Molds-Broth Microdilution Testing (CLSI)-Introduction, Principle, Clinical Significance, and Keynotes

Introduction Invasive mold infections such as those caused by Aspergillus, Fusarium, Scedosporium, and Mucorales present significant clinical challenges, especially in immunocompromised hosts. Mortality rates are high, and empirical antifungal therapy often fails due to intrinsic or acquired resistance. Standardized antifungal susceptibility testing (AFST) is therefore …

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Potato Dextrose Agar (PDA): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

A. fumigatus growth on PDA

Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA) is a fungal medium and its ingredients are clear from its name it contains potato ( vegetable), dextrose ( sugar), and agar ( solidifying agent). PDA  is recommended for the isolation and enumeration of fungi …

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