Fungal Identification

Fungal Identification by NGS: Introduction, Principle, Test Methods, Test Procedure, Result-Interpretation, Clinical Significance, and Keynotes

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PCR products of fungal DNA from pure culture after amplification with primers

Introduction Fungal identification by NGS (Next-Generation Sequencing) uses advanced DNA sequencing. Moreover, it rapidly detects diverse fungal species. It enhances diagnostic accuracy. Additionally, it supports personalized treatment decisions. Principle NGS sequences fungal DNA directly. Consequently, it generates millions of short DNA reads. Then, bioinformatics tools …

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Fungal Identification-Introduction, Conventional Method and MALDI TOF Method, Application, and Keynotes

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Conidia, phialides(sterigmata), metullae, and conidiophore of Penicillum in LPCB preparation of culture microscopic examination

Introduction Fungal identification plays a vital role in diagnosing infections. Mycologists use various methods to identify fungal species. Firstly, conventional techniques rely on morphology and culture characteristics. Additionally, molecular assays enhance detection accuracy. Researchers now employ advanced tools to expedite fungal identification. Moreover, timely identification …

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Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

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Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

Introduction Next-generation sequencing (NGS) in Clinical Mycology is revolutionizing the diagnosis, typing, and antifungal resistance profiling of fungal pathogens. Application Here’s an overview of its applications: Candida spp.: Aspergillus spp.: CYP51A: Azole resistance due to mutations in ergosterol synthesis. Advantages of NGS in Clinical Mycology …

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Rose Bengal Agar (RBA)- Introduction, Principle, Composition, Test Procedure, Colony Characteristics, Uses, and Keynotes

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Fungal growth on Rose Bengal Agar (RBA) at centre

Introduction Rose Bengal Agar (RBA) is a selective medium for isolating fungi. It contains rose bengal dye, which inhibits bacterial growth. This medium supports the growth of various fungi. Chloramphenicol in RBA further suppresses bacterial contamination. Scientists and clinicians use it for environmental and clinical …

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Slide Culture Technique for Fungi- Introduction, Requirement, Procedure, Uses, Disadvantage, and Keynotes

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Slide Culture Technique for Fungi- Introduction, Requirement, Procedure, Uses, Disadvantage, and Keynotes

Introduction The Slide Culture Technique for fungi allows detailed observation of fungal structures. It provides a clear view of spores and hyphae. This method uses a small block of agar on a slide. The technique is especially useful for identifying molds. You place a coverslip …

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Comparative Analysis of Capsule Expression in Cryptococcus neoformans: India Ink Visualization from Clinical CSF Samples versus SDA Agar Cultures

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Cryptococcus neoformans capsules in India Ink Preparation of Culture of organisms

Introduction of India Ink preparation Cryptococcus neoformans is an encapsulated yeast that causes cryptococcal meningitis, particularly in immunocompromised individuals. The capsule is a critical virulence factor, and its visualization is essential for diagnosis. This preparation is a common method used to visualize the capsule. Here, …

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Fonsecaea: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Fonsecaea: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Fonsecaea is a genus of fungi commonly associated with human and animal infections. It belongs to the class Eurotiomycetes and the order Chaetothyriales. The most clinically relevant species within this genus is Fonsecaea pedrosoi, which is known to cause chromoblastomycosis, a chronic and localized …

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Medical/Clinical Mycological Informations links: Introduction and List of Links

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Medical or Clinical Mycological Informations links- Introduction and List of Links

Introduction of Medical/Clinical Mycological Information links Nearly 1.5 million environmental fungal species out of which 8000 plant pathogens and 300 human pathogens respectively but such scenarios have changed rapidly in recent years. Prolonged steroid use, recent neutropenia, diabetes mellitus, cancer (AML), and rheumatologic conditions are the …

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