DNA sequencing

Fungal Identification by NGS: Introduction, Principle, Test Methods, Test Procedure, Result-Interpretation, Clinical Significance, and Keynotes

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PCR products of fungal DNA from pure culture after amplification with primers

Introduction Fungal identification by NGS (Next-Generation Sequencing) uses advanced DNA sequencing. Moreover, it rapidly detects diverse fungal species. It enhances diagnostic accuracy. Additionally, it supports personalized treatment decisions. Principle NGS sequences fungal DNA directly. Consequently, it generates millions of short DNA reads. Then, bioinformatics tools …

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Fungal Identification-Introduction, Conventional Method and MALDI TOF Method, Application, and Keynotes

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Conidia, phialides(sterigmata), metullae, and conidiophore of Penicillum in LPCB preparation of culture microscopic examination

Introduction Fungal identification plays a vital role in diagnosing infections. Mycologists use various methods to identify fungal species. Firstly, conventional techniques rely on morphology and culture characteristics. Additionally, molecular assays enhance detection accuracy. Researchers now employ advanced tools to expedite fungal identification. Moreover, timely identification …

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Rifampicin Resistant Indeterminate- Introduction, Result, Cause, Implication, and Summary

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Rifampicin Resistant Indeterminate result of Mycobacterium tuberculosis

Introduction “Rifampicin resistant indeterminate” refers to an unclear or inconclusive result in testing for resistance to the antibiotic rifampicin, often used in the treatment of tuberculosis (TB). Here’s a breakdown of the term: Rifampicin Resistant Result and Cause Indeterminate Result: This means the test did …

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Restriction fragment length polymorphisms (RFLPs)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

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Introduction of Restriction fragment length polymorphisms (RFLPs) Restriction Fragment Length Polymorphisms (RFLPs) are a type of genetic variation found within the DNA of organisms, including humans. They were one of the earliest molecular techniques used for genetic analysis and have played a significant role in …

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Multilocus sequence typing (MLST)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

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Introduction Multilocus Sequence Typing (MLST) is a molecular typing technique used in microbiology and genetics to classify and differentiate bacterial strains based on their genetic diversity. MLST is particularly valuable for studying the epidemiology and evolution of bacterial pathogens. Here is an introduction to the …

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Cladosporium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Introduction Cladosporium is a genus of fungi that belongs to the phylum Ascomycota. It is a diverse and widespread group of molds that can be found in various environments, including soil, plants, decaying organic matter, and indoor spaces. Cladosporium species (they) are known for their …

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Curvularia: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Curvularia Colony characteristics on SDA-Reverse side view

Introduction Curvularia is a genus of filamentous fungi belonging to the family Pleosporaceae, commonly found in soil, decaying plant material, and various environmental niches. These fungi are known for their distinctive curved or sigmoid-shaped conidia (asexual spores), which give the genus its name. Characteristics: Pathogenicity: …

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Candida parapsilosis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Candida parapsilosis growth on Candida CHROMagar

Introduction Candida parapsilosis is a species of yeast that belongs to the Candida genus, which includes various fungi commonly found in the environment and on the human body. Candida species are part of the normal human microbiota, residing on the skin, mucous membranes, and gastrointestinal …

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