Blood Iron Test: Introduction, Principle,Test Procedure,Test Result, Unit, Reference Range, Testing Methods,and Keynotes


The Blood Iron Test, also known as the Serum Iron Test, is a medical examination that measures the level of iron in your blood. Iron is an essential mineral that plays a crucial role in carrying oxygen throughout the body and is a key component of hemoglobin, the protein in red blood cells responsible for transporting oxygen from the lungs to tissues and organs.

The Blood Iron Test is often ordered as part of a series of blood tests to assess a person’s iron status and help diagnose various conditions related to iron levels, such as:

  1. Iron deficiency anemia: A condition in which the body lacks sufficient iron to produce an adequate amount of hemoglobin, leading to a decrease in red blood cell production and subsequent anemia.
  2. Hemochromatosis: A hereditary disorder where the body absorbs and stores too much iron, which can lead to iron overload and damage to organs like the liver, heart, and pancreas.
  3. Iron poisoning: A condition that can occur from accidental or intentional ingestion of excessive iron supplements, which can be toxic.
  4. Thalassemia: A group of inherited blood disorders that affect the production of hemoglobin, leading to anemia.


The principle of the Blood Iron Test using CLIA (Chemiluminescence Immunoassay) is based on the specific interaction between iron-binding proteins and antibodies labeled with chemiluminescent substances. This test is used to determine the concentration of iron in the blood serum.

Tset Procedure

During the test, a sample of blood is taken from a vein, typically from the arm. The serum iron level is then measured in micrograms per deciliter (mcg/dL) of blood. The test may be combined with other measures, such as total iron-binding capacity (TIBC) and ferritin levels, to provide a more comprehensive assessment of iron status.

Interpretation of the results will depend on the individual’s age, sex, and other factors, and it should always be done by a qualified healthcare professional. Abnormal iron levels can indicate various conditions, so further investigations may be required for proper diagnosis and treatment.

The procedure for a blood iron test involves the collection of a blood sample and subsequent analysis in a laboratory. Here’s a step-by-step outline of the typical procedure:

  1. Preparation:
    • No specific preparation is usually required for a blood iron test. However, it’s always a good idea to follow any instructions given by your healthcare provider, such as fasting requirements or medication restrictions, if applicable.
  2. Blood Sample Collection:
    • A healthcare professional, usually a phlebotomist or a nurse, will perform the blood draw. They will use a sterile needle to draw blood from a vein, typically from the inside of your elbow or the back of your hand. Before the procedure, they may clean the area with an antiseptic and may apply a tourniquet to help locate the vein.
  3. Filling the Blood Collection Tube:
    • The blood is drawn into a specialized blood collection tube. For a blood iron test, a tube without any additives (a plain red-top tube) is commonly used to obtain a serum sample. The tourniquet is released once the blood is flowing into the tube.
  4. Bandaging and Labeling:
    • After the blood has been collected, the needle is removed from your arm, and a small bandage or cotton ball with tape is placed over the puncture site to stop bleeding. The tube is labeled with your identification details for proper tracking in the laboratory.
  5. Transport to the Laboratory:
    • The blood sample is then sent to the laboratory for analysis. It is crucial to ensure the proper handling and transportation of the sample to maintain its integrity for accurate testing.
  6. Analysis in the Laboratory:
    • In the laboratory, the blood sample is processed to separate the serum (the liquid part of the blood) from the cellular components. The serum is used for measuring iron levels.
  7. Blood Iron Test Using CLIA (Chemiluminescence Immunoassay) or Other Methods:
    • The laboratory technician will use the collected serum and perform the blood iron test. As mentioned earlier, this may involve the use of a chemiluminescence immunoassay (CLIA) or other methods to measure the iron concentration in the serum.
  8. Reporting Results:
    • Once the analysis is complete, the results are generated, indicating the iron concentration in the blood serum. These results are typically reported in units of micrograms per deciliter (mcg/dL) or other appropriate units.
  9. Interpretation and Follow-up:
    • The test results will be interpreted by a healthcare professional, who will consider your medical history, symptoms, and other relevant factors. Abnormal iron levels may require further evaluation and follow-up to determine the underlying cause and plan appropriate treatment if necessary.

Test Result, Unit, Reference Range,and Test Methods

Blood Iron Test -Introduction, Principle,Test Procedure,Test Result, Unit, Reference Range, Testing Methods,and Keynotes
Fig. Blood Iron Test -Test Result, Unit, Reference Range, and Test Methods


Keynotes on the Blood Iron Test include:

  1. Purpose: The Blood Iron Test, also known as the Serum Iron Test, is conducted to measure the concentration of iron in the blood serum. It helps assess the body’s iron status and diagnose iron-related disorders.
  2. Iron’s Role: Iron is an essential mineral needed for the production of hemoglobin, which carries oxygen in red blood cells. Proper iron levels are crucial for overall health and energy production.
  3. Testing Method: The test is performed in a clinical laboratory using a blood sample drawn from a vein, typically in the arm. The serum is separated and analyzed for iron concentration.
  4. Specimen Collection: Fasting is not typically required for the Blood Iron Test. The blood sample is collected in a plain red-top tube without any additives.
  5. Reference Ranges: The normal range for serum iron levels can vary depending on age, sex, and other factors. The reference range is usually provided on the lab report for easy interpretation.
  6. Interpretation: Low serum iron levels may indicate iron deficiency anemia or chronic diseases, while high levels may suggest conditions like hemochromatosis or excessive iron intake.
  7. Additional Tests: The Blood Iron Test may be combined with other tests, such as total iron-binding capacity (TIBC) and ferritin levels, for a comprehensive assessment of iron status.
  8. Iron Supplementation: If iron deficiency is diagnosed, healthcare providers may recommend iron supplements or dietary changes to correct the deficiency.
  9. Precautions: Iron supplements should not be taken without proper medical supervision, as excessive iron can be harmful. Self-diagnosis or self-treatment based on test results is not advisable.
  10. Interpretation by Professionals: The Blood Iron Test results should always be interpreted by a qualified healthcare professional, who considers the patient’s medical history, symptoms, and other laboratory findings for accurate diagnosis and treatment.
  11. Monitoring and Follow-up: Patients with abnormal iron levels may require regular monitoring and follow-up to ensure appropriate management and improvement of their iron status.

Further Readings

  1. Lab Tests Online: This is a reliable resource provided by the American Association for Clinical Chemistry (AACC) that offers detailed information about various laboratory tests, including the Blood Iron Test. Visit their website at and search for “serum iron” or “blood iron test” to access relevant articles.
  2. National Institutes of Health (NIH): The NIH provides a wealth of information on medical topics, including iron metabolism and iron-related disorders. You can find relevant articles and research papers on the NIH website ( by searching for terms like “iron metabolism,” “iron deficiency anemia,” or “hemochromatosis.”
  3. PubMed: PubMed is a free database of biomedical literature, maintained by the National Library of Medicine (NLM). You can search for scientific articles related to the Blood Iron Test or iron-related conditions by visiting
  4. Medical Journals: Various medical journals publish research articles and reviews related to iron metabolism, iron deficiency anemia, hemochromatosis, and other iron-related disorders. Some reputable journals in this field include the Journal of Clinical Investigation, Blood, and the American Journal of Hematology, among others.
  5. Books and Textbooks: There are numerous textbooks and medical books dedicated to hematology and laboratory medicine that cover the Blood Iron Test and related topics. Check your local library or online bookstores for titles focusing on hematology and laboratory diagnostics.
  6. Professional Organizations: Organizations like the American Society of Hematology (ASH) and the American Association for Clinical Chemistry (AACC) often publish guidelines and position statements related to iron testing and management.

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