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Introduction Sanger sequencing, developed by Frederick Sanger in 1977, is a chain-termination DNA sequencing method that remains the gold standard for molecular-level fungal identification.It is widely used to determine the exact nucleotide sequence of specific fungal genes (e.g., ITS, D1/D2, LSU, SSU, β-tubulin, calmodulin) to accurately identify fungal …
Introduction Accurate identification of fungi is critical for clinical, environmental, and epidemiological purposes. Traditional culture and morphology-based methods are time-consuming and may misidentify cryptic or closely related species. PCR amplification followed by Sanger sequencing of conserved genetic loci (e.g., ITS, 18S rRNA, 28S rRNA, β-tubulin, …
Introduction Fungal identification by NGS (Next-Generation Sequencing) uses advanced DNA sequencing. Moreover, it rapidly detects diverse fungal species. It enhances diagnostic accuracy. Additionally, it supports personalized treatment decisions. Principle NGS sequences fungal DNA directly. Consequently, it generates millions of short DNA reads. Then, bioinformatics tools …