High-throughput sequencing Archives

Fungal Identification by NGS: Introduction, Principle, Test Methods, Test Procedure, Result-Interpretation, Clinical Significance, and Keynotes

February 22, 2025 by Medical Lab Notes

PCR products of fungal DNA from pure culture after amplification with primers

Introduction Fungal identification by NGS (Next-Generation Sequencing) uses advanced DNA sequencing. Moreover, it rapidly detects diverse fungal species. It enhances diagnostic accuracy. Additionally, it supports personalized treatment decisions. Principle NGS sequences fungal DNA directly. Consequently, it generates millions of short DNA reads. Then, bioinformatics tools …

Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

November 22, 2024 by Medical Lab Notes

Introduction Next-generation sequencing (NGS) in Clinical Mycology is revolutionizing the diagnosis, typing, and antifungal resistance profiling of fungal pathogens. Application Here’s an overview of its applications: Candida spp.: Aspergillus spp.: CYP51A: Azole resistance due to mutations in ergosterol synthesis. Advantages of NGS in Clinical Mycology …

The cost of whole-genome sequencing (WGS) for a fungus-Introduction, Keynotes, and Further Readings

June 1, 2024November 4, 2023 by Medical Lab Notes

Introduction of the cost of whole-genome sequencing (WGS) for a fungus Whole-genome sequencing (WGS) is a powerful technology that has revolutionized the fields of genetics, biology, and medicine. It involves determining the complete DNA sequence of an organism’s genome at a single time. This comprehensive …

Next-Generation Sequencing (NGS) Platforms-Introduction, Principle, Types, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

October 9, 2023 by Medical Lab Notes

Introduction of Next-Generation Sequencing (NGS) Platforms Next-Generation Sequencing (NGS) has transformed the field of genomics and our understanding of the intricacies of the genome. Here’s a brief introduction to the topic: What is Next-Generation Sequencing (NGS)? Next-Generation Sequencing (NGS), also known as high-throughput sequencing, is …