Coomb Test: Introduction, Types, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Clinical Significance

Coomb Test Reagents-Anti- D serum and Anti-human globulin (AHG) or Coomb’s reagent

Introduction of Coomb Test Direct Coomb Test (DCT) is a type of Coomb test also called the anti-globulin test. It is of two types-Direct Coomb’s test (DCT) and the Indirect Coomb’s test (ICT). DCT uses to detect sensitized red blood cells (RBCs) while ICT detects …

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Indirect Coomb Test (ICT): Introduction, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Clinical Significance

Indirect Coomb Test Observation

Introduction of  Indirect Coomb Test (ICT) The indirect Coomb Test is a type of Coomb’s test also called the anti-globulin test. It is of two types-Direct Coomb’s test (DCT) and the Indirect Coomb’s test (ICT). DCT uses to detect sensitized red blood cells (RBCs) while …

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Direct Coomb Test (DCT): Introduction, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Clinical Significance

Direct Coomb Test (DCT) Observation

Introduction of Direct Coomb Test (DCT) Direct Coomb Test (DCT) is a type of Coomb test also called the anti-globulin test. It is of two types-Direct Coomb’s test (DCT) and the Indirect Coomb’s test (ICT). DCT uses to detect sensitized red blood cells (RBCs) while …

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Indoxyl Acetate Disk Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Indoxyl Acetate Disk Test-Positive and Negative Results

Introduction of Indoxyl Acetate Disk Test  Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …

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Culture Media: Introduction, Common Ingredients, Types, Preparation, and Use of Common Culture Medium, Keynotes, and Related Images

Culture media with variety of microbes-Micrococcus growth on nutrient agar (upper left), Lactose fermenting(Lf) and non-lactose fermenting (NLF) colonies of Gram negative bacteria colonies on MacConkey medium, Hameophilus influenzae colony morphology on chocolate agar(lower left), beta-hemolytic colonies of Staphylococcus aureus( middle), and Pseudomonas aeruginosa antibiogram pattern on Muller-Hinton agar(MHA)

Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …

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Anaerobic Bacteria Culture: Introduction, Principle, Procedure, and Result Interpretation

Anaerobic Bacteria Culture: Introduction, Principle, Procedure, and Result Interpretation

Introduction of Anaerobic Bacteria Culture Obligate anaerobes can not grow in the presence of oxygen i.e. air. These organisms (obligate anaerobic bacteria) die rapidly on exposure to air, therefore for maintaining anaerobiosis various methods have been devised for anaerobic culture. Principle for Anaerobic Bacteria Culture/Cultivation …

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