Limitation

Mannitol Salt Agar (MSA): Introduction, Composition, Principle, Test Requirement, Procedure, Result -Interpretation, Limitation, and Keynotes

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Staphylococcus aureus , CoNS and inhibited growth of Escherichia coli on MSA

Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify S. aureus from clinical and non-clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective is due to very high salt (7.5%) compared with other media. Gram-Positive Staphylococcus: …

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TCBS Agar : Introduction, Composition,Principle, Test Requirement,Procedure, Result -Interpretation, Limitation, and Keynotes

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Vibrio cholerae colony morphology on TCBS agar

Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and Thiosulfate Citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar …

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Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

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Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …

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