Multiplex PCR assay-Directly on CSF: Introduction, Principle, Clinical Significance, and Keynotes

Introduction Multiplex Polymerase Chain Reaction (PCR) directly on Cerebrospinal Fluid (CSF) is an advanced molecular diagnostic technique designed to detect multiple pathogens simultaneously from a single CSF sample. It offers rapid, sensitive, and specific identification of bacterial, viral, and fungal agents responsible for central nervous …

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Comparison: Multiplex PCR vs Blood Culture vs Biomarker Tests (β-D-Glucan / CrAg)B

Multiplex PCR Setup

Introduction of Comparison: Multiplex PCR vs Blood Culture vs Biomarker Tests The diagnosis of invasive fungal infections remains a major clinical challenge, especially in high-risk patients such as those with cancer, transplants, or critical illness. Conventional blood culture is still the gold standard but suffers …

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