureus , CoNS and inhibited growth of Escherichia coli on MSA

Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify S. aureus from clinical and non-clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective is due to very high salt (7.5%) compared with other media. Gram-Positive Staphylococcus: …

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TCBS Agar : Introduction, Composition,Principle, Test Requirement,Procedure, Result -Interpretation, Limitation, and Keynotes

Vibrio cholerae colony morphology on TCBS agar

Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and Thiosulfate Citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar …

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Czapek Dox Agar: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

Aspergillus flavus growth on Czapek Dox Agar

Introduction of Czapek Dox Agar Czapek medium also called Czapek’s agar (CZA) from the surname of inventor Czech botanist Friedrich Johann Franz Czapek (1902-1903). Later CZA was modified by another American inventor, chemist Arthur Wayland Dox  (1910) and thus its name became’ Czapek-Dox medium’ and formula prepared …

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Dermatophyte Test Medium (DTM): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications,Uses, and Keynotes

Dermatophytes growth on Dermatophyte Test Medium (DTM)

Introduction of Dermatophyte Test Medium (DTM) Dermatophyte Test Medium (DTM) is a selective and differential medium recommended for the cultivation and isolation of pathogenic dermatophytes. The Dermatophytes are a distinct group of fungi that infect the hair, skin, and nails in humans producing a variety of …

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Potato Dextrose Agar (PDA): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

A. fumigatus growth on PDA

Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA) is a fungal medium and its ingredients are clear from its name it contains potato ( vegetable), dextrose ( sugar), and agar ( solidifying agent). PDA  is recommended for the isolation and enumeration of fungi …

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