Fungal Identification by NGS: Introduction, Principle, Test Methods, Test Procedure, Result-Interpretation, Clinical Significance, and Keynotes

PCR products of fungal DNA from pure culture after amplification with primers

Introduction Fungal identification by NGS (Next-Generation Sequencing) uses advanced DNA sequencing. Moreover, it rapidly detects diverse fungal species. It enhances diagnostic accuracy. Additionally, it supports personalized treatment decisions. Principle NGS sequences fungal DNA directly. Consequently, it generates millions of short DNA reads. Then, bioinformatics tools …

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Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

Introduction Next-generation sequencing (NGS) in Clinical Mycology is revolutionizing the diagnosis, typing, and antifungal resistance profiling of fungal pathogens. Application Here’s an overview of its applications: Candida spp.: Aspergillus spp.: CYP51A: Azole resistance due to mutations in ergosterol synthesis. Advantages of NGS in Clinical Mycology …

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Fungal Typing, Sequencing, and AFLP in Clinical Mycology Lab-Introduction, Purpose, Method, Application, and Conclusion

Various Candida species growth on Chromagar-Candida albicans, Candida krusie, Candida tropicalis, and Candida glabrata

Introduction to Advanced Molecular Techniques in Clinical Mycology Clinical mycology laboratories play a crucial role in diagnosing and managing fungal infections. Traditional methods like culture and microscopy are often complemented by advanced molecular techniques to improve the accuracy and speed of fungal identification. Among these …

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Next-Generation Sequencing (NGS) Platforms-Introduction, Principle, Types, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction of Next-Generation Sequencing (NGS) Platforms Next-Generation Sequencing (NGS) has transformed the field of genomics and our understanding of the intricacies of the genome. Here’s a brief introduction to the topic: What is Next-Generation Sequencing (NGS)? Next-Generation Sequencing (NGS), also known as high-throughput sequencing, is …

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Archaea-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Archaea, pronounced “ahr-kee-uh,” are a fascinating and diverse group of microorganisms that belong to the domain of life known as Archaea. They represent one of the three major domains of life, with the other two being Bacteria and Eukarya. They are distinct from both …

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