Pleural Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Pleural fluid for laboratory diagnosis

Introduction Pleural fluid culture and sensitivity is a diagnostic test used to identify and determine the antibiotic susceptibility of microorganisms present in the pleural fluid. The pleural fluid is a clear fluid that accumulates between the two layers of the pleura, the membrane that surrounds …

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TB PCR for Sputum: Introduction, Test Result, Unit, Normal Range, Assaying Method, Clinical Significance, and Keynotes

TB PCR for Sputum-Mycobacterium tuberculosis Detected but Rifampicin Resistance not Detection

Introduction TB PCR (polymerase chain reaction) for sputum is a laboratory test used in the diagnosis of tuberculosis (TB). It is a molecular diagnostic technique that detects the presence of Mycobacterium tuberculosis (the bacterium that causes TB) DNA in sputum samples. Traditional methods of diagnosing …

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GeneXpert Test For Tuberculosis: Introduction, Principle, Test Requirements, Test Procedure, Result -Interpretation, Advantages, Disadvantage, and Keynotes

GeneXpert Test For Tuberculosis-Mycobacterium tuberculosis (MTB) Detected High

Introduction of GeneXpert Test GeneXpert test is a molecular test for tuberculosis diagnosis by detecting the presence of  Mycobacterium tuberculosis (bacteria), as well as testing for resistance to the drug rifampicin. Principle of GeneXpert Test GeneXpert test is a highly sensitive and specific test for tuberculosis(TB) diagnosis that detects DNA sequences …

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Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …

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QuantiFERON-TB Gold (QFT) and Tuberculin Skin Test (TST): Introduction, Differences, and Keynotes

QuantiFERON-TB Gold (QFT) and Tuberculin Skin Test (TST): Introduction, Differences, and Keynotes

Introduction of QFT) and TST Interferon-Gamma Release Assays (IGRAs) are a newer blood test for tuberculosis that detects TB infection by measuring the release of interferon-gamma (IFN-g) from patient T cells after stimulation of a whole blood sample with highly specific TB antigens. QuantiFERON-TB Gold …

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Bacteria and Virus: Introduction, Differences, and Keynotes

Staphylococcus aureus growth on blood agar of clinical sample, pus

Introduction of Bacteria and Virus Bacteria are member of a large group of living unicellular microbes having size of cocci 0.5-1.0 µm while bacilli 1-10 µm×3-10µm which have cell walls but lacking an organized nucleus, including some that can cause disease like pneumonia, tuberculosis, food …

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