ONPG Test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

ONPG Test Negative (left) and Positive Bacteria (right)

Introduction of ONPG Test ONPG test is useful in differentiating members of the family  Enterobacteriaceae and genera Neisseria based on b-D-galactosidase activity. ONPG stands for o-Nitrophenyl-b-D-galactopyranoside. Here we discuss on ONPG Disk Test which is simple and easy to perform. Principle The ability of an organism to ferment lactose …

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