Yeast Identification by MALDI-TOF (VITEK): Introduction, Principle, Clinical Significance, and Keynotes

Yeast Identification by MALDI-TOF (VITEK): Introduction, Principle, Clinical Significance, and Keynotes

Introduction Yeasts, especially Candida species, are among the most important causes of opportunistic fungal infections in humans. Accurate and rapid identification is crucial for guiding antifungal therapy and improving patient outcomes. Traditional phenotypic methods are time-consuming and often misidentify cryptic species. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight …

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Candida auris PCR-Directly on Clinical Samples: Introduction, Principle, Clinical Significance, and Keynotes

Candida auris PCR-Directly on Clinical Samples: Introduction, Principle, Clinical Significance, and Keynotes

Introduction Candida auris is an emerging multidrug-resistant yeast responsible for outbreaks of healthcare-associated infections worldwide. It is difficult to identify by conventional culture and biochemical methods, often misidentified as other Candida species (C. haemulonii, C. famata). Rapid and accurate detection is essential to initiate infection …

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Multiplex PCR assay-Directly on Respiratory samples:Introduction, Principle, Clinical Significance, and Keynotes

Introduction Respiratory tract infections are caused by a wide range of pathogens, including viruses, bacteria, and fungi. Conventional diagnostic methods such as culture, microscopy, and antigen detection are time-consuming and often lack sensitivity. Multiplex polymerase chain reaction (PCR) assays, applied directly to respiratory samples (sputum, …

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