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Introduction of Restriction fragment length polymorphisms (RFLPs) Restriction Fragment Length Polymorphisms (RFLPs) are a type of genetic variation found within the DNA of organisms, including humans. They were one of the earliest molecular techniques used for genetic analysis and have played a significant role in …
Introduction Pulsed-field gel electrophoresis (PFGE) is a powerful laboratory technique used in molecular biology and genetics to separate and analyze large DNA molecules, such as genomic DNA, by their size. PFGE is particularly valuable when studying organisms with complex genomes, such as bacteria, yeast, and …
Introduction Polymerase Chain Reaction, commonly known as PCR, is a powerful and widely used molecular biology technique that allows for the amplification of specific DNA sequences. Developed by Kary B. Mullis in 1983, PCR revolutionized the field of genetics and has become an essential tool …