Molecular Typing of Fungi: Introduction, Common Molecular Typing Method, and Application

Various Candida species growth on HiCrome Candida Differential Agar

Introduction Molecular typing of fungi uses DNA-based methods to identify species and differentiate strains for clinical, epidemiological, and environmental studies. Key techniques include Internal Transcribed Spacer (ITS) sequencing (the standard for identification), Multilocus Sequence Typing (MLST), restriction fragment length polymorphism (RFLP), and Whole Genome Sequencing (WGS). These methods are crucial for tracking …

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Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

Introduction Next-generation sequencing (NGS) in Clinical Mycology is revolutionizing the diagnosis, typing, and antifungal resistance profiling of fungal pathogens. Application Here’s an overview of its applications: Candida spp.: Aspergillus spp.: CYP51A: Azole resistance due to mutations in ergosterol synthesis. Advantages of NGS in Clinical Mycology …

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