TrueNat for Tuberculosis: Introduction, Principle, Procedure, Result-Interpretation, application, and Keynotes

Introduction TrueNat is a rapid, chip-based, and portable molecular diagnostic test. It is developed by Molbio Diagnostics for the detection of tuberculosis (TB) and rifampicin (RIF) resistance. It is the first molecular point-of-care test for TB recommended by the World Health Organization (WHO).  Principle The TrueNat system …

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Immunofluorescent Detection of Mycobacteria Using Polyclonal Anti-BCG Antibody Staining: Introduction, Principle, Clinical Significance, Advantage, Disadvantage, and Keynotes

Introduction Immunofluorescent staining with polyclonal anti-BCG antibodies is a rapid and specific technique used to detect Mycobacterium species, particularly in clinical and research settings. These antibodies recognize antigenic components of Mycobacterium bovis BCG strain and cross-react with members of the M. tuberculosis complex. The method …

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