Multiplex Real-Time PCR in Infectious Disease Diagnostics: A New Era of Rapid and Comprehensive Detection

The increasing burden of infectious diseases demands diagnostic solutions that are not only rapid but also highly sensitive and comprehensive. Conventional diagnostic methods often fall short due to time delays and limited pathogen coverage. In this context, multiplex real-time PCR technologies are revolutionizing infectious disease …

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TrueNat for Tuberculosis: Introduction, Principle, Procedure, Result-Interpretation, application, and Keynotes

Introduction TrueNat is a rapid, chip-based, and portable molecular diagnostic test. It is developed by Molbio Diagnostics for the detection of tuberculosis (TB) and rifampicin (RIF) resistance. It is the first molecular point-of-care test for TB recommended by the World Health Organization (WHO).  Principle The TrueNat system …

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COVID-19 Antigen Test- Introduction, Principle, Test Requirements, Procedure, Result Interpretation, Clinical Significance and Disadvantage

COVID-19 Antigen Test-Positive

Introduction of COVID-19 Antigen Test SARS-CoV-2 antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is used as a screening test on asymptomatic persons to detect or exclude Coronavirus Disease 2019 (COVID-19) or to determine whether a person who previously was diagnosed with COVID-19 …

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Candida-Antibody Testing-Introduction, Principle, Purpose, Types of antibody tests, Limitation, and More

Candida albicans growth on Biphasic medium (BHI agar and broth)-right (turbid)- No growth on left (clear)

Introduction Candida antibody testing measures immunoglobulins (IgG, IgA, and IgM) antibodies in the blood to help detect current or past Candida infections. These antibodies are produced in response to the presence of Candida in the body, assisting the immune system in fighting off the fungus. Principle Purpose …

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Direct Microscopy Examination of Clinical Samples- Introduction, Purpose and Benefits, Methods, Applications, and Limitation

Direct Microscopy Examination of Clinical Samples- Introduction, Purpose and Benefits, Methods, Applications, and Limitation

Introduction of Direct Microscopy Examination of Clinical Samples Direct microscopy examination of clinical samples, or wet mount examination, involves examining clinical specimens under a microscope without prior staining or culturing. This technique provides a rapid assessment of the presence and morphology of microbes, facilitating preliminary diagnosis and …

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HIV, HBsAg and HCV ELISA: Introduction, Test Results, Test methods, Clinical Significance, and Keynotes

HIV, HBsAg and HCV ELISA: Introduction, Test Results, Test methods, Clinical Significance, and Keynotes

Introduction HIV, HBsAg, and HCV ELISA are three distinct diagnostic tests used in the field of medicine and clinical diagnostics to detect specific viral infections. Here’s an introduction to each of these tests: These ELISA tests are essential tools in the diagnosis and monitoring of …

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Malarial Antigen Test-Introduction, Test Result, Test Method, Clinical Significance, and Keynotes

Malarial Antigen Test-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Introduction The Malarial Antigen Test, often referred to as the Malaria Rapid Diagnostic Test (RDT) or Malaria Antigen Rapid Test, is a diagnostic tool used to detect the presence of malaria parasites in a patient’s blood. Malaria is a serious and sometimes life-threatening infectious disease …

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Immunochromatography: Introduction, Principle, Procedure, Result-Interpretation, Uses and Keynotes

Rapid tests based on Immunochromatography- Hepatitis A virus, Hepatitis E virus, Syphilis rapid test, ToRCH Rapid test and more

Introduction Immunochromatography, also known as lateral flow immunoassay or rapid test, is a widely used diagnostic technique that allows for the rapid and qualitative detection of specific target substances, such as antigens or antibodies, in various biological samples. It is a simple, cost-effective, and user-friendly …

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