Test procedure (specimen/organism inoculation) Archives

XLD Agar: Introduction, Composition, Principle, Preparation, Test Requirements, Procedure, Colony Characteristics, Modifications, Uses, and Limitations

February 4, 2023January 25, 2023 by Medical Lab Notes

Salmonella Typhi (black colony) and Shigella growth on xylose lysine deoxycholate (XLD) agar

Introduction of XLD Agar XLD agar is a selective, differential, and indicator medium for the isolation of enteric pathogens. XLD stands for xylose lysine deoxycholate. It also supports the growth of more fastidious enteric organisms. XLD agar was developed by Taylor. It was specially designed to allow the …

Mannitol Salt Agar (MSA): Introduction, Composition, Principle, Test Requirement, Procedure, Result -Interpretation, Limitation, and Keynotes

February 17, 2023January 25, 2023 by Medical Lab Notes

Staphylococcus aureus , CoNS and inhibited growth of Escherichia coli on MSA

Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify S. aureus from clinical and non-clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective is due to very high salt (7.5%) compared with other media. Gram-Positive Staphylococcus: …

Potato Dextrose Agar (PDA): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

February 17, 2023January 21, 2023 by Medical Lab Notes

Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA) is a fungal medium and its ingredients are clear from its name it contains potato ( vegetable), dextrose ( sugar), and agar ( solidifying agent). PDA is recommended for the isolation and enumeration of fungi …

Culture Media: Introduction, Common Ingredients, Types, Preparation, and Use of Common Culture Medium, Keynotes, and Related Images

January 13, 2023November 23, 2022 by Medical Lab Notes

Culture media with variety of microbes-Micrococcus growth on nutrient agar (upper left), Lactose fermenting(Lf) and non-lactose fermenting (NLF) colonies of Gram negative bacteria colonies on MacConkey medium, Hameophilus influenzae colony morphology on chocolate agar(lower left), beta-hemolytic colonies of Staphylococcus aureus( middle), and Pseudomonas aeruginosa antibiogram pattern on Muller-Hinton agar(MHA)

Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …

MacConkey Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses, and Keynotes

December 1, 2022May 7, 2022 by Medical Lab Notes

Introduction of MacConkey Agar MacConkey agar (MAC) uses for the culture of gram-negative bacteria and therefore Enterobacteriaceae belonging bacteria grow well on this medium and coliforms also enjoy this medium. MAC is a modification of Neutral Red Bile Salt Agar developed by MacConkey. It was one of the …