Detecting Low-Yield Target Fungal DNA from Bronchoalveolar Lavage Fluid: Sensitivity Limits of Conventional vs. Quantitative PCR

Detecting Low-Yield Target Fungal DNA from Bronchoalveolar Lavage Fluid: Sensitivity Limits of Conventional vs. Quantitative PCR

Introduction 0.5 ng/µL of DNA is detectable and can yield a positive result by PCR. In modern molecular diagnostics, PCR is incredibly sensitive. While 0.5 ng/µL might seem like a low concentration on a spectrophotometer (like a NanoDrop), it is actually a substantial amount of …

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Acinetobacter: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Acinetobacter: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Acinetobacter is a genus of Gram-negative bacteria that are commonly found in the environment, including soil, water, and healthcare settings. While some species of Acinetobacter are harmless and part of the normal flora on human skin, others can cause opportunistic infections, particularly in healthcare-associated …

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Serratia fonticola: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment,Prevention, and Keynotes

Gram negative bacilli or rods of Serratia fonticola in Bronchoalveolar lavage (BAL) sample Gram staining

Introduction Serratia fonticola is a Gram-negative bacterium that belongs to the genus Serratia. It was first isolated and described in 1998 from water samples in a freshwater spring in California, USA. Since its discovery, S. fonticola has been identified in various environmental sources, including soil, …

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