Nested PCR

Xpert MTB-XDR: Introduction, Principle, Procedure, Result-Interpretation, Uses, and Keynotes

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The image shows a GeneXpert software interface displaying an Xpert MTB/XDR test result with M. tuberculosis (MTB) detected and a specific drug resistance profile.Key Findings:MTB DETECTED: Positive for Mycobacterium tuberculosis complex.Low FLQ Resistance DETECTED: Indicates low-level resistance to fluoroquinolones (e.g., levofloxacin or moxifloxacin).Resistance NOT DETECTED: No mutations were found for isoniazid (INH), amikacin (AMK), kanamycin (KAN), capreomycin (CAP), or ethionamide (ETH).This result is consistent with a pre-XDR-TB diagnosis, as there is resistance to fluoroquinolones but susceptibility to second-line injectables.

Introduction The Xpert MTB/XDR assay is an advanced molecular diagnostic tool designed by Cepheid to rapidly detect Mycobacterium tuberculosis complex (MTBC) and its resistance to multiple first- and second-line drugs. Launched in 2021, it is categorized as a “low complexity” automated nucleic acid amplification test …

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Polymerase Chain Reaction (PCR): Introduction, Principle, Types, Application, and Keynotes

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Amplicons or DNA or RNA fragments of amplification or replication events

Introduction Polymerase Chain Reaction, commonly known as PCR, is a powerful and widely used molecular biology technique that allows for the amplification of specific DNA sequences. Developed by Kary B. Mullis in 1983, PCR revolutionized the field of genetics and has become an essential tool …

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