Detecting Low-Yield Target Fungal DNA from Bronchoalveolar Lavage Fluid: Sensitivity Limits of Conventional vs. Quantitative PCR
Introduction 0.5 ng/µL of DNA is detectable and can yield a positive result by PCR. In modern molecular diagnostics, PCR is incredibly sensitive. While 0.5 ng/µL might seem like a low concentration on a spectrophotometer (like a NanoDrop), it is actually a substantial amount of …
