Culture Media: Introduction, Common Ingredients, Types, Preparation, and Use of Common Culture Medium, Keynotes, and Related Images

Culture media with variety of microbes-Micrococcus growth on nutrient agar (upper left), Lactose fermenting(Lf) and non-lactose fermenting (NLF) colonies of Gram negative bacteria colonies on MacConkey medium, Hameophilus influenzae colony morphology on chocolate agar(lower left), beta-hemolytic colonies of Staphylococcus aureus( middle), and Pseudomonas aeruginosa antibiogram pattern on Muller-Hinton agar(MHA)

Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …

Read more

Chocolate Agar: Introduction, Composition, Principle, Preparation Requirements, Testing Procedure, Colony Characteristics, Uses, Keynotes, and Chocolate Agar Footages

Chocolate Agar: Introduction,Composition, Principle, Preparation Requirements,Testing Procedure, Colony Characteristics, Uses, Keynotes and Chocolate Agar Footages

Introduction of Chocolate agar  Chocolate Agar short form is (CHOC). It is a non-selective, enriched growth medium that is the lysed blood agar. The agar is named for its color when the red blood cells (RBCs) lysis gives the medium a chocolate-brown color without having chocolate …

Read more

Satellitism Test for the Identification of Haemophilus influenzae: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

Satellitism Test for the Identification of Haemophilus influenzae- Introduction, Principle, Test Requirements, Procedure, Result -Interpretation and Keynotes

Introduction Satellitism test or satellite test is applied to identify Haemophilus influenzae. It is a fastidious bacterium and thus it needs extra ingredients like hemin (factor X) and  NAD (factor V) to grow. It uses the X factor to produce essential respiratory enzymes such as cytochromes, catalases, …

Read more