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Introduction TB-LAMP (Tuberculosis Loop-Mediated Isothermal Amplification) is a manual, rapid molecular diagnostic test endorsed by the World Health Organization (WHO) to detect the Mycobacterium tuberculosis complex (MTBC) in sputum samples. It serves as a highly sensitive alternative to traditional sputum smear microscopy, particularly optimized for …
Introduction The NALC-NaOH (N-acetyl-L-cysteine–sodium hydroxide) method is the gold standard for processing clinical specimens in mycobacteriology laboratories. It prepares contaminated samples (like sputum) for successful culture on liquid media (such as the BD BACTEC™ MGIT™ 960) and solid media. Clinical samples from non-sterile sites naturally …
Introduction The BD BACTEC™ MGIT™ 960 (Mycobacteria Growth Indicator Tube) is a fully automated, high-volume culture system designed for the rapid detection of mycobacteria and subsequent drug susceptibility testing (DST). Developed by Becton Dickinson (BD), this nonradiometric system addresses the critical clinical need for accelerated …
Introduction to Bacterial Diseases Bacterial diseases are illnesses caused by pathogenic bacteria entering the body, multiplying, and releasing toxins that damage host tissues. These pathogens spread through contaminated food, water, airborne droplets, sexual contact, or vectors. Despite the advent of antibiotics, bacterial infections remain a …
Introduction The Mycobacteria Growth Indicator Tube (MGIT) system is a high-throughput, automated method optimized for the rapid isolation and detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria (NTM). [Initial Stage] Oxygen is high —–> Quenches sensor —–> NO FLUORESCENCE [Bacterial Growth] Oxygen consumed —-> Frees …
Encircle the correct Answer of the Following question. 1) What is the full form of LQAS? 2) What are the components of the Quality Assurance system? 3) LQAS is based on… 4) GeneXpert testing detects… 5) The slide positivity rate is determined in LQAS is …
Introduction First-Line Drug Susceptibility Testing (FL-DST or sometimes referred to in context as SL-DST when covering all first-line agents: Rifampicin, Isoniazid, Ethambutol, Pyrazinamide) is a crucial laboratory procedure used to determine if a patient’s Mycobacterium tuberculosis (Mtb) strain is sensitive or resistant to primary anti-TB …
Introduction Second-line drug susceptibility testing (SL-DST) for tuberculosis is a crucial laboratory procedure designed to detect resistance to anti-TB drugs used when first-line treatment fails (i.e., multidrug-resistant TB – MDR-TB). Principle SL-DST measures the susceptibility of Mycobacterium tuberculosis to specific agents by determining the Critical …