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CLSI M38

Molds-Broth Microdilution Testing (CLSI)-Introduction, Principle, Clinical Significance, and Keynotes

September 11, 2025 by Medical Lab Notes

Introduction Invasive mold infections such as those caused by Aspergillus, Fusarium, Scedosporium, and Mucorales present significant clinical challenges, especially in immunocompromised hosts. Mortality rates are high, and empirical antifungal therapy often fails due to intrinsic or acquired resistance. Standardized antifungal susceptibility testing (AFST) is therefore …

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Categories All Notes, Basic Microbiology, Miscellaneous, Mycology Tags AFST, Amphotericin B, Antifungal resistance, Antifungal susceptibility testing, Aspergillus, Aspergillus fumigatus ATCC 204305, Azoles, Broth microdilution, Candida parapsilosis ATCC 22019, CLSI M38, Conidial suspension, Echinocandins, Endpoint reading, Epidemiological cutoff values, Filamentous fungi, Fungi, Fungus, Fusarium, Hyphal fragments, Incubation 35°C, inoculum standardization, Invasive mold infections, Medicallabnotes, Medlabsolutions, Medlabsolutions9, MIC determination, Microhub, Microtiter plate, minimum inhibitory concentration, molds, MOPS buffer, mruniversei, Mucorales, quality control strains, reference method, RPMI 1640 medium, Scedosporium, Serial dilutions, Universe84a

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