Multiplex PCR assay-Directly on CSF: Introduction, Principle, Clinical Significance, and Keynotes

Introduction Multiplex Polymerase Chain Reaction (PCR) directly on Cerebrospinal Fluid (CSF) is an advanced molecular diagnostic technique designed to detect multiple pathogens simultaneously from a single CSF sample. It offers rapid, sensitive, and specific identification of bacterial, viral, and fungal agents responsible for central nervous …

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Multiplex PCR Assay-Directly on Blood for Fungi: Introduction, Principle, Clinical Significance, and Keynotes

Introduction Fungal bloodstream infections (fungemia) are life-threatening, especially in immunocompromised and oncology patients. Conventional methods like blood culture are slow, often requiring several days for growth and identification. Multiplex PCR directly on blood offers rapid, sensitive, and simultaneous detection of multiple fungal pathogens without the …

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