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Introduction Accurate identification of fungi is critical for clinical, environmental, and epidemiological purposes. Traditional culture and morphology-based methods are time-consuming and may misidentify cryptic or closely related species. PCR amplification followed by Sanger sequencing of conserved genetic loci (e.g., ITS, 18S rRNA, 28S rRNA, β-tubulin, …
Introduction Accurate and timely identification of molds is essential for clinical microbiology, especially in cases of invasive fungal infections caused by Aspergillus, Fusarium, Scedosporium, Mucorales, and other filamentous fungi. Traditional methods based on morphology and phenotypic characteristics are slow, require expertise, and may misidentify cryptic …
Introduction Fungal infections represent a growing concern in both immunocompromised and immunocompetent individuals. Conventional identification methods, including culture, microscopy, and biochemical testing, often require several days and may lack sensitivity or specificity for certain opportunistic fungi. Molecular techniques such as PCR and sequencing have improved …