Stain

Mycobacteria Growth Indicator Tube (MGIT) Culture Outcomes: Introduction, Identification, Causes of Contamination, and Keynotes

by
Mycobacteria Growth Indicator Tube (MGIT)-Positive

Introduction The Mycobacteria Growth Indicator Tube (MGIT) system is a high-throughput, automated method optimized for the rapid isolation and detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria (NTM).  [Initial Stage]     Oxygen is high —–> Quenches sensor —–> NO FLUORESCENCE [Bacterial Growth]  Oxygen consumed —-> Frees …

Read more

Auramine-Phenol Fluorochrome Staining: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation, Limitations, and Keynotes

by
Acid fast bacilli (AFB) of Mycobacterium tuberculosis in Auramine-phenol fluorochrome Staining

Introduction of Auramine-Phenol stain Auramine-Phenol is a fluorochrome stain used to visualize acid-fast structures of various microorganisms, especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores. Ziehl-Neelsen (hot), and Kinyoun (cold) are still widely used methods to detect acid-fast structures …

Read more