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Introduction of Vitamin-B12 Vitamin B12 is naturally present in animal foods like fish, meat, poultry, egg, milk, and other dairy product. It is also available in foods or supplements. It requires the formation of red blood cells (RBCs) and DNA which play a key role …
Introduction of Thyroid Function Test (TFT) If you are suffering from any of the symptoms stated in the table (Differences between Hypothyroidism and Hyperthyroidism), ask for a thyroid function test (TFT). Hypothyroidism is a condition in which the thyroid-stimulating hormone (TSH) level in your body …
Introduction of Occult Blood and Helicobacter pylori Antigen Stool Test Occult Blood and Helicobacter pylori Antigen Stool Tests are clinically important for the diagnosis of diseases. Fecal occult blood test positive is common in hookworm infestation. H. pylori antigen stool test is clear from the …
Principle of Decarboxylase-Dihydrolase Test Lysine, ornithine, and arginine are the most common amino acids which use to detect an organism’s ability to decarboxylate or hydrolyze an amino acid in a decarboxylase medium, forming an amine that produces an alkaline pH. The basal medium for the …
Introduction of Methyl-α-D-glucopyranoside (MGP)Test Methyl-α-D-glucopyranoside (MGP) test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliflavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from Enterococcus gallinarum and Enterococcus casseliflavus, while not important for purposes of control of …
Introduction of Indoxyl Acetate Disk Test Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …
Introduction of Hippurate Hydrolysis Test The Hippurate Hydrolysis Test is applied to determine the ability of the organism to produce hippuricase which hydrolyzes the substrate hippurate. It is useful in the identification of Streptococcus agalactiae, Campylobacter jejuni, Listeria monocytogenes, and Gardnerella vaginalis. Principle of the Test The end products …
Introduction of LAP (Leucine Aminopeptidase) Test LAP stands for leucine aminopeptidase which is an enzyme useful for definitive identification of catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group like Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive). Principle of LAP test Hydrolysis of the leucine-pnaphthylamide substrate by …