Limitations Archives - Medical Notes

Scrub Typhus Antibody Test-Introduction, Principle, Test Requirements, Procedure, Result Interpretation, Test Result, Test Method, Limitations, and Keynotes

February 27, 2023February 24, 2023 by Medical Lab Notes

Scrub Typhus Antibody Test-Negative and Positive Results

Introduction of Scrub Typhus Scrub typhus and typhoid are quite different but common thing is that both cover fever. Scrub typhus also known as bush typhus, is a disease caused by the intracellular parasite, Orientia ( formerly Rickettsia) tsutsugamushi and typhoid by Salmonella enterica serotype Typhi. Both causative agents are bacteria. Scrub …

Auramine-Phenol Fluorochrome Staining: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation, Limitations, and Keynotes

March 4, 2023February 20, 2023 by Medical Lab Notes

Acid fast bacilli (AFB) of Mycobacterium tuberculosis in Auramine-phenol fluorochrome Staining

Introduction of Auramine-Phenol stain Auramine-Phenol is a fluorochrome stain used to visualize acid-fast structures of various microorganisms, especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores. Ziehl-Neelsen (hot), and Kinyoun (cold) are still widely used methods to detect acid-fast structures …

Haemophilus Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Limitations, and Keynotes

March 4, 2023February 19, 2023 by Medical Lab Notes

Use of bacitracin 10 U on chocolate agar for screening Haemophilus of sputum culture

Introduction of Haemophilus Serotyping Haemophilus Serotyping helps to differentiate serotypes of Haemophilus influenzae. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, encapsulated strains of Haemophilus influenzae may …

Decarboxylase Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

February 17, 2023February 2, 2023 by Medical Lab Notes

Decarboxylase Test-Positive and Negative Results Demonstration

Principle of Decarboxylase-Dihydrolase Test Lysine, ornithine, and arginine are the most common amino acids which use to detect an organism’s ability to decarboxylate or hydrolyze an amino acid in a decarboxylase medium, forming an amine that produces an alkaline pH. The basal medium for the …

Methyl-α-D-glucopyranoside (MGP) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

February 17, 2023February 2, 2023 by Medical Lab Notes

Methyl-α-D-glucopyranoside (MGP) Test- Positive and Negative Results Demonstration

Introduction of Methyl-α-D-glucopyranoside (MGP)Test Methyl-α-D-glucopyranoside (MGP) test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliﬂavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from Enterococcus gallinarum and Enterococcus casseliﬂavus, while not important for purposes of control of …

Indoxyl Acetate Disk Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

February 17, 2023February 2, 2023 by Medical Lab Notes

Indoxyl Acetate Disk Test-Positive and Negative Results

Introduction of Indoxyl Acetate Disk Test Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …

VDRL Test: Introduction, Principle, Procedure, Result-Interpretation,Limitations, and Keynotes

November 29, 2022July 31, 2022 by Medical Lab Notes

Introduction of VDRL Test VDRL stands for Veneral disease research laboratory. The laboratory was renamed the Treponemal Pathogenesis and Immunology Branch of the United States Public Health Service. The Venereal Disease Research Laboratory (VDRL) test is one of two variations of micro flocculation procedures used for serological testing …