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Introduction Streptococcus pneumoniae, commonly known as pneumococcus, is a bacterium that can cause a wide range of infections in humans. It is one of the most significant pathogens responsible for various diseases, particularly pneumonia, meningitis, and otitis media (middle ear infections). Characteristics: Transmission: Diseases caused …
Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …
Optochin Susceptibility Test Optochin Susceptibility Test uses for presumptive identification of Streptococcus pneumoniae from viridans streptococci. Principle of Optochin Susceptibility Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low concentration (5ug/ml). Optochin may also inhibit other viridians streptococci, but only …
Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …
Introduction of Gram Stain Gram stain is the most commonly used routine stain in microbiology for the identification of microbes and also to know the evidence of etiological agents in clinical specimens. Gram staining is a differential stain and thus it applies to differentiate Gram-positive …