Haemophilus

Haemophilus Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Limitations, and Keynotes

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Use of bacitracin 10 U on chocolate agar for screening Haemophilus of sputum culture

Introduction of Haemophilus Serotyping Haemophilus Serotyping helps to differentiate serotypes of Haemophilus influenzae. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, encapsulated strains of Haemophilus influenzae may …

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Culture Media: Introduction, Common Ingredients, Types, Preparation, and Use of Common Culture Medium, Keynotes, and Related Images

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Culture media with variety of microbes-Micrococcus growth on nutrient agar (upper left), Lactose fermenting(Lf) and non-lactose fermenting (NLF) colonies of Gram negative bacteria colonies on MacConkey medium, Hameophilus influenzae colony morphology on chocolate agar(lower left), beta-hemolytic colonies of Staphylococcus aureus( middle), and Pseudomonas aeruginosa antibiogram pattern on Muller-Hinton agar(MHA)

Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …

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Some Typical Arrangements of Bacteria: List, Keynotes, and, Few Related Images

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Some Typical Shape, Size and Arrangements of Bacteria

List of Some Typical Arrangements of Bacteria In this chapter, we discuss some typical arrangements of bacteria like Staphylococcus, Streptococcus, pneumococcus, Enterococcus, Micrococcus, Neisseria species, Bacillus anthracis, Diphtheroids, Corynebacterium diphtheriae, Listeria monocytogenes, Vibrio cholerae, Campylobacter, Helicobacter, Treponema, Leptospira, Borrelia, etc. S.No. Arrangements  Organisms 1. Gram-positive cocci …

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India Ink Preparation: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Uses, Keynotes, and Negative Staining Footages

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India Ink Preparation: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Uses and Keynotes

Introduction of India Ink Preparation Cryptococcal meningitis occurs in immunodeficient patients and when meningitis is clinically suspected, for example, patients with HIV/AIDS, or when yeast cells with lymphocytes are detected when performing Cerebrospinal fluid (CSF) cell count or examining Gram smear, examine India ink preparation …

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Use of X, V, and XV Growth Factors Disks for Haemophilus species Identification: Introduction, Species Identification, Keynotes, and Haemophilus Footages

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Use of X, V, and XV Growth Factors Disks for Haemophilus species Identification: Introduction, Species Identification, Keynotes, and Haemophilus Footagaes

Introduction of Haemophilus The genus Haemophilus is a pleomorphic Gram-negative coccobacilli fastidious bacteria and thus they do not grow on an ordinary medium like nutrient agar and they need chocolate agar to grow (H. influenzae) due to having X, V, and both X and V growth factors in it. …

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Chocolate Agar: Introduction, Composition, Principle, Preparation Requirements, Testing Procedure, Colony Characteristics, Uses, Keynotes, and Chocolate Agar Footages

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Chocolate Agar: Introduction,Composition, Principle, Preparation Requirements,Testing Procedure, Colony Characteristics, Uses, Keynotes and Chocolate Agar Footages

Introduction of Chocolate agar  Chocolate Agar short form is (CHOC). It is a non-selective, enriched growth medium that is the lysed blood agar. The agar is named for its color when the red blood cells (RBCs) lysis gives the medium a chocolate-brown color without having chocolate …

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Satellitism Test for the Identification of Haemophilus influenzae: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

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Satellitism Test for the Identification of Haemophilus influenzae- Introduction, Principle, Test Requirements, Procedure, Result -Interpretation and Keynotes

Introduction Satellitism test or satellite test is applied to identify Haemophilus influenzae. It is a fastidious bacterium and thus it needs extra ingredients like hemin (factor X) and  NAD (factor V) to grow. It uses the X factor to produce essential respiratory enzymes such as cytochromes, catalases, …

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