Methyl Red Test: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Positive and Negative Bacteria, and Limitations

Methyl Red Test-Positive and Negative Test Results

Introduction of Methyl Red Test The Methyl Red test is for enteric gram-negative rods, as part of identification to species level. Its short name is MR test. Principle of Methyl Red Test The methyl red (MR) test uses to determine if an organism is able …

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Czapek Dox Agar: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

Aspergillus flavus growth on Czapek Dox Agar

Introduction of Czapek Dox Agar Czapek medium also called Czapek’s agar (CZA) from the surname of inventor Czech botanist Friedrich Johann Franz Czapek (1902-1903). Later CZA was modified by another American inventor, chemist Arthur Wayland Dox  (1910) and thus its name became’ Czapek-Dox medium’ and formula prepared …

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Dermatophyte Test Medium (DTM): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications,Uses, and Keynotes

Dermatophytes growth on Dermatophyte Test Medium (DTM)

Introduction of Dermatophyte Test Medium (DTM) Dermatophyte Test Medium (DTM) is a selective and differential medium recommended for the cultivation and isolation of pathogenic dermatophytes. The Dermatophytes are a distinct group of fungi that infect the hair, skin, and nails in humans producing a variety of …

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L-pyrrolidinyl-β-naphthylamide (PYR) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretations, and Limitations

L-pyrrolidinyl-β-naphthylamide (PYR) Test-Positive

Introduction of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) test uses to identify the capability of organisms utilizing substrate, PYR by L-pyrroglutamyl amino-peptidase. Application of this test are as follows: Principle of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) acts as a substrate for the detection of the enzyme …

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Bile Solubility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Bile Solubility Test- Positive and Negative

Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …

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Optochin Susceptibility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Optochin Susceptibility Test-Positive

Optochin Susceptibility Test Optochin Susceptibility Test uses for presumptive identification of Streptococcus pneumoniae from viridans streptococci.  Principle of Optochin Susceptibility Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low concentration (5ug/ml). Optochin may also inhibit other viridians streptococci, but only …

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Candida Mannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes

Candida Mannan ELISA Detection Kit: Introduction, Principle of ELISA, Product Characteristics, and Keynotes

Introduction of Candida Mannan ELISA Detection Kit Candida Mannan ELISA Detection is used for the specific detection of Invasive Candidiasis and Candidemia. It works on the principle of competitive Enzyme-linked Immunosorbent Assay (ELISA). It is applied for the quantitative detection of Candida mannan antigen in …

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ASO (Anti-streptococcal O)Test: Introduction, Principle, Procedure, Normal Range, Result- Interpretation, and Keynotes

ASO Test Result

Introduction of ASO Test ASO stands for Antistreptococcal O and it is an antibody targeted against streptolysin O, a toxic enzyme produced by group A Streptococcus bacterium. Antistreptococcal O and anti -DNase are the most common of several antibodies that are produced by the body’s immune system in response to a strep infection …

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