Pulsed-field gel electrophoresis (PFGE)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Pulsed-field gel electrophoresis (PFGE)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction Pulsed-field gel electrophoresis (PFGE) is a powerful laboratory technique used in molecular biology and genetics to separate and analyze large DNA molecules, such as genomic DNA, by their size. PFGE is particularly valuable when studying organisms with complex genomes, such as bacteria, yeast, and …

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E-Test-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Placing Vancomycin E-Test Strip for Staphylococcus aureus

Introduction The E-Test, short for “Epsilometer Test,” is a diagnostic tool used in clinical microbiology and antimicrobial susceptibility testing. It is a method for determining the minimum inhibitory concentration (MIC) of antibiotics or antimicrobial agents against a particular bacterial or fungal pathogen. The E-Test is …

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Candida Chromagar: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Candida Chromagar: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Introduction Candida Chromagar is a selective and differential agar medium used for the isolation and identification of Candida species. HiMedia is a well-known manufacturer of microbiological culture media and laboratory products. Here are some keywords related to this agar: Principle The principle of Candida Chromagar …

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X-Match: Introduction, Principle, Test-Procedure, Result-Interpretation, Application, and Keynotes

X-Match: Introduction, Principle, Test-Procedure, Result-Interpretation, Application, and Keynotes

Introduction of X-Match The crossmatch is an important step in ensuring compatibility between the donor’s blood and the recipient’s blood before a blood transfusion takes place. It is performed to detect any potential incompatibilities that could lead to adverse reactions in the recipient. During a …

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Fluorometer: Introduction, Principle, Test Requirements, Handling, Result-Interpretation, and Keynotes

Flurometer

Introduction of Fluorometer A fluorometer is an analytical instrument used to measure the fluorescence emitted by a sample. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength. Fluorometers are widely used in fields …

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Leptospira Test-Introduction, Principle, Test Requirement, Procedure, Test Result, Test Method, Result-Interpretation, and Keynotes

Leptospira Test-Rapid Test Device, Diluent, and Specimen

Introduction of Leptospira Rapid Diagnostic Test  Leptospira rapid diagnostic test is for both present and past infection status, immunoglobulin M (IgM) indicates present history whereas immunoglobulin G (IgG) indicates past as shown above picture. Advantage Lepto IgG/IgM Combo Rapid Test is a visual, rapid, sensitive, …

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Voges Proskauer Test: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Positive and Negative Bacteria, and Limitations

Voges Proskauer Test Reagents, Negative and Positive Test Results of VP Tests

Voges-Proskauer (VP ) Test Voges Proskauer  test for identification to the species level of the following groups of organisms Principle of Voges Proskauer Test Organisms utilizing the butylene glycol pathway produce acetylmethylcarbinol (acetoin) and butanediol, neutral end products that raise the pH towards neutrality (pH …

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Methyl Red Test: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Positive and Negative Bacteria, and Limitations

Methyl Red Test-Positive and Negative Test Results

Introduction of Methyl Red Test The Methyl Red test is for enteric gram-negative rods, as part of identification to species level. Its short name is MR test. Principle of Methyl Red Test The methyl red (MR) test uses to determine if an organism is able …

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